scholarly journals Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

10.3791/4319 ◽  
2012 ◽  
Author(s):  
Anastacia M. Garcia ◽  
Mary L. Ladage ◽  
Pamela A. Padilla
Keyword(s):  
Time Lapse ◽  
Suspended Animation ◽  
C Elegans ◽  
Time Lapse Microscopy ◽  
Use Of Time

scholarly journals Use of time-lapse microscopy to visualize rapid movement of the replication origin region of the chromosome during the cell cycle in Bacillus subtilis

1998 ◽  
Vol 28 (5) ◽  
pp. 883-892 ◽  
Author(s):  
Chris D. Webb ◽  
Peter L. Graumann ◽  
Jason A. Kahana ◽  
Aurelio A. Teleman ◽  
Pamela A. Silver ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bacillus Subtilis ◽  
Replication Origin ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Rapid Movement ◽  
Use Of Time ◽  
Origin Region

scholarly journals Time-Lapse Microscopy of Streptomyces coelicolor Growth and Sporulation

2008 ◽  
Vol 74 (21) ◽  
pp. 6774-6781 ◽  
Author(s):  
Vinod Jyothikumar ◽  
Emma J. Tilley ◽  
Rashmi Wali ◽  
Paul R. Herron
Keyword(s):  
Protein Trafficking ◽  
Growth And Development ◽  
Fluorescent Protein ◽  
Imaging System ◽  
Streptomyces Coelicolor ◽  
Time Lapse ◽  
Time Lapse Microscopy ◽  
Use Of Time ◽  
Aerial Hyphae ◽  
Green Fluorescent

ABSTRACT Bacteria from the genus Streptomyces are among the most complex of all prokaryotes; not only do they grow as a complex mycelium, they also differentiate to form aerial hyphae before developing further to form spore chains. This developmental heterogeneity of streptomycete microcolonies makes studying the dynamic processes that contribute to growth and development a challenging procedure. As a result, in order to study the mechanisms that underpin streptomycete growth, we have developed a system for studying hyphal extension, protein trafficking, and sporulation by time-lapse microscopy. Through the use of time-lapse microscopy we have demonstrated that Streptomyces coelicolor germ tubes undergo a temporary arrest in their growth when in close proximity to sibling extension sites. Following germination, in this system, hyphae extended at a rate of ∼20 μm h−1, which was not significantly different from the rate at which the apical ring of the cytokinetic protein FtsZ progressed along extending hyphae through a spiraling movement. Although we were able to generate movies for streptomycete sporulation, we were unable to do so for either the erection of aerial hyphae or the early stages of sporulation. Despite this, it was possible to demonstrate an arrest of aerial hyphal development that we suggest is through the depolymerization of FtsZ-enhanced green fluorescent protein (GFP). Consequently, the imaging system reported here provides a system that allows the dynamic movement of GFP-tagged proteins involved in growth and development of S. coelicolor to be tracked and their role in cytokinesis to be characterized during the streptomycete life cycle.

scholarly journals Long-term time-lapse microscopy of C. elegans post-embryonic development

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Nicola Gritti ◽  
Simone Kienle ◽  
Olga Filina ◽  
Jeroen Sebastiaan van Zon
Keyword(s):  
Embryonic Development ◽  
Time Lapse ◽  
C Elegans ◽  
Time Lapse Microscopy

scholarly journals THE ASSOCIATION OF A CLASS OF SALTATORY MOVEMENTS WITH MICROTUBULES IN CULTURED CELLS

1970 ◽  
Vol 45 (2) ◽  
pp. 334-354 ◽  
Author(s):  
Jerome J. Freed ◽  
Marcia M. Lebowitz
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Phase Contrast ◽  
Cultured Cells ◽  
Time Lapse ◽  
Carbon Particles ◽  
Time Lapse Microscopy ◽  
Use Of Time ◽  
Cytoplasmic Microtubules ◽  
Histochemical Examination

Particulate structures in the cytoplasm of HeLa and other cultured cells in interphase undergo rapid individual linear displacements (long saltatory movements, LSM). By the use of time-lapse microscopy to locate saltating particles prior to fixation and histochemical examination of the cells, structures of several kinds have been shown to move in this manner. Elements that show LSM include lysosomes, pinosomes, ingested carbon particles, lipoidal granules, and unidentified particles that appear as bright objects in positive phase contrast. The pattern of movement of the particles suggests the presence of linear guiding elements radially disposed from the cytocenter (centriole region). The participation of microtubules in these movements is inferred from the observation that LSM cease after treatment with drugs which depolymerize microtubules, i.e., colchicine, Vinblastine, and podophyllin. The directions of the microtubules in the cytoplasm of HeLa cells found by electron microscopy are consistent with the aster-like configuration predicted from study of LSM. Further support for this arrangement of cytoplasmic microtubules is provided by light microscope observations of colchicine-sensitive radial arrays of acid phosphatase granules in the cytoplasm of some cell lines.

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

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