scholarly journals Characterization of Storage Proteins in Wild (Glycine soja) and Cultivated (Glycine max) Soybean Seeds Using Proteomic Analysis

2006 ◽  
Vol 54 (8) ◽  
pp. 3114-3120 ◽  
Author(s):  
Savithiry S. Natarajan ◽  
Chenping Xu ◽  
Hanhong Bae ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett
Keyword(s):  
Glycine Max ◽  
Proteomic Analysis ◽  
Storage Proteins ◽  
Glycine Soja ◽  
Soybean Seeds

scholarly journals Genome-wide identification and characterization of InDels and SNPs in Glycine max and Glycine soja for contrasting seed permeability traits

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
G. Ramakrishna ◽  
Parampreet Kaur ◽  
Deepti Nigam ◽  
Pavan K. Chaduvula ◽  
Sangita Yadav ◽  
...  
Keyword(s):  
Glycine Max ◽  
Glycine Soja ◽  
Genome Wide ◽  
Identification And Characterization

scholarly journals Characterization of Vascular plant One-Zinc finger (VOZ) in soybean (Glycine max and Glycine soja) and their expression analyses under drought condition

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0253836
Author(s):  
Shoaib Ur Rehman ◽  
Ghulam Qanmber ◽  
Muhammad Hammad Nadeem Tahir ◽  
Ahsan Irshad ◽  
Sajid Fiaz ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Glycine Max ◽  
Zinc Finger ◽  
Abiotic Stresses ◽  
Glycine Soja ◽  
Vascular Plant ◽  
Orthologous Gene ◽  
Amino Acid Residues ◽  
Sequence Logos

Vascular plant one-zinc-finger (VOZ) transcription factors regulate plant growth and development under drought conditions. Six VOZ transcription factors encoding genes exist in soybean genome (both in Glycine max and Glycine soja). Herein, GmVOZs and GsVOZs were identified through in silico analysis and characterized with different bioinformatics tools and expression analysis. Phylogenetic analysis classified VOZ genes in four groups. Sequence logos analysis among G. max and G. soja amino acid residues revealed higher conservation. Presence of stress related cis-elements in the upstream regions of GmVOZs and GsVOZs highlights their role in tolerance against abiotic stresses. The collinearity analysis identified 14 paralogous/orthologous gene pairs within and between G. max and G. soja. The Ka/Ks values showed that soybean VOZ genes underwent selection pressure with limited functional deviation arising from whole genome and segmental duplication. The GmVOZs and GsVOZs were found to express in roots and leaves at seedling stage. The qRT-PCR revealed that GmVOZs and GsVOZs transcripts can be regulated by abiotic stresses such as polyethylene glycol (PEG). The findings of this study will provide a reference to decipher physiological and molecular functions of VOZ genes in soybean.

REGIONAL CHARACTERIZATION OF TOCOPHEROLS AND DISTRIBUTION OF FATTY ACIDS WITHIN SOYBEAN SEEDS (GLYCINE MAX L.)

2006 ◽  
Vol 13 (1) ◽  
pp. 12-26 ◽  
Author(s):  
HIROMI YOSHIDA ◽  
SAKI KANREI ◽  
YUKA TOMIYAMA ◽  
YOSHIYUKI MIZUSHINA
Keyword(s):  
Fatty Acids ◽  
Glycine Max ◽  
Soybean Seeds ◽  
Glycine Max L

Cloning and Characterization of GmTINY1 Gene in Soybean (Glycine max)

2009 ◽  
Vol 35 (12) ◽  
pp. 2174-2179 ◽  
Author(s):  
Fang HUANG ◽  
Hui HE ◽  
Ying-Jun CHI ◽  
Jun-Yi GAI ◽  
De-Yue YU
Keyword(s):  
Glycine Max ◽  
I Gene

scholarly journals Bacterial inducible expression of plant cell wall-binding protein YesO through conflict between Glycine max and saprophytic Bacillus subtilis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Haruka Sugiura ◽  
Ayumi Nagase ◽  
Sayoko Oiki ◽  
Bunzo Mikami ◽  
Daisuke Watanabe ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Glycine Max ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Binding Protein ◽  
Fermented Food ◽  
Physiological Status ◽  
Soybean Seeds ◽  
Initial Growth

Abstract Saprophytic bacteria and plants compete for limited nutrient sources. Bacillus subtilis grows well on steamed soybeans Glycine max to produce the fermented food, natto. Here we focus on bacterial responses in conflict between B. subtilis and G. max. B. subtilis cells maintained high growth rates specifically on non-germinating, dead soybean seeds. On the other hand, viable soybean seeds with germinating capability attenuated the initial growth of B. subtilis. Thus, B. subtilis cells may trigger saprophytic growth in response to the physiological status of G. max. Scanning electron microscope observation indicated that B. subtilis cells on steamed soybeans undergo morphological changes to form apertures, demonstrating cell remodeling during saprophytic growth. Further, transcriptomic analysis of B. subtilis revealed upregulation of the gene cluster, yesOPQR, in colonies growing on steamed soybeans. Recombinant YesO protein, a putative, solute-binding protein for the ATP-binding cassette transporter system, exhibited an affinity for pectin-derived oligosaccharide from plant cell wall. The crystal structure of YesO, in complex with the pectin oligosaccharide, was determined at 1.58 Å resolution. This study expands our knowledge of defensive and offensive strategies in interspecies competition, which may be promising targets for crop protection and fermented food production.

Three dimensional characterization of micronized soybean seeds using X-ray microtomography

2021 ◽  
Vol 127 ◽  
pp. 388-397
Author(s):  
Rani Puthukulangara Ramachandran ◽  
Chyngyz Erkinbaev ◽  
Sandeep Thakur ◽  
Jitendra Paliwal
Keyword(s):  
Three Dimensional ◽  
Soybean Seeds ◽  
X Ray

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

scholarly journals The Nutritional Value and Safety of Genetically Unmodified Soybeans and Soybean Feed Products in the Nutrition of Farm Animals

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1105
Author(s):  
Małgorzata Świątkiewicz ◽  
Kamil Witaszek ◽  
Ewa Sosin ◽  
Krzysztof Pilarski ◽  
Beata Szymczyk ◽  
...  
Keyword(s):  
Glycine Max ◽  
Nutritional Value ◽  
Urease Activity ◽  
Basic Protein ◽  
Press Cake ◽  
Farm Animals ◽  
Soybean Seeds ◽  
Lower Content ◽  
Glycine Max L ◽  
Protein Feed

Post-extraction soybean (Glycine max (L.) Merr.) meal is widely used as a basic protein feed for farm animals, especially poultry and pigs. Products made from unmodified soybean seeds are an alternative to imported GMO soybean meal. The aim of the study was to develop feed products from popular European varieties of genetically unmodified soybeans, which can be produced on small and medium-sized farms, and to assess their nutritional value and safety to livestock. The research was conducted on the seeds of three soybean varieties and two types of feed products resulting from thermobaric treatment (extrudate) and oil pressing (soybean press cake). The mould and yeast contamination of domestic seeds was negligible. The thermobaric and pressing treatments lowered the content of fungi by 97%. The products were considered free from mycotoxins. In comparison with full-fat soybean seeds, the protein content in the products was up to 19% higher, and 92% of the total lysine remained available. The products had lower content of antinutritional ingredients (trypsin inhibitors) and the urease activity was reduced by 52–59% and 99%, respectively. The experiment showed that the European genetically unmodified soybean feed products were characterised by good quality, mycotoxicological purity and high nutritional value for farm animals.

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