scholarly journals Insulin-like growth factor-binding protein 1 and 7 concentrations are lower in obese pregnant women, women with gestational diabetes and their fetuses

2014 ◽  
Vol 35 (1) ◽  
pp. 32-38 ◽  
Author(s):  
M Lappas
Keyword(s):  
Growth Factor ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding

Serum Insulin-Like Growth Factor Binding Protein-1 in Pregnant Women: Decreased Concentrations following an Oral Glucose Load

1994 ◽  
Vol 31 (1) ◽  
pp. 40-45 ◽  
Author(s):  
P C O'Leary ◽  
M Longley
Keyword(s):  
Growth Factor ◽  
Pregnant Women ◽  
Binding Protein ◽  
Serum Insulin ◽  
Oral Glucose ◽  
Insulin Like Growth Factor ◽  
Oral Glucose Load ◽  
Glucose Load ◽  
Factor Binding ◽  
Igf I

Serum concentrations of insulin-like growth factor binding protein-1 (IGFBP-1) were measured in men and in non-pregnant and pregnant women. Peak serum IGFBP-1 levels occurred mid-gestation followed by a slight decline towards term. Women with gestational diabetes had higher serum IGFBP-1 concentrations than apparently healthy women at comparable gestation. Following administration of an oral glucose load, serum IGFBP-1 concentrations were decreased within 2 h in men and in pregnant women while IGF-I levels remained constant. These results suggested that IGFBP-1 regulates IGF-I activity in pregnancy in a similar manner to that in the non-pregnant state.

Purification and assay of insulin-like growth factor-binding protein-1: measurement of circulating levels throughout pregnancy

1991 ◽  
Vol 128 (1) ◽  
pp. 161-168 ◽  
Author(s):  
H. S. Wang ◽  
L. A. Perry ◽  
J. Kanisius ◽  
R. K. Iles ◽  
J. M. P. Holly ◽  
...  
Keyword(s):  
Growth Factor ◽  
Pregnant Women ◽  
Binding Protein ◽  
Gel Filtration ◽  
Serum Levels ◽  
First Trimester ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Reducing Conditions ◽  
Sds Page

ABSTRACT Insulin-like growth factor-binding protein-1 (IGFBP-1) has been purified from amniotic fluid by anion exchange, hydrophobic interaction and gel filtration chromatography. The overall recovery of the purification process was 12·2%. The purified IGFBP-1 yielded a single band on SDS-PAGE gel but showed two bands (34 kDa and 68 kDa) on Western blot under non-reducing conditions. Polyclonal antisera were raised by immunization of sheep using the purified IGFBP-1. The best antiserum bound 50% of 125I-labelled IGFBP-1 at a final dilution of 1:500 000. A radioimmunoassay for IGFBP-1 was developed. This assay had a minimum detection limit of 5 μg/1, and was used to determine serum levels in non-pregnant and pregnant women. There was no cross-reaction with a wide variety of materials tested. Serum IGFBP-1 levels in non-pregnant individuals (33±16 (s.D.) μg/1) were found to be significantly lower than those in the second (96±64 μg/1) and third trimesters (95 ±60 μg/1) of pregnant women. During pregnancy, circulating IGFBP-1 levels increased rapidly in the first trimester and reached a peak at 12–13 weeks of gestation (107±75 μg/l). The level then remained at 80±53 to 103±70 μg/l until term. Journal of Endocrinology (1991) 128, 161–168

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