scholarly journals Effects of acute versus post-acute systemic delivery of neural progenitor cells on neurological recovery and brain remodeling after focal cerebral ischemia in mice

2014 ◽  
Vol 5 (8) ◽  
pp. e1386-e1386 ◽  
Author(s):  
T R Doeppner ◽  
B Kaltwasser ◽  
M K Teli ◽  
E Bretschneider ◽  
M Bähr ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Focal Cerebral Ischemia ◽  
Neural Progenitor ◽  
Neurological Recovery ◽  
Systemic Delivery

Abstract 133: Transplantation Of Neurosphere-forming Stem Cells Into Circulating Blood Attenuates Acute Inflammatory Activation Of Both Brain And Spleen After Cerebrovascular Ischemic Injury.

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Soon-Tae Lee ◽  
Kon Chu ◽  
Keun-Hwa Jung ◽  
Hee-Kwon Park ◽  
Jeong-Min Kim ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Progenitor Cells ◽  
Inflammatory Cytokines ◽  
Neural Progenitor Cells ◽  
Focal Cerebral Ischemia ◽  
Neural Progenitor ◽  
Control Group ◽  
Tissue Ischemia ◽  
Realtime Pcr ◽  
Inflammatory Activation

Background and Objectives: Transplantation of neural progenitor cells (NPCs) has been studied to achieve the regeneration of damaged brain in experimental stroke models. Tissue ischemia induces systemic inflammatory activation, especially in spleen. However, the interaction between NPCs and immune/inflammatory cells has not been studied in ischemia models. In this study, we investigated whether NPC can attenuate cerebral and systemic inflammatory activation in focal cerebral ischemia. Methods: Focal cerebral ischemia was induced by intraluminal thread occlusion of the middle cerebral artery for 60 minutes (c57/bl6 adult male mice), and mouse NPC from primary neurosphere (1 million cells) or vehicle (PBS, control group) was injected via tail veins with cerebral reperfusion. Inflammatory cytokines were evaluated with RealTime-PCR at 22 hrs, and the weights of each spleen and thymus were measured. Results: Cerebral ischemia induced progressive atrophies of spleen and thymus when measured at 22 hr and 96 hr after ischemia. Intravenous injection of NPC accelerated these lymphoid organ atrophies, and the weights of spleen and thymus were significantly lower in NPC-injected mice at 96 hr after the ischemia compared to the vehicle injected mice. RealTime-PCR analysis using ischemic hemispheres and spleens showed reduced expressions of inflammatory cytokines, including TNF-alpha, IL-6, MIP-2, IL-13, FoxP3, IL-1beta, and IP10 in NPC-injected brains and spleens at 22 hr after the ischemia. Transplanted NPCs were detected in systemic organs, and mostly in spleen. NPC-injected mice showed reduced infarct volume and neurologic deficits. Conclusion: Intravenous transplantation of primary neuro-sphere cells in cerebral ischemia attenuated systemic inflammatory activation, which suggests that the immunomodulating property of neural progenitor cells can be applicable for preventing harmful inflammations after the tissue ischemia.

Injection of neural progenitor cells improved learning and memory dysfunction after cerebral ischemia

2008 ◽  
Vol 211 (1) ◽  
pp. 194-202 ◽  
Author(s):  
Nobuyuki Mochizuki ◽  
Norio Takagi ◽  
Koji Kurokawa ◽  
Chika Onozato ◽  
Yoshiyuki Moriyama ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Progenitor Cells ◽  
Learning And Memory ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Memory Dysfunction ◽  
Improved Learning

Conditioned Medium Derived from Neural Progenitor Cells Induces Long-term Post-ischemic Neuroprotection, Sustained Neurological Recovery, Neurogenesis, and Angiogenesis

2016 ◽  
Vol 54 (2) ◽  
pp. 1531-1540 ◽  
Author(s):  
Thorsten R. Doeppner ◽  
Viktorija Traut ◽  
Alexander Heidenreich ◽  
Britta Kaltwasser ◽  
Bert Bosche ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Conditioned Medium ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Neurological Recovery

scholarly journals Effects of Administration Route on Migration and Distribution of Neural Progenitor Cells Transplanted into Rats with Focal Cerebral Ischemia, an MRI Study

2009 ◽  
Vol 30 (3) ◽  
pp. 653-662 ◽  
Author(s):  
Lian Li ◽  
Quan Jiang ◽  
Guangliang Ding ◽  
Li Zhang ◽  
Zheng Gang Zhang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Focal Cerebral Ischemia ◽  
Three Dimensional ◽  
Neural Progenitor ◽  
Artery Occlusion ◽  
Blue Staining ◽  
Ischemic Lesion ◽  
Administration Routes ◽  
Male Wistar Rats

We tested the hypotheses that administration routes affect the migration and distribution of grafted neural progenitor cells (NPCs) in the ischemic brain and that the ischemic lesion plays a role in mediating the grafting process. Male Wistar rats ( n=41) were subjected to 2-h middle cerebral artery occlusion (MCAo), followed 1 day later by administration of magnetically labeled NPCs. Rats with MCAo were assigned to one of three treatment groups targeted for cell transplantation intra-arterially (IA), intracisternally (IC), or intravenously (IV). MRI measurements consisting of T2-weighted imaging and three-dimensional (3D) gradient echo imaging were performed 24 h after MCAo, 4 h after cell injection, and once a day for 4 days. Prussian blue staining was used to identify the labeled cells, 3D MRI to detect cell migration and distribution, and T2 map to assess lesion volumes. Intra-arterial (IA) administration showed significantly increased migration, a far more diffuse distribution pattern, and a larger number of transplanted NPCs in the target brain than IC or IV administration. However, high mortality with IA delivery (IA: 41%; IC: 17%; IV: 8%) poses a serious concern for using this route of administration. Animals with smaller lesions at the time of transplantation have fewer grafted cells in the parenchyma.

scholarly journals Intravenous injection of neural progenitor cells facilitates angiogenesis after cerebral ischemia

2012 ◽  
Vol 3 (2) ◽  
pp. 43-53 ◽  
Author(s):  
Yoshiyuki Moriyama ◽  
Norio Takagi ◽  
Kanae Hashimura ◽  
Chisa Itokawa ◽  
Kouichi Tanonaka
Keyword(s):  
Cerebral Ischemia ◽  
Progenitor Cells ◽  
Intravenous Injection ◽  
Neural Progenitor Cells ◽  
Neural Progenitor

Delayed injection of neural progenitor cells improved spatial learning dysfunction after cerebral ischemia

2008 ◽  
Vol 368 (1) ◽  
pp. 151-156 ◽  
Author(s):  
Nobuyuki Mochizuki ◽  
Norio Takagi ◽  
Chika Onozato ◽  
Yoshiyuki Moriyama ◽  
Satoshi Takeo ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Progenitor Cells ◽  
Spatial Learning ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Learning Dysfunction
Sign in / Sign up

Export Citation Format

Share Document