scholarly journals In Vivo Imaging of Neocortical and Hippocampal CA1 Neurons by Two-photon Microscopy

2014 ◽  
Vol 54 (1) ◽  
pp. 035-038
Author(s):  
Ryosuke KAWAKAMI ◽  
Terumasa HIBI ◽  
Tomomi NEMOTO
Keyword(s):  
In Vivo Imaging ◽  
Ca1 Neurons ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Hippocampal Ca1 Neurons ◽  
Hippocampal Ca1

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 3303 ◽  
Author(s):  
Seoyeon Bok ◽  
Taejun Wang ◽  
Chan-Ju Lee ◽  
Seong-Uk Jeon ◽  
Young-Eun Kim ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Mouse Model ◽  
In Vivo Imaging ◽  
Focal Cerebral Ischemia ◽  
Activated Microglia ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Optical clearing of living brains with MAGICAL to extend in vivo imaging

2019 ◽  
Author(s):  
Kouichirou Iijima ◽  
Takuto Oshima ◽  
Ryosuke Kawakami ◽  
Tomomi Nemoto
Keyword(s):  
Fluorescence Emission ◽  
Cortical Layer ◽  
Fluorescence Emission Spectrum ◽  
Spatiotemporal Resolution ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Brain Functions ◽  
Hippocampal Ca1 ◽  
Layer V

AbstractTo understand brain functions, it is important to observe directly how multiple neural circuits are performing in living brains. However, due to tissue opaqueness, observable depth and spatiotemporal resolution are severely degraded in vivo. Here, we propose an optical brain clearing method for in vivo fluorescence microscopy, termed MAGICAL (Magical Additive Glycerol Improves Clear Alive Luminance). MAGICAL enabled two-photon microscopy to capture vivid images with fast speed, at cortical layer V and hippocampal CA1 in vivo. Moreover, MAGICAL promoted conventional confocal microscopy to visualize finer neuronal structures including synaptic boutons and spines in unprecedented deep regions, without intensive illumination leading to phototoxic effects. Fluorescence Emission Spectrum Transmissive Analysis (FESTA) showed that MAGICAL improved in vivo transmittance of shorter wavelength light, which is vulnerable to optical scattering thus unsuited for in vivo microscopy. These results suggest that MAGICAL would transparentize living brains via scattering reduction.

Photostable far-red emitting pluronic silicate nanoparticles: perfect blood pool fluorophores for biphotonic in vivo imaging of the leaky tumour vasculature

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 94200-94205 ◽  
Author(s):  
Zheng Zheng ◽  
Flavien Caraguel ◽  
Yuan-Yuan Liao ◽  
Chantal Andraud ◽  
Boudewijn van der Sanden ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Blood Pool ◽  
Pluronic F127 ◽  
Photon Absorption ◽  
Absorption Properties ◽  
Two Photon Absorption ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Tumour Vasculature

A new non-diffusible fluorescent probe for two photon microscopy, comprising a hydrophobic push-pull dye in the apolar core of Pluronic F127–silica nanoparticles, shows intense red emission (Φf 39% at 650 nm) and two-photon absorption properties in the NIR.

Upconverting nanoparticles: a versatile platform for wide-field two-photon microscopy and multi-modal in vivo imaging

2015 ◽  
Vol 44 (6) ◽  
pp. 1302-1317 ◽  
Author(s):  
Yong Il Park ◽  
Kang Taek Lee ◽  
Yung Doug Suh ◽  
Taeghwan Hyeon
Keyword(s):  
In Vivo Imaging ◽  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon

Upconverting nanoparticles (UCNPs) enable the establishment of a novel UCNP-based platform for wide-field two-photon microscopy and multimodal in vivo imaging.

scholarly journals In Vivo Imaging of T-Cell Motility in the Elicitation Phase of Contact Hypersensitivity Using Two-Photon Microscopy

2011 ◽  
Vol 131 (4) ◽  
pp. 977-979 ◽  
Author(s):  
Gyohei Egawa ◽  
Tetsuya Honda ◽  
Hideaki Tanizaki ◽  
Hiromi Doi ◽  
Yoshiki Miyachi ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Motility ◽  
In Vivo Imaging ◽  
Contact Hypersensitivity ◽  
Two Photon Microscopy ◽  
Two Photon

Adaptive optics two-photon microscopy for in vivo imaging of mouse retina

2020 ◽  
Author(s):  
Zhongya Qin ◽  
Sicong He ◽  
Congping Chen ◽  
Chao Yang ◽  
Jasmine Yung ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Mouse Retina ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Application of multiline two-photon microscopy to functional in vivo imaging

2006 ◽  
Vol 151 (2) ◽  
pp. 276-286 ◽  
Author(s):  
Rafael Kurtz ◽  
Matthias Fricke ◽  
Julia Kalb ◽  
Philip Tinnefeld ◽  
Markus Sauer
Keyword(s):  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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