scholarly journals Erratum: Damage-free vibrational spectroscopy of biological materials in the electron microscope

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Peter Rez ◽  
Toshihiro Aoki ◽  
Katia March ◽  
Dvir Gur ◽  
Ondrej L. Krivanek ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy ◽  
Biological Materials

scholarly journals Damage-free vibrational spectroscopy of biological materials in the electron microscope

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Peter Rez ◽  
Toshihiro Aoki ◽  
Katia March ◽  
Dvir Gur ◽  
Ondrej L. Krivanek ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Radiation Damage ◽  
Vibrational Spectroscopy ◽  
Biological Materials ◽  
High Energy ◽  
Vibrational Modes ◽  
Native State ◽  
Organic Functional Groups ◽  
Electron Energy Loss ◽  
Electron Energy Loss Spectra

Abstract Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that severely accelerate sample degradation. Here this major difficulty is overcome using an ‘aloof’ electron beam, positioned tens of nanometres away from the sample: high-energy excitations are suppressed, while vibrational modes of energies <1 eV can be ‘safely’ investigated. To demonstrate the potential of aloof spectroscopy, we record electron energy loss spectra from biogenic guanine crystals in their native state, resolving their characteristic C–H, N–H and C=O vibrational signatures with no observable radiation damage. The technique opens up the possibility of non-damaging compositional analyses of organic functional groups, including non-crystalline biological materials, at a spatial resolution of ∼10 nm, simultaneously combined with imaging in the electron microscope.

scholarly journals Damage-free Analysis of Biological Materials by Vibrational Spectroscopy in the EM

2020 ◽  
Vol 26 (S2) ◽  
pp. 108-110
Author(s):  
Ondrej Krivanek ◽  
Alice Dohnalkova ◽  
Zdravko Kochovski ◽  
Benedikt Haas ◽  
Johannes Müller ◽  
...  
Keyword(s):  
Vibrational Spectroscopy ◽  
Biological Materials

Whole-Organism Analysis by Vibrational Spectroscopy

2019 ◽  
Vol 12 (1) ◽  
pp. 89-108 ◽  
Author(s):  
Dale Christensen ◽  
Anja Rüther ◽  
Kamila Kochan ◽  
David Pérez-Guaita ◽  
Bayden Wood
Keyword(s):  
Vibrational Spectroscopy ◽  
Biological Materials ◽  
Diagnosis And Treatment ◽  
Spectroscopic Techniques ◽  
New Developments ◽  
Raman Spectroscopic ◽  
Spectroscopic Instrumentation ◽  
Near Future ◽  
Made In

Vibrational spectroscopy has contributed to the understanding of biological materials for many years. As the technology has advanced, the technique has been brought to bear on the analysis of whole organisms. Here, we discuss advanced and recently developed infrared and Raman spectroscopic instrumentation to whole-organism analysis. We highlight many of the recent contributions made in this relatively new area of spectroscopy, particularly addressing organisms associated with disease with emphasis on diagnosis and treatment. The application of vibrational spectroscopic techniques to entire organisms is still in its infancy, but new developments in imaging and chemometric processing will likely expand in the field in the near future.

scholarly journals Single-atom vibrational spectroscopy in the scanning transmission electron microscope

Science ◽  
2020 ◽  
Vol 367 (6482) ◽  
pp. 1124-1127 ◽  
Author(s):  
F. S. Hage ◽  
G. Radtke ◽  
D. M. Kepaptsoglou ◽  
M. Lazzeri ◽  
Q. M. Ramasse
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy ◽  
Materials Science ◽  
Scanning Transmission Electron Microscope ◽  
Atomic Scale ◽  
Single Atom ◽  
Phonon Modes ◽  
Resonant States ◽  
Resolution Electron ◽  
Scanning Transmission

Single-atom impurities and other atomic-scale defects can notably alter the local vibrational responses of solids and, ultimately, their macroscopic properties. Using high-resolution electron energy-loss spectroscopy in the electron microscope, we show that a single substitutional silicon impurity in graphene induces a characteristic, localized modification of the vibrational response. Extensive ab initio calculations reveal that the measured spectroscopic signature arises from defect-induced pseudo-localized phonon modes—that is, resonant states resulting from the hybridization of the defect modes and the bulk continuum—with energies that can be directly matched to the experiments. This finding realizes the promise of vibrational spectroscopy in the electron microscope with single-atom sensitivity and has broad implications across the fields of physics, chemistry, and materials science.

Recent Advances of Vibrational Spectroscopy and Chemometrics for Forensic Biological Analysis

The Analyst ◽  
2021 ◽  
Author(s):  
Ayari Takamura ◽  
Takeaki Ozawa
Keyword(s):  
Vibrational Spectroscopy ◽  
Contextual Information ◽  
Crime Scene ◽  
Biological Materials ◽  
Forensic Investigation ◽  
Biological Analysis ◽  
Recent Advances ◽  
Important Evidence

Biological materials found at a crime scene are crucially important evidence for forensic investigation because they provide contextual information about a crime and can be linked to the donor-individuals through...

Vibrational spectroscopy in the electron microscope

Nature ◽  
2014 ◽  
Vol 514 (7521) ◽  
pp. 209-212 ◽  
Author(s):  
Ondrej L. Krivanek ◽  
Tracy C. Lovejoy ◽  
Niklas Dellby ◽  
Toshihiro Aoki ◽  
R. W. Carpenter ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy
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