COLONY GROWTH OF RAT BONE MARROW CELLS IN VITRO

1968 ◽  
Vol 46 (5) ◽  
pp. 595-605 ◽  
Author(s):  
TR Bradley ◽  
R Siemienowicz
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Colony Growth ◽  
Rat Bone ◽  
Marrow Cells

scholarly journals Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin

2019 ◽  
Vol 20 (20) ◽  
pp. 4985 ◽  
Author(s):  
Hui-Lin Feng ◽  
Yen-Hua Chen ◽  
Sen-Shyong Jeng
Keyword(s):  
Bone Marrow ◽  
Recombinant Human Erythropoietin ◽  
Zinc Supplementation ◽  
Bone Marrow Cells ◽  
Human Erythropoietin ◽  
Post Surgery ◽  
Rat Bone ◽  
Marrow Cells

Anemia is a severe complication in patients with chronic kidney disease (CKD). Treatment with exogenous erythropoietin (EPO) can correct anemia in many with CKD. We produced 5/6-nephrectomized rats that became uremic and anemic at 25 days post surgery. Injection of the anemic 5/6-nephrectomized rats with 2.8 mg zinc/kg body weight raised their red blood cell (RBC) levels from approximately 85% of the control to 95% in one day and continued for 4 days. We compared the effect of ZnSO4 and recombinant human erythropoietin (rHuEPO) injections on relieving anemia in 5/6-nephrectomized rats. After three consecutive injections, both the ZnSO4 and rHuEPO groups had significantly higher RBC levels (98 ± 6% and 102 ± 6% of the control) than the saline group (90 ± 3% of the control). In vivo, zinc relieved anemia in 5/6-nephrectomized rats similar to rHuEPO. In vitro, we cultured rat bone marrow cells supplemented with ZnCl2, rHuEPO, or saline. In a 4-day suspension culture, we found that zinc induced erythropoiesis similar to rHuEPO. When rat bone marrow cells were supplement-cultured with zinc, we found that zinc stimulated the production of EPO in the culture medium and that the level of EPO produced was dependent on the concentration of zinc supplemented. The production of EPO via zinc supplementation was involved in the process of erythropoiesis.

Hepatocyte Growth Factor Induces Differentiation of Adult Rat Bone Marrow Cells into a Hepatocyte Lineage in Vitro

2000 ◽  
Vol 279 (2) ◽  
pp. 500-504 ◽  
Author(s):  
Seh-Hoon Oh ◽  
Masahiro Miyazaki ◽  
Hirosuke Kouchi ◽  
Yusuke Inoue ◽  
Masakiyo Sakaguchi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Bone Marrow Cells ◽  
Adult Rat ◽  
Hepatocyte Growth ◽  
Rat Bone ◽  
Marrow Cells

Effect of Normal and Neutropenic Dog Sera on Nucleic Acids of Rat Bone Marrow Cells in vitro*

1986 ◽  
Vol 33 (1-10) ◽  
pp. 123-130 ◽  
Author(s):  
G. A. Montes ◽  
A. S. Islas ◽  
E. Ocharan ◽  
J. A. Correa ◽  
W. G. Rudolph
Keyword(s):  
Bone Marrow ◽  
Nucleic Acids ◽  
Bone Marrow Cells ◽  
Rat Bone ◽  
Marrow Cells

scholarly journals ФЕНОТИПОВІ ТА МОРФОЛОГІЧНІ ЗМІНИ КУЛЬТУРИ КЛІТИН КІСТКОВОГО МОЗКУ ЩУРІВ В ПРОЦЕСІ ЇХ КУЛЬТИВУВАННЯ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 126-132
Author(s):  
A.I. Mazurkiewicz ◽  
V.V. Kovpak ◽  
O.S. Kovpak
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Morphological Changes ◽  
Embryonic Stem ◽  
In Vitro Cultivation ◽  
Rat Bone ◽  
Marrow Cells

Bone marrow is the only adult tissue which normally consists of immature undifferentiated and low differentiated cells which called stem cells and they are similar in structure to embryonic stem cells. But literature data analysis doesn't give an unambiguous answer regarding phenotypic and morphological changes of bone marrow cells culture of rats during their in vitro cultivation which necessitated further research.Investigate phenotypic and morphological changes of bone marrow cells culture of rats during their in vitro cultivation from first to fourth passage.We were used in these research bone marrow cells of rats from the first to the fourth passages. Microscopic analysis and evaluation morphological changes of bone marrow cells culture of rats during cultivation were carried out using inverted microscope Axiovert 40. Control of changes phenotype was performed by detecting CD markers (CD10, CD38, CD34, CD45, CD48, CD54, CD56, CD66e, CD96, CD227, CD326, pan–keratin). The evaluation was performed by the semi– quantitative method (H–Score).The research of primary culture of rat bone marrow cells showed that it morphologically heterogeneous, noted the small number of cells polygonal shape, surrounded by the fibroblast cells. During the cultivation cell culture becomes more homogenous at the expense of fibroblast–like cells. As a result of occurred the transition process from heterogeneous culture in zero passage to the most homogeneous culture in 4 passage. Immunophenotyping population of cell culture derived from rat bone marrow, revealed a high level of expression of pan–keratin; moderate level – CD34, CD48, CD66e, CD95; low level – CD38, CD45, CD56, CD227, CD326; lack of expression – CD10, CD54. Change of the expression of surface markers varies in each passage CD48, CD66e, CD95 increased significantly; CD38, SD45, SD326, pan–keratin reduced significantly. The markers CD34, CD 56, CD 227 were expressed on the one level from the first to the fourth passage. The expression of the CD10, CD54 markers during the study period was not identified.

In Vitro Osteogenic Response of Rat Bone Marrow Cells to bFGF and BMP-2 Treatments

2006 ◽  
Vol 443 (:) ◽  
pp. 113-123 ◽  
Author(s):  
Mathew Varkey ◽  
Cezary Kucharski ◽  
Takrima Haque ◽  
Walter Sebald ◽  
Hasan Uluda??
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Osteogenic Response ◽  
Rat Bone ◽  
Marrow Cells

scholarly journals Inhibition of human macrophage colony formation by interleukin 4.

1989 ◽  
Vol 170 (2) ◽  
pp. 577-582 ◽  
Author(s):  
J H Jansen ◽  
G J Wientjens ◽  
W E Fibbe ◽  
R Willemze ◽  
H C Kluin-Nelemans
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Bone Marrow Cells ◽  
Direct Action ◽  
Colony Growth ◽  
Interleukin 4 ◽  
Human Macrophage ◽  
Macrophage Colony ◽  
Marrow Cells

We investigated the effects of human rIL-4 on in vitro hematopoiesis. A profound inhibition of macrophage colony formation by IL-4 was observed, whereas colony growth of other lineages was not affected. Inhibition of macrophage colony growth was not restricted to GM-CSF-induced colony growth but was also present in cultures stimulated with M-CSF. This inhibition was not only observed in cultures of light density bone marrow cells, but also in cultures of monocyte- and T lymphocyte-depleted bone marrow cells. Since a similar inhibition was observed in cultures of CD34+HLA-DR+-enriched bone marrow cells, a direct action of IL-4 on monocyte-committed progenitor cells is suggested.

The in vitro formation of a calcified extracellular matrix on biomaterials by rat bone marrow cells

Bone ◽  
1992 ◽  
Vol 13 (1) ◽  
pp. 99-99
Author(s):  
J.D. de Bruijn ◽  
C.P.A.T. Klein ◽  
K. de Groot ◽  
J.E. Davies ◽  
C.A. van Blitterswijk
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Bone Marrow Cells ◽  
Rat Bone ◽  
Marrow Cells
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