scholarly journals Characterization of glomerular diseases using proteomic analysis of laser capture microdissected glomeruli

2012 ◽  
Vol 25 (5) ◽  
pp. 709-721 ◽  
Author(s):  
Anjali A Satoskar ◽  
John P Shapiro ◽  
Cherri N Bott ◽  
Huijuan Song ◽  
Gyongyi M Nadasdy ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Glomerular Diseases ◽  
Laser Capture ◽  
Laser Capture Microdissected

Proteomic analysis of immunostained, laser-capture microdissected brain samples

2003 ◽  
Vol 24 (12) ◽  
pp. 296-302 ◽  
Author(s):  
Lionel Moulédous ◽  
Sybille Hunt ◽  
Rebecca Harcourt ◽  
Jenny L. Harry ◽  
Keith L. Williams ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Laser Capture ◽  
Laser Capture Microdissected

scholarly journals Spatial gene expression maps of the intestinal lymphoid follicle and associated epithelium identify zonated expression programs

PLoS Biology ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. e3001214
Author(s):  
Noam Cohen ◽  
Hassan Massalha ◽  
Shani Ben-Moshe ◽  
Adi Egozi ◽  
Milena Rozenberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Single Molecule ◽  
Mesenchymal Cell ◽  
Lymphoid Follicle ◽  
Lymphoid Follicles ◽  
Mouse Small Intestine ◽  
Laser Capture ◽  
Laser Capture Microdissected

The intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related to the scarcity and small sizes of ILFs and their follicle-associated epithelium (FAE) impeded the characterization of their spatial gene expression programs. Here, we combined RNA sequencing of laser capture microdissected tissues with single-molecule transcript imaging to obtain a spatial gene expression map of the ILF and its associated FAE in the mouse small intestine. We identified zonated expression programs in both follicles and FAEs, with a decrease in enterocyte antimicrobial and absorption programs and a partial induction of expression programs normally observed at the villus tip. We further identified Lepr+ subepithelial telocytes at the FAE top, which are distinct from villus tip Lgr5+ telocytes. Our analysis exposes the epithelial and mesenchymal cell states associated with ILFs.

scholarly journals Proteomic analysis of laser capture microdissected focal lesions in a rat model of progenitor marker-positive hepatocellular carcinoma

Oncotarget ◽  
2017 ◽  
Vol 8 (16) ◽  
pp. 26041-26056 ◽  
Author(s):  
Adeola O. Adebayo Michael ◽  
Nagib Ahsan ◽  
Valerie Zabala ◽  
Heather Francois-Vaughan ◽  
Stephanie Post ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Rat Model ◽  
Proteomic Analysis ◽  
Focal Lesions ◽  
Laser Capture ◽  
Laser Capture Microdissected

Proteomic analysis of laser capture microdissected human prostate cancer andin vitro prostate cell lines

2000 ◽  
Vol 21 (11) ◽  
pp. 2235-2242 ◽  
Author(s):  
David K. Ornstein ◽  
John W. Gillespie ◽  
Cloud P. Paweletz ◽  
Paul H. Duray ◽  
Judi Herring ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Lines ◽  
Proteomic Analysis ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cell ◽  
Laser Capture ◽  
Prostate Cell Lines ◽  
Laser Capture Microdissected

scholarly journals Spatial expression programs of the intestinal follicle-associated epithelium

2021 ◽  
Author(s):  
Noam Cohen ◽  
Hassan Massalha ◽  
Shani Ben-Moshe ◽  
Adi Egozi ◽  
Milena Rozenberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Single Molecule ◽  
Mesenchymal Cell ◽  
Lymphoid Follicles ◽  
Mouse Small Intestine ◽  
Follicle Associated Epithelium ◽  
Laser Capture ◽  
Laser Capture Microdissected

AbstractThe intestine is lined with isolated lymphoid follicles (ILFs) that facilitate sampling of luminal antigens to elicit immune responses. Technical challenges related to the scarcity and small sizes of ILFs and their follicle-associated epithelium (FAE) impeded the characterization of their spatial gene expression programs. Here, we combined RNA sequencing of laser capture microdissected tissues with single molecule transcript imaging to obtain a spatial gene expression map of the ILF and its associated FAE in the mouse small intestine. We identified zonated expression programs in both follicles and FAEs, with a decrease in enterocyte anti-microbial and absorption programs and a partial induction of expression programs normally observed at the villus tip. We further identified Lepr+ sub-epithelial telocytes at the FAE top, which are distinct from villus-tip Lgr5+ telocytes. Our analysis exposes the epithelial and mesenchymal cell states associated with ILFs.

scholarly journals Characterization of Storage Proteins in Wild (Glycine soja) and Cultivated (Glycine max) Soybean Seeds Using Proteomic Analysis

2006 ◽  
Vol 54 (8) ◽  
pp. 3114-3120 ◽  
Author(s):  
Savithiry S. Natarajan ◽  
Chenping Xu ◽  
Hanhong Bae ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett
Keyword(s):  
Glycine Max ◽  
Proteomic Analysis ◽  
Storage Proteins ◽  
Glycine Soja ◽  
Soybean Seeds
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