Encapsulated Petri dish system for single-cell drug delivery and long-term time lapse microscopy

2007 ◽  
Vol 371 (2) ◽  
pp. 208-214 ◽  
Author(s):  
Marcelo Salierno ◽  
Ricardo Cabrera ◽  
Oscar Filevich ◽  
Roberto Etchenique
Keyword(s):  
Drug Delivery ◽  
Single Cell ◽  
Petri Dish ◽  
Time Lapse ◽  
Time Lapse Microscopy

scholarly journals Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Han Wang ◽  
Gloria M. Conover ◽  
Song-I Han ◽  
James C. Sacchettini ◽  
Arum Han
Keyword(s):  
Drug Treatment ◽  
Single Cell ◽  
Mycobacterium Smegmatis ◽  
Culture Media ◽  
Low Cost ◽  
Bacterial Pathogen ◽  
Growth Dynamics ◽  
Time Lapse ◽  
Cell Phenotype

AbstractAnalysis of growth and death kinetics at single-cell resolution is a key step in understanding the complexity of the nonreplicating growth phenotype of the bacterial pathogen Mycobacterium tuberculosis. Here, we developed a single-cell-resolution microfluidic mycobacterial culture device that allows time-lapse microscopy-based long-term phenotypic visualization of the live replication dynamics of mycobacteria. This technology was successfully applied to monitor the real-time growth dynamics of the fast-growing model strain Mycobacterium smegmatis (M. smegmatis) while subjected to drug treatment regimens during continuous culture for 48 h inside the microfluidic device. A clear morphological change leading to significant swelling at the poles of the bacterial membrane was observed during drug treatment. In addition, a small subpopulation of cells surviving treatment by frontline antibiotics was observed to recover and achieve robust replicative growth once regular culture media was provided, suggesting the possibility of identifying and isolating nonreplicative mycobacteria. This device is a simple, easy-to-use, and low-cost solution for studying the single-cell phenotype and growth dynamics of mycobacteria, especially during drug treatment.

A Petri-Dish with Micromolded Pattern as a Coordinate Indicator for Live-Cell Time Lapse Microscopy

2021 ◽  
Author(s):  
Kyungwon Yun ◽  
Dohyun Park ◽  
Myeongwoo Kang ◽  
Jiyoung Song ◽  
Yoojin Chung ◽  
...  
Keyword(s):  
Petri Dish ◽  
Time Lapse ◽  
Live Cell ◽  
Time Lapse Microscopy

A microfluidic system for long-term time-lapse microscopy studies of mycobacteria

Tuberculosis ◽  
2012 ◽  
Vol 92 (6) ◽  
pp. 489-496 ◽  
Author(s):  
Solmaz A. Golchin ◽  
James Stratford ◽  
Richard J. Curry ◽  
Johnjoe McFadden
Keyword(s):  
Time Lapse ◽  
Microfluidic System ◽  
Time Lapse Microscopy

scholarly journals Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy

2011 ◽  
Vol 7 (1) ◽  
pp. 80-88 ◽  
Author(s):  
Jonathan W Young ◽  
James C W Locke ◽  
Alphan Altinok ◽  
Nitzan Rosenfeld ◽  
Tigran Bacarian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Cell Gene Expression ◽  
Gene Expression Dynamics ◽  
Cell Gene

Time‐Lapse Microscopy Approaches to Track Cell Cycle and Lineage Progression at the Single‐Cell Level

2013 ◽  
Vol 64 (1) ◽  
Author(s):  
Rachel J. Errington ◽  
Sally C. Chappell ◽  
Imtiaz A. Khan ◽  
Nuria Marquez ◽  
Marie Wiltshire ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Time Lapse ◽  
Single Cell Level ◽  
Cell Level ◽  
Time Lapse Microscopy

scholarly journals Isolating live cells after high-throughput, long-term, time-lapse microscopy

2019 ◽  
Vol 17 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Scott Luro ◽  
Laurent Potvin-Trottier ◽  
Burak Okumus ◽  
Johan Paulsson
Keyword(s):  
High Throughput ◽  
Time Lapse ◽  
Live Cells ◽  
Time Lapse Microscopy

scholarly journals Multi-generational Analysis and Manipulation of Chromosomes in a Polyploid Cyanobacterium

2019 ◽  
Author(s):  
Kristin A. Moore ◽  
Jian Wei Tay ◽  
Jeffrey C. Cameron
Keyword(s):  
Dna Replication ◽  
Cell Growth ◽  
Photosynthetic Bacteria ◽  
Bacterial Species ◽  
Genetic Material ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Life On Earth ◽  
Microbial Dna

ABSTRACTFaithful inheritance of genetic material from one generation to the next is an essential process for all life on earth. Much of what is known about microbial DNA replication and inheritance has been learned from a small number of bacterial species that share many common traits. Whether these pathways are conserved across the great diversity of the microbiome remains unclear. To address this question, we studied chromosome dynamics in a polyploid photosynthetic bacteria using single cell, time-lapse microscopy over multi-generation lineages in conjunction with inducible CRISPR-interference and fluorescent chromosome labeling. With this method we demonstrated the long-term consequences of manipulating parameters such as cell growth, cell division, and DNA replication and segregation on chromosome regulation in a polyploid bacterial species. We find that these bacteria are surprisingly resilient to chromosome disruption resulting in continued cell growth when DNA replication is inhibited and even in the complete absence of chromosomes.

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