scholarly journals Long-term time-lapse microscopy of C. elegans post-embryonic development

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Nicola Gritti ◽  
Simone Kienle ◽  
Olga Filina ◽  
Jeroen Sebastiaan van Zon
Keyword(s):  
Embryonic Development ◽  
Time Lapse ◽  
C Elegans ◽  
Time Lapse Microscopy

A microfluidic system for long-term time-lapse microscopy studies of mycobacteria

Tuberculosis ◽  
2012 ◽  
Vol 92 (6) ◽  
pp. 489-496 ◽  
Author(s):  
Solmaz A. Golchin ◽  
James Stratford ◽  
Richard J. Curry ◽  
Johnjoe McFadden
Keyword(s):  
Time Lapse ◽  
Microfluidic System ◽  
Time Lapse Microscopy

scholarly journals Isolating live cells after high-throughput, long-term, time-lapse microscopy

2019 ◽  
Vol 17 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Scott Luro ◽  
Laurent Potvin-Trottier ◽  
Burak Okumus ◽  
Johan Paulsson
Keyword(s):  
High Throughput ◽  
Time Lapse ◽  
Live Cells ◽  
Time Lapse Microscopy

scholarly journals Multi-generational Analysis and Manipulation of Chromosomes in a Polyploid Cyanobacterium

2019 ◽  
Author(s):  
Kristin A. Moore ◽  
Jian Wei Tay ◽  
Jeffrey C. Cameron
Keyword(s):  
Dna Replication ◽  
Cell Growth ◽  
Photosynthetic Bacteria ◽  
Bacterial Species ◽  
Genetic Material ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Life On Earth ◽  
Microbial Dna

ABSTRACTFaithful inheritance of genetic material from one generation to the next is an essential process for all life on earth. Much of what is known about microbial DNA replication and inheritance has been learned from a small number of bacterial species that share many common traits. Whether these pathways are conserved across the great diversity of the microbiome remains unclear. To address this question, we studied chromosome dynamics in a polyploid photosynthetic bacteria using single cell, time-lapse microscopy over multi-generation lineages in conjunction with inducible CRISPR-interference and fluorescent chromosome labeling. With this method we demonstrated the long-term consequences of manipulating parameters such as cell growth, cell division, and DNA replication and segregation on chromosome regulation in a polyploid bacterial species. We find that these bacteria are surprisingly resilient to chromosome disruption resulting in continued cell growth when DNA replication is inhibited and even in the complete absence of chromosomes.

Encapsulated Petri dish system for single-cell drug delivery and long-term time lapse microscopy

2007 ◽  
Vol 371 (2) ◽  
pp. 208-214 ◽  
Author(s):  
Marcelo Salierno ◽  
Ricardo Cabrera ◽  
Oscar Filevich ◽  
Roberto Etchenique
Keyword(s):  
Drug Delivery ◽  
Single Cell ◽  
Petri Dish ◽  
Time Lapse ◽  
Time Lapse Microscopy

In-vivo third-harmonic generation microscopy at 1550nm three-dimensional long-term time-lapse studies in living C. elegans embryos

2011 ◽  
Author(s):  
Rodrigo Aviles-Espinosa ◽  
Susana I. C. O. Santos ◽  
Andreas Brodschelm ◽  
Wilhelm G. Kaenders ◽  
Cesar Alonso-Ortega ◽  
...  
Keyword(s):  
Harmonic Generation ◽  
Three Dimensional ◽  
Time Lapse ◽  
Third Harmonic Generation ◽  
Third Harmonic ◽  
C Elegans ◽  
Third Harmonic Generation Microscopy

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

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