ASPECTS OF STIMULATION OF BONE MARROW COLONY GROWTH IN VITRO

1968 ◽  
Vol 46 (3) ◽  
pp. 335-342 ◽  
Author(s):  
TR Bradley
Keyword(s):  
Bone Marrow ◽  
Colony Growth ◽  
Stimulation Of

scholarly journals Brief Report: Stimulation of Bone Marrow Colony Growth In Vitro by Human Urine

Blood ◽  
1969 ◽  
Vol 33 (3) ◽  
pp. 396-399 ◽  
Author(s):  
W. A. ROBINSON ◽  
E. R. STANLEY ◽  
D. METCALF
Keyword(s):  
Bone Marrow ◽  
Cell Growth ◽  
Mononuclear Cell ◽  
Human Urine ◽  
Colony Growth ◽  
Urine Samples ◽  
Disease States ◽  
Normal Humans ◽  
Stimulation Of

Abstract Using a new technic of bone marrow culture in agar, urine samples from 50 humans have been tested for their ability to stimulate the formation of granulocyte—mononuclear cell growth in vitro. Significant colony stimulating activity has been found with 25 out of 50 unconcentrated urine samples from both normal humans and patients with a variety of disease states.

Thrombopoietin-Induced Stimulation of Megakaryocyte- Enriched Bone Marrow Cultures

1979 ◽  
Author(s):  
K. L. Kellar ◽  
B. L. Evatt ◽  
C. R. McGrath ◽  
R. B. Ramsey
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Rabbit Plasma ◽  
Bone Marrow Cultures ◽  
Plasma Fractions ◽  
Marrow Cultures ◽  
Stimulation Of

Liquid cultures of bone marrow cells enriched for megakaryocytes were assayed for incorporation of 3H-thymidine (3H-TdR) into acid-precipitable cell digests to determine the effect of thrombopoietin on DNA synthesis. As previously described, thrombopoietin was prepared by ammonium sulfate fractionation of pooled plasma obtained from thrombocytopenic rabbits. A control fraction was prepared from normal rabbit plasma. The thrombopoietic activity of these fractions was determined in vivo with normal rabbits as assay animals and the rate of incorporation of 75Se-selenomethionine into newly formed platelets as an index of thrombopoietic activity of the infused material. Guinea pig megakaryocytes were purified using bovine serum albumin gradients. Bone marrow cultures containing 1.5-3.0x104 cells and 31%-71% megakaryocytes were incubated 18 h in modified Dulbecco’s MEM containing 10% of the concentrated plasma fractions from either thrombocytopenic or normal rabbits. In other control cultures, 0.9% NaCl was substituted for the plasma fractions. 3H-TdR incorporation was measured after cells were incubated for 3 h with 1 μCi/ml. The protein fraction containing thrombopoietin-stimulating activity caused a 25%-31% increase in 3H-TdR incorporation over that in cultures which were incubated with the similar fraction from normal plasma and a 29% increase over the activity in control cultures to which 0.9% NaCl had been added. These data suggest that thrombopoietin stimulates DNA synthesis in megakaryocytes and that this tecnique may be useful in assaying thrombopoietin in vitro.

scholarly journals Autosomal dominant polycythemia

Blood ◽  
1985 ◽  
Vol 66 (5) ◽  
pp. 1208-1214 ◽  
Author(s):  
JT Prchal ◽  
WM Crist ◽  
E Goldwasser ◽  
G Perrine ◽  
JF Prchal
Keyword(s):  
Autosomal Dominant ◽  
Oxygen Affinity ◽  
Colony Growth ◽  
Blood Oxygen ◽  
Autosomal Dominant Trait ◽  
Volume Measurements ◽  
Blood Oxygen Affinity ◽  
Low Levels ◽  
Stimulation Of

Two families with polycythemia inherited as an autosomal dominant trait are described. Serial hemoglobin determinations in multiple family members and RBC volume measurements in selected affected subjects documented their polycythemia. Measurements of arterial p02s, p50s, and blood oxygen affinity were normal in all affected individuals from each family who were tested. Erythropoietin (EPO) levels were low in affected individuals from family 1 and normal in affected members of family 2. Stimulation of in vitro CFU-E colony growth by low levels of EPO was significantly increased in subjects from family 1, but normal in those affected from family 2. We conclude that although the inheritance pattern for the polycythemia in both of these families appeared to be the same, the biologic defect leading to the disorder in each of these unique families was different. The precise mechanism of the increased EPO sensitivity noted in affected subjects from family 1 awaits elucidation.

scholarly journals In vitro stimulation of primate hemoglobin synthesis by L-thyroxine

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 407-413 ◽  
Author(s):  
JE Fuhr ◽  
N Gengozian ◽  
M Overton
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Leucine Incorporation ◽  
Hemoglobin Synthesis ◽  
Globin Chains ◽  
Stimulation Of ◽  
Marrow Cells

Abstract Bone marrow cells from adult and abortus primates (marmosets) were incubated in vitro to determine their responsiveness to L-thyroxine. 3H- leucine incorporation into purified globin chains was the parameter assayed to determine responsiveness. Bone marrow from spontaneously aborted animals consistently was stimulated by the presence of physiologic levels of L-thyroxine. Bone marrow cells from adult animals were unaffected by the hormone.

scholarly journals Cytogenetic studies and in vitro colony growth in patients with mastocytosis

Blood ◽  
1987 ◽  
Vol 70 (6) ◽  
pp. 1928-1932 ◽  
Author(s):  
B Swolin ◽  
S Rodjer ◽  
G Roupe
Keyword(s):  
Bone Marrow ◽  
Growth Pattern ◽  
Bone Marrow Cells ◽  
Systemic Mastocytosis ◽  
Colony Growth ◽  
Chromosome Abnormalities ◽  
Control Group ◽  
Normal Peripheral Blood ◽  
Abnormal Growth

Abstract Cytogenetic analysis of bone marrow cells and in vitro growth for bone marrow granulocytic-macrophage stem cells have been performed in 13 patients with mastocytosis, six with systemic mastocytosis, and seven with urticaria pigmentosa. Clones with chromosome abnormalities were found in five patients. The number of clusters and/or colonies after seven days in culture was increased in seven patients, compared with the growth in a control group. Three patients with chromosome abnormalities showed an abnormal growth pattern, yet exhibited normal peripheral blood values. Two patients with systemic mastocytosis had clones with chromosome abnormalities and some abnormal hematological values. The proportion of patients with chromosome abnormalities and an abnormal growth pattern was higher among these patients with mastocytosis than in healthy control subjects. These results may be of interest when discussing the origin of mast cell disorders and indicate an association with the myeloproliferative disorders.

scholarly journals Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes

Blood ◽  
1986 ◽  
Vol 68 (6) ◽  
pp. 1316-1321 ◽  
Author(s):  
WE Fibbe ◽  
J van Damme ◽  
A Billiau ◽  
PJ Voogt ◽  
N Duinkerken ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Interleukin 1 ◽  
Colony Growth ◽  
Cell Suspensions ◽  
Mononuclear Phagocytes ◽  
Adherent Cells ◽  
Macrophage Colony

Abstract An electrophoretically pure preparation of natural human interleukin-1 (IL-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-1, the number of day 4 CFC reached 72%, the number of day 7 CFU-GM reached 32%, and the number of day 10 CFU-GM reached 80% of the respective numbers of colonies obtained by addition of crude leukocyte-conditioned medium (LCM). The IL-1-induced stimulatory effect on CFU-GM growth could be completely neutralized by a rabbit anti-IL-1 antiserum. Colony growth was abrogated by depleting the marrow cell suspensions of phagocytic cells prior to IL-1 addition. Conversely, the effect could be reintroduced by addition of marrow-derived adherent cells to bone marrow cell suspensions that had been depleted of both phagocytic and E rosetting T cells. Furthermore, media conditioned by bone marrow-derived adherent cells or by peripheral blood mononuclear phagocytes in the presence but not in the absence of IL-1, stimulated in vitro colony growth of phagocyte-depleted bone marrow cell suspensions. These results indicate that IL-1 induces release of granulocyte-macrophage colony-stimulating activity (GM-CSA) from human mononuclear phagocytes.

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