Mouse embryonic stem cells and preimplantation embryos require signaling through the phosphatidylinositol 3-kinase pathway to suppress apoptosis

2004 ◽  
Vol 70 (3) ◽  
pp. 324-332 ◽  
Author(s):  
Vera S. Gross ◽  
Mailee Hess ◽  
Geoffrey M. Cooper
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Preimplantation Embryos ◽  
Phosphatidylinositol 3 Kinase ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3

scholarly journals BRD4 regulates Nanog expression in mouse embryonic stem cells and preimplantation embryos

2014 ◽  
Vol 21 (12) ◽  
pp. 1950-1960 ◽  
Author(s):  
W Liu ◽  
P Stein ◽  
X Cheng ◽  
W Yang ◽  
N-Y Shao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Preimplantation Embryos ◽  
Nanog Expression

scholarly journals YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1123
Author(s):  
Raquel Pérez-Palacios ◽  
María Climent ◽  
Javier Santiago-Arcos ◽  
Sofía Macías-Redondo ◽  
Martin Klar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Preimplantation Development ◽  
Preimplantation Embryos ◽  
Yin Yang

Yin Yang 2 encodes a mammalian-specific transcription factor (YY2) that shares high homology in the zinc finger region with both YY1 and REX1/ZFP42, encoded by the Yin Yang 1 and Reduced Expression Protein 1/Zinc Finger Protein 42 gene, respectively. In contrast to the well-established roles of the latter two in gene regulation, X chromosome inactivation and binding to specific transposable elements (TEs), much less is known about YY2, and its presence during mouse preimplantation development has not been described. As it has been reported that mouse embryonic stem cells (mESC) cannot be propagated in the absence of Yy2, the mechanistic understanding of how Yy2 contributes to mESC maintenance remains only very partially characterized. We describe Yy2 expression studies using RT-PCR and staining with a high-affinity polyclonal serum in mouse embryos and mESC. Although YY2 is expressed during preimplantation development, its presence appears dispensable for developmental progress in vitro until formation of the blastocyst. Attenuation of Yy2 levels failed to alter either Zscan4 levels in two-cell embryos or IAP and MERVL levels at later preimplantation stages. In contrast to previous claims that constitutively expressed shRNA against Yy2 in mESC prohibited the propagation of mESC in culture, we obtained colonies generated from mESC with attenuated Yy2 levels. Concomitant with a decreased number of undifferentiated colonies, Yy2-depleted mESC expressed higher levels of Zscan4 but no differences in the expression of TEs or other pluripotency markers including Sox2, Oct4, Nanog and Esrrb were observed. These results confirm the contribution of Yy2 to the maintenance of mouse embryonic stem cells and show the preimplantation expression of YY2. These functions are discussed in relation to mammalian-specific functions of YY1 and REX1.

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