scholarly journals BRD4 regulates Nanog expression in mouse embryonic stem cells and preimplantation embryos

2014 ◽  
Vol 21 (12) ◽  
pp. 1950-1960 ◽  
Author(s):  
W Liu ◽  
P Stein ◽  
X Cheng ◽  
W Yang ◽  
N-Y Shao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Preimplantation Embryos ◽  
Nanog Expression

scholarly journals Correction: Perfluorooctane Sulfonate Disturbs Nanog Expression through miR-490-3p in Mouse Embryonic Stem Cells

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0221486
Author(s):  
Bo Xu ◽  
Xiaojiao Chen ◽  
Zhilei Mao ◽  
Minjian Chen ◽  
Xiumei Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Perfluorooctane Sulfonate ◽  
Mouse Embryonic Stem Cells ◽  
Nanog Expression

scholarly journals Inhibition of β-catenin–TCF1 interaction delays differentiation of mouse embryonic stem cells

2015 ◽  
Vol 211 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Sujash S. Chatterjee ◽  
Abil Saj ◽  
Tenzin Gocha ◽  
Matthew Murphy ◽  
Foster C. Gonsalves ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Fate ◽  
Protein Interactions ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Protein Protein Interactions ◽  
Nanog Expression ◽  
Context Specific

The ability of mouse embryonic stem cells (mESCs) to self-renew or differentiate into various cell lineages is regulated by signaling pathways and a core pluripotency transcriptional network (PTN) comprising Nanog, Oct4, and Sox2. The Wnt/β-catenin pathway promotes pluripotency by alleviating T cell factor TCF3-mediated repression of the PTN. However, it has remained unclear how β-catenin’s function as a transcriptional activator with TCF1 influences mESC fate. Here, we show that TCF1-mediated transcription is up-regulated in differentiating mESCs and that chemical inhibition of β-catenin/TCF1 interaction improves long-term self-renewal and enhances functional pluripotency. Genetic loss of TCF1 inhibited differentiation by delaying exit from pluripotency and conferred a transcriptional profile strikingly reminiscent of self-renewing mESCs with high Nanog expression. Together, our data suggest that β-catenin’s function in regulating mESCs is highly context specific and that its interaction with TCF1 promotes differentiation, further highlighting the need for understanding how its individual protein–protein interactions drive stem cell fate.

scholarly journals YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1123
Author(s):  
Raquel Pérez-Palacios ◽  
María Climent ◽  
Javier Santiago-Arcos ◽  
Sofía Macías-Redondo ◽  
Martin Klar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Preimplantation Development ◽  
Preimplantation Embryos ◽  
Yin Yang

Yin Yang 2 encodes a mammalian-specific transcription factor (YY2) that shares high homology in the zinc finger region with both YY1 and REX1/ZFP42, encoded by the Yin Yang 1 and Reduced Expression Protein 1/Zinc Finger Protein 42 gene, respectively. In contrast to the well-established roles of the latter two in gene regulation, X chromosome inactivation and binding to specific transposable elements (TEs), much less is known about YY2, and its presence during mouse preimplantation development has not been described. As it has been reported that mouse embryonic stem cells (mESC) cannot be propagated in the absence of Yy2, the mechanistic understanding of how Yy2 contributes to mESC maintenance remains only very partially characterized. We describe Yy2 expression studies using RT-PCR and staining with a high-affinity polyclonal serum in mouse embryos and mESC. Although YY2 is expressed during preimplantation development, its presence appears dispensable for developmental progress in vitro until formation of the blastocyst. Attenuation of Yy2 levels failed to alter either Zscan4 levels in two-cell embryos or IAP and MERVL levels at later preimplantation stages. In contrast to previous claims that constitutively expressed shRNA against Yy2 in mESC prohibited the propagation of mESC in culture, we obtained colonies generated from mESC with attenuated Yy2 levels. Concomitant with a decreased number of undifferentiated colonies, Yy2-depleted mESC expressed higher levels of Zscan4 but no differences in the expression of TEs or other pluripotency markers including Sox2, Oct4, Nanog and Esrrb were observed. These results confirm the contribution of Yy2 to the maintenance of mouse embryonic stem cells and show the preimplantation expression of YY2. These functions are discussed in relation to mammalian-specific functions of YY1 and REX1.

scholarly journals SIRT1 Regulates Apoptosis and Nanog Expression in Mouse Embryonic Stem Cells by Controlling p53 Subcellular Localization

2008 ◽  
Vol 2 (3) ◽  
pp. 241-251 ◽  
Author(s):  
Myung-Kwan Han ◽  
Eun-Kyung Song ◽  
Ying Guo ◽  
Xuan Ou ◽  
Charlie Mantel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Subcellular Localization ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Nanog Expression

scholarly journals E-cadherin and, in Its Absence, N-cadherin Promotes Nanog Expression in Mouse Embryonic Stem Cells via STAT3 Phosphorylation

Stem Cells ◽  
2012 ◽  
Vol 30 (9) ◽  
pp. 1842-1851 ◽  
Author(s):  
Kate Hawkins ◽  
Lisa Mohamet ◽  
Sarah Ritson ◽  
Catherine L. R. Merry ◽  
Christopher M. Ward
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Nanog Expression ◽  
Stat3 Phosphorylation ◽  
E Cadherin

scholarly journals Perfluorooctane Sulfonate Disturbs Nanog Expression through miR-490-3p in Mouse Embryonic Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e74968 ◽  
Author(s):  
Bo Xu ◽  
Xiaojiao Chen ◽  
Zhilei Mao ◽  
Minjian Chen ◽  
Xiumei Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Perfluorooctane Sulfonate ◽  
Mouse Embryonic Stem Cells ◽  
Nanog Expression
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