STIMULATION OF MOUSE BONE MARROW COLONY GROWTH IN VITRO BY CONDITIONED MEDIUM

1968 ◽  
Vol 46 (5) ◽  
pp. 607-618 ◽  
Author(s):  
TR Bradley ◽  
Margaret A Sumner
Keyword(s):  
Bone Marrow ◽  
Conditioned Medium ◽  
Colony Growth ◽  
Mouse Bone Marrow ◽  
Stimulation Of

scholarly journals Stimulation of eosinophil production in vitro by eosinophilopoietin and spleen-cell-derived eosinophil growth-stimulating factor

Blood ◽  
1980 ◽  
Vol 56 (4) ◽  
pp. 706-711 ◽  
Author(s):  
SH Bartelmez ◽  
WH Dodge ◽  
AA Mahmoud ◽  
DA Bass
Keyword(s):  
Bone Marrow ◽  
Liquid Culture ◽  
Mouse Bone Marrow ◽  
Response Curves ◽  
Liquid Culture System ◽  
Similar Dose ◽  
Stimulating Factor ◽  
Stimulation Of

Abstract Eosinophilopoietin (EPP) was previously characterized by the ability to stimulate eosinophil production in vivo, but these studies could not ascertain whether EPP had a direct effect on the bone marrow or acted indirectly by causing release of eosinophilopoietic activity by other tissues. The present studies demonstrate that EPP stimulates eosinophil growth in liquid culture of mouse bone marrow in vitro. The timing of stimulation by EPP in vivo and in vitro were parallel, with maximal eosinophil growth after 48 hr. Moreover, EPP appears similar to, and possible identical with, the eosinophil growth-stimulating substance (EO-GSF) released by antigenic stimulation of immune nonadherent spleen cells. Both EPP and EO-GSF are of low molecular weight, both produce stimulation of eosinophil growth with identical kinetics, and both produced similar dose-response curves in the liquid culture system.

scholarly journals Cell interactions influencing murine marrow megakaryocytes: nature of the potentiator cell in bone marrow

Blood ◽  
1981 ◽  
Vol 57 (1) ◽  
pp. 157-163 ◽  
Author(s):  
N Williams ◽  
H Jackson ◽  
P Ralph ◽  
I Nakoinz
Keyword(s):  
Bone Marrow ◽  
Conditioned Medium ◽  
Colony Formation ◽  
Bone Marrow Cells ◽  
Buoyant Density ◽  
Colony Growth ◽  
Mouse Bone Marrow ◽  
Bone Marrow Cultures ◽  
Cell Conditioned Medium ◽  
Marrow Cells

Abstract Auxiliary bone marrow cells are required for optimal murine megakaryocyte colony formation in addition to progenitor cells and a colony stimulating activity (CSA) present in WEHI-3 cell conditioned medium. These auxiliary cells are adherent, with a sedimentation rate of 5.8 mm hr-1 and buoyant density of 1.065–1.078 gcm-3. The activity from bone marrow cells is loss at irradiation doses above 900 rad. Bone marrow cells with these characteristics, and supernatants from lung, bone shafts, and peritoneal exudate cells were all active in enhancing megakaryocyte colony incidences in mouse bone marrow cultures above those stimulated by an obligatory activity in WEHI-3 cell conditioned medium. Certain macrophage cell lines (J774, P388D1) could elaborate the activity. This study confirms that a potentiation activity enhances CSA stimulation of megakaryocyte colony formation. The potentiator is elaborated by bone marrow cells in limiting amounts requiring either high cell concentrations or an exogenous source of the activity for optimal colony growth.

scholarly journals Brief Report: Stimulation of Bone Marrow Colony Growth In Vitro by Human Urine

Blood ◽  
1969 ◽  
Vol 33 (3) ◽  
pp. 396-399 ◽  
Author(s):  
W. A. ROBINSON ◽  
E. R. STANLEY ◽  
D. METCALF
Keyword(s):  
Bone Marrow ◽  
Cell Growth ◽  
Mononuclear Cell ◽  
Human Urine ◽  
Colony Growth ◽  
Urine Samples ◽  
Disease States ◽  
Normal Humans ◽  
Stimulation Of

Abstract Using a new technic of bone marrow culture in agar, urine samples from 50 humans have been tested for their ability to stimulate the formation of granulocyte—mononuclear cell growth in vitro. Significant colony stimulating activity has been found with 25 out of 50 unconcentrated urine samples from both normal humans and patients with a variety of disease states.

scholarly journals Stimulation of DNA Synthesis In Vitro in Mouse Thymic Cells by Bone Marrow Preparation

Blood ◽  
1971 ◽  
Vol 37 (2) ◽  
pp. 231-239 ◽  
Author(s):  
AHUVA KNYSZYNSKI ◽  
M. BURGER
Keyword(s):  
Bone Marrow ◽  
Protein Synthesis ◽  
Dna Synthesis ◽  
Thymidine Incorporation ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Total Body ◽  
Very High ◽  
Stimulation Of

Abstract Some characteristics of a bone marrow factor were ascertained, on the basis of its ability to increase 3H-thymidine incorporation into thymic cells in vitro. It was found that the factor was not detectable in kidney or spleen preparations derived from the same mice from which active bone marrow preparations were obtained. The activity of the factor was reduced shortly after total-body irradiation of the mice, and increased again during the regeneration of the bone marrow. It is further shown here that the bone marrow factor has no effect on 3H-thymidine incorporation into mouse bone marrow cells. Leukemic mouse thymic cells show a significantly reduced response towards the bone marrow factor in comparison to normal thymic cells. The bone marrow factor has less effect on protein synthesis than on DNA synthesis in thymic cells, and its stimulatory effect on DNA synthesis is not dependent on protein synthesis. The isolated bone marrow factor is resistant towards heating at 100°C, even at a very low or very high pH. However, it is more labile to heat when in an impure state. Its activity is not affected by RNase, DNase, pronase and carboxypeptidase. It is insoluble in ether, but soluble in absolute ethanol.

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