scholarly journals Three-dimensional super-resolution imaging for fluorescence emission difference microscopy

AIP Advances ◽  
2015 ◽  
Vol 5 (8) ◽  
pp. 084901 ◽  
Author(s):  
Shangting You ◽  
Cuifang Kuang ◽  
Shuai Li ◽  
Xu Liu ◽  
Zhihua Ding
Keyword(s):  
Three Dimensional ◽  
Fluorescence Emission ◽  
Super Resolution ◽  
Resolution Imaging

Super-resolution imaging using a three-dimensional metamaterials nanolens

2010 ◽  
Vol 96 (2) ◽  
pp. 023114 ◽  
Author(s):  
B. D. F. Casse ◽  
W. T. Lu ◽  
Y. J. Huang ◽  
E. Gultepe ◽  
L. Menon ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

2021 ◽  
Author(s):  
Anna Loeschberger ◽  
Yauheni Novikau ◽  
Ralf Netz ◽  
Marie-Christin Spindler ◽  
Ricardo Benavente ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Brain Slices ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Living Cells ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Spatiotemporal Resolution ◽  
Coated Pits ◽  
Resolution Imaging

Three-dimensional (3D) multicolor super-resolution imaging in the 50-100 nm range in fixed and living cells remains challenging. We extend the resolution of structured illumination microscopy (SIM) by an improved nonlinear iterative reconstruction algorithm that enables 3D multicolor imaging with improved spatiotemporal resolution at low illumination intensities. We demonstrate the performance of dual iterative SIM (diSIM) imaging cellular structures in fixed cells including synaptonemal complexes, clathrin coated pits and the actin cytoskeleton with lateral resolutions of 60-100 nm with standard fluorophores. Furthermore, we visualize dendritic spines in 70 micrometer thick brain slices with an axial resolution < 200 nm. Finally, we image dynamics of the endoplasmatic reticulum and microtubules in living cells with up to 255 frames/s.

Super-resolution imaging reveals three-dimensional folding dynamics of the  -globin locus upon gene activation

2012 ◽  
Vol 125 (19) ◽  
pp. 4630-4639 ◽  
Author(s):  
M. P. C. van de Corput ◽  
E. de Boer ◽  
T. A. Knoch ◽  
W. A. van Cappellen ◽  
A. Quintanilla ◽  
...  
Keyword(s):  
Gene Activation ◽  
Three Dimensional ◽  
Super Resolution ◽  
Folding Dynamics ◽  
Resolution Imaging

scholarly journals PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

2012 ◽  
Vol 20 (5) ◽  
pp. 4957 ◽  
Author(s):  
Ignacio Izeddin ◽  
Mohamed El Beheiry ◽  
Jordi Andilla ◽  
Daniel Ciepielewski ◽  
Xavier Darzacq ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Single Molecule ◽  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

Three-Dimensional Super-resolution Imaging of Single Nanoparticles Delivered by Pipettes

ACS Nano ◽  
2017 ◽  
Vol 11 (10) ◽  
pp. 10529-10538 ◽  
Author(s):  
Yun Yu ◽  
Vignesh Sundaresan ◽  
Sabyasachi Bandyopadhyay ◽  
Yulun Zhang ◽  
Martin A. Edwards ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

Three-dimensional super-resolution imaging with a double-helix microscope

2009 ◽  
Author(s):  
Sri Rama Prasanna Pavani ◽  
Rafael Piestun ◽  
Michael A. Thompson ◽  
Julie S. Biteen ◽  
W. E. Moerner
Keyword(s):  
Double Helix ◽  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Optical Phenomena in Mesoscale Dielectric Particles

Photonics ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 591
Author(s):  
Oleg V. Minin ◽  
Igor V. Minin
Keyword(s):  
Three Dimensional ◽  
Field Enhancement ◽  
Super Resolution ◽  
Size Parameter ◽  
Dielectric Particles ◽  
Mesoscale Structures ◽  
Dimensional Shape ◽  
Resolution Imaging ◽  
Physical Phenomena ◽  
Three Dimensional Shape

During the last decade, new unusual physical phenomena have been discovered in studying the optics of dielectric mesoscale particles of an arbitrary three-dimensional shape with the Mie size parameter near 10 (q~10). The paper provides a brief overview of these phenomena from optics to terahertz, plasmonic and acoustic ranges. The different particle configurations (isolated, regular or Janus) are discussed, and the possible applications of such mesoscale structures are briefly reviewed herein in relation to the field enhancement, nanoparticle manipulation and super-resolution imaging. The number of interesting applications indicates the appearance of a new promising scientific direction in optics, terahertz and acoustic ranges, and plasmonics. This paper presents the authors’ approach to these problems.

scholarly journals Restoring single-molecule localizations with wavefront sensing adaptive optics for deep-tissue super-resolution imaging

2021 ◽  
Author(s):  
Sanghyeon Park ◽  
Yonghyeon Jo ◽  
Minsu Kang ◽  
Jin Hee Hong ◽  
Sangyoon Ko ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Single Molecule ◽  
Fluorescence Emission ◽  
Super Resolution ◽  
Light Modulator ◽  
Application Range ◽  
Wavefront Distortion ◽  
Resolution Imaging ◽  
Localization Precision ◽  
Major Factors

Specimen-induced aberration has been one of the major factors limiting the imaging depth in single-molecule localization microscopy (SMLM). In this study, we measured the wavefront of intrinsic reflectance signal at the fluorescence emission wavelength to construct a time-gated reflection matrix and find complex tissue aberration without resorting to fluorescence detection. Physically correcting the identified aberration via wavefront shaping with a liquid-crystal spatial light modulator (SLM) enables super-resolution imaging even when the aberration is too severe for initiating localization processes. We demonstrate the correction of complex tissue aberration, the root-mean-square (RMS) wavefront distortion of which is more than twice the 1 rad limit presented in previous studies; this leads to the recovery of single molecules by 77 times increased localization number. We visualised dendritic spines in mouse brain tissues and early myelination processes in a whole zebrafish at up to 102 μm depth with 28-39 nm localization precision. The proposed approach can expand the application range of SMLM to thick samples that cause the loss of localization points owing to severe aberration.

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