Vibrational spectroscopy in the electron microscope

Nature ◽  
2014 ◽  
Vol 514 (7521) ◽  
pp. 209-212 ◽  
Author(s):  
Ondrej L. Krivanek ◽  
Tracy C. Lovejoy ◽  
Niklas Dellby ◽  
Toshihiro Aoki ◽  
R. W. Carpenter ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy

scholarly journals Single-atom vibrational spectroscopy in the scanning transmission electron microscope

Science ◽  
2020 ◽  
Vol 367 (6482) ◽  
pp. 1124-1127 ◽  
Author(s):  
F. S. Hage ◽  
G. Radtke ◽  
D. M. Kepaptsoglou ◽  
M. Lazzeri ◽  
Q. M. Ramasse
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy ◽  
Materials Science ◽  
Scanning Transmission Electron Microscope ◽  
Atomic Scale ◽  
Single Atom ◽  
Phonon Modes ◽  
Resonant States ◽  
Resolution Electron ◽  
Scanning Transmission

Single-atom impurities and other atomic-scale defects can notably alter the local vibrational responses of solids and, ultimately, their macroscopic properties. Using high-resolution electron energy-loss spectroscopy in the electron microscope, we show that a single substitutional silicon impurity in graphene induces a characteristic, localized modification of the vibrational response. Extensive ab initio calculations reveal that the measured spectroscopic signature arises from defect-induced pseudo-localized phonon modes—that is, resonant states resulting from the hybridization of the defect modes and the bulk continuum—with energies that can be directly matched to the experiments. This finding realizes the promise of vibrational spectroscopy in the electron microscope with single-atom sensitivity and has broad implications across the fields of physics, chemistry, and materials science.

scholarly journals Damage-free vibrational spectroscopy of biological materials in the electron microscope

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Peter Rez ◽  
Toshihiro Aoki ◽  
Katia March ◽  
Dvir Gur ◽  
Ondrej L. Krivanek ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Radiation Damage ◽  
Vibrational Spectroscopy ◽  
Biological Materials ◽  
High Energy ◽  
Vibrational Modes ◽  
Native State ◽  
Organic Functional Groups ◽  
Electron Energy Loss ◽  
Electron Energy Loss Spectra

Abstract Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that severely accelerate sample degradation. Here this major difficulty is overcome using an ‘aloof’ electron beam, positioned tens of nanometres away from the sample: high-energy excitations are suppressed, while vibrational modes of energies <1 eV can be ‘safely’ investigated. To demonstrate the potential of aloof spectroscopy, we record electron energy loss spectra from biogenic guanine crystals in their native state, resolving their characteristic C–H, N–H and C=O vibrational signatures with no observable radiation damage. The technique opens up the possibility of non-damaging compositional analyses of organic functional groups, including non-crystalline biological materials, at a spatial resolution of ∼10 nm, simultaneously combined with imaging in the electron microscope.

scholarly journals Erratum: Damage-free vibrational spectroscopy of biological materials in the electron microscope

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Peter Rez ◽  
Toshihiro Aoki ◽  
Katia March ◽  
Dvir Gur ◽  
Ondrej L. Krivanek ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy ◽  
Biological Materials

scholarly journals Vibrational Spectroscopy in the Electron Microscope

2016 ◽  
pp. 805-806
Author(s):  
Ondrej Krivanek ◽  
Toshihiro Aoki ◽  
Philip Batson ◽  
Peter Crozier ◽  
Niklas Dellby ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy

scholarly journals Atomically Resolved Vibrational Spectroscopy in the Electron Microscope

2019 ◽  
Vol 25 (S2) ◽  
pp. 592-593
Author(s):  
Fredrik S. Hage ◽  
Demie Kepaptsoglou ◽  
Quentin M. Ramasse ◽  
Leslie J. Allen
Keyword(s):  
Electron Microscope ◽  
Vibrational Spectroscopy

Electron Cytochemical Study of Carbon Dioxide Laser Effect on Rhesus Monkey Cornea

1974 ◽  
Vol 32 ◽  
pp. 224-225
Author(s):  
K. C. Tsou ◽  
J. Morris ◽  
P. Shawaluk ◽  
B. Stuck ◽  
E. Beatrice
Keyword(s):  
Carbon Dioxide ◽  
Electron Microscope ◽  
Rhesus Monkey ◽  
Carbon Dioxide Laser ◽  
Cytochemical Study ◽  
Laser Effect

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

Utilizing Dark Field Electron Microscopy to Produce Lantern Slides

1974 ◽  
Vol 32 ◽  
pp. 116-117
Author(s):  
J. N. Meador ◽  
C. N. Sun ◽  
H. J. White
Keyword(s):  
Electron Microscope ◽  
Electron Micrographs ◽  
Dark Field ◽  
Limiting Factor ◽  
Clinical Laboratories ◽  
Field Electron ◽  
Time Element ◽  
Field Electron Microscopy ◽  
Dark Field Electron ◽  
Dark Field Micrographs

The electron microscope is being utilized more and more in clinical laboratories for pathologic diagnosis. One of the major problems in the utilization of the electron microscope for diagnostic purposes is the time element involved. Recent experimentation with rapid embedding has shown that this long phase of the process can be greatly shortened. In rush cases the making of projection slides can be eliminated by taking dark field electron micrographs which show up as a positive ready for use. The major limiting factor for use of dark field micrographs is resolution. However, for conference purposes electron micrographs are usually taken at 2.500X to 8.000X. At these low magnifications the resolution obtained is quite acceptable.

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