scholarly journals In situ hybridization studies of matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-1 and type IV collagen in diabetic nephropathy

1997 ◽  
Vol 52 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Daisuke Suzuki ◽  
Masanobu Miyazaki ◽  
Kiichiro Jinde ◽  
Takehiko Koji ◽  
Mitsunori Yagame ◽  
...  
2001 ◽  
Vol 21 (6) ◽  
pp. 445-447 ◽  
Author(s):  
Tsukasa Nakamura ◽  
Chifuyu Ushiyama ◽  
Noriaki Shimada ◽  
Kayo Hayashi ◽  
Isao Ebihara ◽  
...  

1989 ◽  
Vol 27 ◽  
pp. 85
Author(s):  
P. Simon-Assmann ◽  
F. Perrin-Schmitt ◽  
F. Bouziges ◽  
M. Kedinger

1990 ◽  
Vol 110 (3) ◽  
pp. 849-857 ◽  
Author(s):  
P Simon-Assmann ◽  
F Bouziges ◽  
J N Freund ◽  
F Perrin-Schmitt ◽  
M Kedinger

The expression of type IV collagen mRNA during mouse intestinal morphogenesis was examined by in situ hybridization using a cDNA probe corresponding to mRNA for alpha 1 (IV) chain. Type IV collagen mRNA is detected in the embryonic mesenchymal cells at early stages of development (12 d of gestation). A segregation of mesenchymal cells expressing high levels of type IV collagen mRNA in close vicinity of the epithelium occurs just before villus formation. During villus outgrowth, type IV collagen mRNA, still confined to mesenchyme-derived tissues, is progressively restricted to the mucosal connective tissue (the lamina propria) and to a lesser extent to the muscular layers. In the adult, the amount of messenger is quite low as compared to the level found in the developing intestine and the in situ hybridization signal, indistinguishable from the background, is uniform throughout the whole intestinal wall. At all developmental stages no detectable specific hybridization signal is virtually observed over the epithelium cell layer. These results show that high amounts of the type IV collagen messenger are detected during phases of intensive morphogenetic events. Furthermore, they reinforce the notion already gained previously (Simon-Assmann et al. 1988) that the mesenchymal compartment is the principal endogenous source of type IV collagen. They also indicate that the continuous migration of epithelial cells along the basement membrane of intestinal villi in the mature organ is not accompanied by a significant remodeling of the collagen IV network.


1994 ◽  
Vol 174 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Mohammed S. Razzaque ◽  
Takehiko Koji ◽  
Takashi Taguchi ◽  
Takashi Harada ◽  
Paul K. Nakane

2005 ◽  
Vol 22 (1) ◽  
pp. 039-044 ◽  
Author(s):  
Faruk Tas ◽  
Derya Duranyildiz ◽  
Hilal Oguz ◽  
Rian Disci ◽  
Sidika Kurul ◽  
...  

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