Prolongation of skin graft survival in mice by in vitro PUVA treatment and failure of induction of specific immunological memory by PUVA-treated grafts

1984 ◽  
Vol 276 (2) ◽  
pp. 82-85 ◽  
Author(s):  
S. Gruner ◽  
H. Meffert ◽  
E. Karasek ◽  
N. S�nnichsen
Keyword(s):  
Graft Survival ◽  
Skin Graft ◽  
Immunological Memory ◽  
Puva Treatment ◽  
Failure Of Induction

scholarly journals Treg-mediated prolonged survival of skin allografts without immunosuppression

2019 ◽  
Vol 116 (27) ◽  
pp. 13508-13516 ◽  
Author(s):  
Nina Pilat ◽  
Mario Wiletel ◽  
Anna M. Weijler ◽  
Romy Steiner ◽  
Benedikt Mahr ◽  
...  
Keyword(s):  
Graft Survival ◽  
Skin Graft ◽  
Interleukin 2 ◽  
Tolerance Induction ◽  
Allograft Survival ◽  
Skin Allograft ◽  
Skin Allografts

Injection of Interleukin-2 (IL-2) complexed with a particular anti–IL-2 monoclonal antibody (mab) JES6-1 has been shown to selectively expand CD4+Foxp3+ T regulatory T cells (Tregs) in vivo. Although the potency of this approach with regard to transplantation has already been proven in an islet transplantation model, skin graft survival could not be prolonged. Since the latter is relevant to human allograft survival, we sought to improve the efficiency of IL-2 complex (cplx) treatment for skin allograft survival in a stringent murine skin graft model. Here, we show that combining low doses of IL-2 cplxs with rapamycin and blockade of the inflammatory cytokine IL-6 leads to long-term (>75 d) survival of major histocompatibility complex-different skin allografts without the need for immunosuppression. Allograft survival was critically dependent on CD25+FoxP3+ Tregs and was not accompanied by impaired responsiveness toward donor alloantigens in vitro after IL-2 cplx treatment was stopped. Furthermore, second donor-type skin grafts were rejected and provoked rejection of the primary graft, suggesting that operational tolerance is not systemic but restricted to the graft. These findings plus the lack of donor-specific antibody formation imply that prolonged graft survival was largely a reflection of immunological ignorance. The results may represent a potentially clinically translatable strategy for the development of protocols for tolerance induction.

Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo

2002 ◽  
Vol 30 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Amelia Bartholomew ◽  
Cord Sturgeon ◽  
Mandy Siatskas ◽  
Karen Ferrer ◽  
Kevin McIntosh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graft Survival ◽  
Skin Graft ◽  
Lymphocyte Proliferation ◽  
Proliferation In Vitro

A Systematic Review on Extracellular Vesicles-Enriched Fat Grafting: A Shifting Paradigm

2020 ◽  
Author(s):  
Mohammad Ghiasloo ◽  
Laura De Wilde ◽  
Kashika Singh ◽  
Patrick Tonnard ◽  
Alexis Verpaele ◽  
...  
Keyword(s):  
Systematic Review ◽  
Graft Survival ◽  
Extracellular Vesicles ◽  
Retention Rate ◽  
Fat Grafting ◽  
Retention Rates ◽  
Fat Graft ◽  
Cochrane Central Register

Abstract Background Recent evidence confirms that mesenchymal stem cells (MSCs) facilitate angiogenesis mainly through paracrine function. Extracellular vesicles (EVs) are regarded as key components of the cell secretome, possessing functional properties of their source cells. Subsequently, MSC-EVs have emerged as a novel cell-free approach to improve fat graft retention rate. Objectives To provide a systematic review of all studies reporting the use of MSC-EVs to improve graft retention rate. Methods A systematic search was undertaken using the Embase, PubMed and the Cochrane Central Register of Controlled Trials databases. Outcome measures included donor/receptor organism of the fat graft, study model, intervention groups, evaluation intervals, EV research data, in vitro and in vivo results. Results Of the total 1717 articles, 62 full-texts were screened. Seven studies reporting on 294mice were included. Overall, EV treated groups showed higher graft retention rates compared to untreated groups. Notably, retention rate was similar following EV- and MSC-treatment. In addition to reduced inflammation, graft enrichment with EVs resulted in early revascularization and better graft integrity. Interestingly, hypoxic preconditioning of MSCs improved their beneficial paracrine effects and led to a more proangiogenic EV population, as observed by both in vitro and in vivo results. Conclusions MSC-EVs appear to offer an interesting cell-free alternative to improve fat graft survival. While their clinical relevance remains to be determined, it is clear that not the cells, but their secretome is essential for graft survival. Thus, a paradigm shift from cell-assisted lipotransfer towards ‘secretome-assisted lipotransfer’ is well on its way.

Mesenchymal stem cells increase skin graft survival time and up-regulate PD-L1 expression in splenocytes of mice

2017 ◽  
Vol 182 ◽  
pp. 39-49 ◽  
Author(s):  
Ali Moravej ◽  
Bita Geramizadeh ◽  
Negar Azarpira ◽  
Amir-Hassan Zarnani ◽  
Ramin Yaghobi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Survival Time ◽  
Graft Survival ◽  
Skin Graft

Corrigendum to “Role of serum amyloid P in skin graft survival and wound healing in burned patients receiving skin grafts” [Clinica Chimica Acta 412 (2011) 227-229]

2011 ◽  
Vol 412 (7-8) ◽  
pp. 670
Author(s):  
Yi-ming Zhang ◽  
Ya-dong Fang ◽  
Yi-cheng Wang ◽  
Shao-liang Wang ◽  
Ze-yuan Lei ◽  
...  
Keyword(s):  
Wound Healing ◽  
Graft Survival ◽  
Skin Graft ◽  
Serum Amyloid ◽  
Skin Grafts ◽  
Serum Amyloid P ◽  
Amyloid P

T2 B Cells Are Expanded During Experimental Tolerance Protocol and Contribute to Skin Graft Survival

2012 ◽  
Vol 94 (10S) ◽  
pp. 479
Author(s):  
A. Moreau ◽  
P. Blair ◽  
E. Stolarczyk ◽  
K. Ratnasothy ◽  
R. Alhabbab ◽  
...  
Keyword(s):  
B Cells ◽  
Graft Survival ◽  
Skin Graft

Skin graft survival on a fascia lata graft

1980 ◽  
Vol 33 (2) ◽  
pp. 143-144 ◽  
Author(s):  
M.N. Saad ◽  
C.T.K. Khoo
Keyword(s):  
Graft Survival ◽  
Skin Graft ◽  
Fascia Lata ◽  
Fascia Lata Graft
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