scholarly journals Carbocisteine attenuates TNF-α-induced inflammation in human alveolar epithelial cells in vitro through suppressing NF-κB and ERK1/2 MAPK signaling pathways

2016 ◽  
Vol 37 (5) ◽  
pp. 629-636 ◽  
Author(s):  
Wei Wang ◽  
Wei-jie Guan ◽  
Rong-quan Huang ◽  
Yan-qing Xie ◽  
Jin-ping Zheng ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Signaling Pathways ◽  
Mapk Signaling ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Tnf Α ◽  
Mapk Signaling Pathways ◽  
Human Alveolar Epithelial Cells

scholarly journals Nuclear Factor-kappaB Regulates the Transcription of NADPH Oxidase 1 in Human Alveolar Epithelial Cells

2020 ◽  
Author(s):  
Weijing Wu ◽  
Li Li ◽  
Xiaoshan Su ◽  
ZHIXING ZHU ◽  
Xiaoping Lin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
A549 Cells ◽  
Alveolar Epithelial Cells ◽  
Ros Generation ◽  
Alveolar Epithelial ◽  
Tnf Α ◽  
Potential Regulatory Role ◽  
Human Alveolar Epithelial Cells

Abstract Objective Acute lung injury (ALI) is characterized by inflammation and oxidative stress. Nuclear factor-kappaB (NF-κB) mediates the expression of various inflammation-related genes, including the NADPH oxidase family. This study aimed to identify the potential regulatory role of NF-𝜅B on NADPH oxidases in TNF-α-induced oxidative stress in human alveolar epithelial cells. Methods Type II alveolar epithelial cell-derived A549 cells were treated with TNF-α for 24 hours to establish ALI cell models. RT-PCR, western blot, DCFH-DA ROS assay, Alibaba 2.1 online analysis, electrophoretic mobility shift assays and luciferase reporter analysis were employed to identify the potential regulatory role of NF-𝜅B on NADPH oxidases in TNF-α-induced oxidative stress in human alveolar epithelial cells. Results The expression of NF-κB/p65 was notably upregulated in TNF-α-stimulated A549 cells. NF-κB knockdown by siRNA significantly inhibited the TNF-α-induced ROS generation. Moreover, NF-𝜅B/p65 siRNA could inhibite the activation of NOX1, NOX2 and NOX4 mRNA and protein expression in TNF-α-stimulated A549 cells. The next study demonstrated that NF-𝜅B activated the transcription of NOX1 by binding to the -261 to -252 bp (NOX1/κB2, TAAAAATCCC) region of NOX1 promoter in TNF-α-stimulated A549 cells. Conclusion Our data demonstrated that NF-κB can aggravate TNF-α-induced ALI by regulating the activation of ROS generation and the expression of NOX1, NOX2 and NOX4. Moreover, NF-𝜅B could promote the NOX1 transcriptional activity via binding its promoter in TNF-α-stimulated A549 cells.

Microrna 155-3P modulates the FASL-mediated inflammation in human alveolar epithelial cells in vitro

2019 ◽  
Vol 493 ◽  
pp. S573-S574
Author(s):  
G. Sánchez ◽  
R. Herrero ◽  
A. Ferruelo ◽  
R. Murillo ◽  
Y. Douhal ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells

Zinc-dependent cytoadherence of Legionella pneumophila to human alveolar epithelial cells in vitro

2007 ◽  
Vol 43 (5-6) ◽  
pp. 234-242 ◽  
Author(s):  
Diaraf Farba Yaradou ◽  
Dominique Raze ◽  
Christophe Ginevra ◽  
Florence Ader ◽  
Anne Doléans-Jordheim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells
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