Dendritic cells expressing soluble CTLA4Ig prolong antigen‐specific skin graft survival

2010 ◽  
Vol 88 (8) ◽  
pp. 846-850 ◽  
Author(s):  
Francesco L Ierino ◽  
William Mulley ◽  
Natalie Dodge ◽  
Yu Qin Li ◽  
Effie Mouhtouris ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Graft Survival ◽  
Skin Graft

scholarly journals Concurrent CCR7 Overexpression and RelB Knockdown in Immature Dendritic Cells Induces Immune Tolerance and Improves Skin-Graft Survival in a Murine Model

2017 ◽  
Vol 42 (2) ◽  
pp. 455-468 ◽  
Author(s):  
Zhiwei Dong ◽  
Yajie Chen ◽  
Yuan Peng ◽  
Fan Wang ◽  
Zichen Yang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Graft Survival ◽  
Skin Graft ◽  
Immune Tolerance ◽  
Migration Rate ◽  
Recombinant Adenovirus ◽  
Treg Cells ◽  
Skin Transplantation ◽  
Immune Rejection ◽  
Immature Dendritic Cells

Background/Aims: Skin transplantation aims to cover skin defects but often fails due to immune rejection of the transplantated tissue. Immature dendritic cells (imDCs) induce immune tolerance but have a low migration rate. After stimulation, imDCs transform into mature DCs, which activate immune rejection. Thus, inducing imDC to obtain a high migration counteracts development of immune tolerance. Methods & Results: We transfected imDCs with a recombinant adenovirus carrying the CCR7 gene (Ad-CCR7) and a small interfering RNA targeting RelB (RelB-siRNA) to concurrently overexpress CCR7 and downregulate RelB expression. Functionally, such cells showed a significantly enhanced migration rate in the chemotactic assay and decreased T-cell proliferation after lipopolysaccharide stimulation in mixed lymphocyte reactions. Cotransfected cells showed an increased ability to induce immune tolerance by upregulating T regulatory (Treg) cells and shifting the Th1/Th2 ratio. Cotransfection of Ad-CCR7 and RelB-siRNA endowed imDCs with resistance to apoptosis and cell death. CCR7 overexpression and RelB knockdown (KD) in imDCs improve skin-graft survival in a murine skin-transplantation model. Conclusion: Transfection with Ad-CCR7 and RelB KD in imDCs may be an effective approach inducing immune tolerance, thus being potentially valuable for inhibiting allograft rejection.

MURINE DENDRITIC CELLS (JAWS II) EXPRESSING SOLUBLE CTLA4IG PROLONGS FIRST AND SECOND SET SKIN GRAFT SURVIVAL

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 508
Author(s):  
F Ierino ◽  
W Mulley ◽  
N Dodge ◽  
Y Q. Li ◽  
D Christiansen ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Graft Survival ◽  
Skin Graft

Mesenchymal stem cells increase skin graft survival time and up-regulate PD-L1 expression in splenocytes of mice

2017 ◽  
Vol 182 ◽  
pp. 39-49 ◽  
Author(s):  
Ali Moravej ◽  
Bita Geramizadeh ◽  
Negar Azarpira ◽  
Amir-Hassan Zarnani ◽  
Ramin Yaghobi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Survival Time ◽  
Graft Survival ◽  
Skin Graft

Corrigendum to “Role of serum amyloid P in skin graft survival and wound healing in burned patients receiving skin grafts” [Clinica Chimica Acta 412 (2011) 227-229]

2011 ◽  
Vol 412 (7-8) ◽  
pp. 670
Author(s):  
Yi-ming Zhang ◽  
Ya-dong Fang ◽  
Yi-cheng Wang ◽  
Shao-liang Wang ◽  
Ze-yuan Lei ◽  
...  
Keyword(s):  
Wound Healing ◽  
Graft Survival ◽  
Skin Graft ◽  
Serum Amyloid ◽  
Skin Grafts ◽  
Serum Amyloid P ◽  
Amyloid P
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