Expression of IL-6 and TNF-α in human alveolar epithelial cells is induced by invading, but not by adhering, Legionella pneumophila

2004 ◽  
Vol 37 (6) ◽  
pp. 295-302 ◽  
Author(s):  
Bin Chang ◽  
Junko Amemura-Maekawa ◽  
Fumiaki Kura ◽  
Ikuo Kawamura ◽  
Haruo Watanabe
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Tnf Α ◽  
Human Alveolar Epithelial Cells

scholarly journals Nuclear Factor-kappaB Regulates the Transcription of NADPH Oxidase 1 in Human Alveolar Epithelial Cells

2020 ◽  
Author(s):  
Weijing Wu ◽  
Li Li ◽  
Xiaoshan Su ◽  
ZHIXING ZHU ◽  
Xiaoping Lin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
A549 Cells ◽  
Alveolar Epithelial Cells ◽  
Ros Generation ◽  
Alveolar Epithelial ◽  
Tnf Α ◽  
Potential Regulatory Role ◽  
Human Alveolar Epithelial Cells

Abstract Objective Acute lung injury (ALI) is characterized by inflammation and oxidative stress. Nuclear factor-kappaB (NF-κB) mediates the expression of various inflammation-related genes, including the NADPH oxidase family. This study aimed to identify the potential regulatory role of NF-𝜅B on NADPH oxidases in TNF-α-induced oxidative stress in human alveolar epithelial cells. Methods Type II alveolar epithelial cell-derived A549 cells were treated with TNF-α for 24 hours to establish ALI cell models. RT-PCR, western blot, DCFH-DA ROS assay, Alibaba 2.1 online analysis, electrophoretic mobility shift assays and luciferase reporter analysis were employed to identify the potential regulatory role of NF-𝜅B on NADPH oxidases in TNF-α-induced oxidative stress in human alveolar epithelial cells. Results The expression of NF-κB/p65 was notably upregulated in TNF-α-stimulated A549 cells. NF-κB knockdown by siRNA significantly inhibited the TNF-α-induced ROS generation. Moreover, NF-𝜅B/p65 siRNA could inhibite the activation of NOX1, NOX2 and NOX4 mRNA and protein expression in TNF-α-stimulated A549 cells. The next study demonstrated that NF-𝜅B activated the transcription of NOX1 by binding to the -261 to -252 bp (NOX1/κB2, TAAAAATCCC) region of NOX1 promoter in TNF-α-stimulated A549 cells. Conclusion Our data demonstrated that NF-κB can aggravate TNF-α-induced ALI by regulating the activation of ROS generation and the expression of NOX1, NOX2 and NOX4. Moreover, NF-𝜅B could promote the NOX1 transcriptional activity via binding its promoter in TNF-α-stimulated A549 cells.

Zinc-dependent cytoadherence of Legionella pneumophila to human alveolar epithelial cells in vitro

2007 ◽  
Vol 43 (5-6) ◽  
pp. 234-242 ◽  
Author(s):  
Diaraf Farba Yaradou ◽  
Dominique Raze ◽  
Christophe Ginevra ◽  
Florence Ader ◽  
Anne Doléans-Jordheim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells

scholarly journals Nuclear factor-kappaB regulates the transcription of NADPH oxidase 1 in human alveolar epithelial cells

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Weijing Wu ◽  
Li Li ◽  
Xiaoshan Su ◽  
Zhixing Zhu ◽  
Xiaoping Lin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
A549 Cells ◽  
Alveolar Epithelial Cells ◽  
Nuclear Factor ◽  
Alveolar Epithelial ◽  
Tnf Α ◽  
Potential Regulatory Role ◽  
Human Alveolar Epithelial Cells

Abstract Objective Acute lung injury (ALI) is characterized by inflammation and oxidative stress. Nuclear factor-kappaB (NF-κB) mediates the expression of various inflammation-related genes, including the NADPH oxidase family. This study aimed to identify the potential regulatory role of NF-κB on NADPH oxidases in tumor necrosis factor-α (TNF-α)-induced oxidative stress in human alveolar epithelial cells. Methods A549 cells were treated with TNF-α for 24 h to establish ALI cell models. RT-PCR, western blot, assessment of oxidative stress, Alibaba 2.1 online analysis, electrophoretic mobility shift assays and luciferase reporter analysis were employed to identify the potential regulatory role of NF-κB on NADPH oxidases in TNF-α-induced oxidative stress in human alveolar epithelial cells. Results The expression of NF-κB/p65 was notably upregulated in TNF-α-stimulated A549 cells. NF-κB knockdown by siRNA significantly inhibited the TNF-α-induced oxidative stress. Moreover, NF-κB/p65 siRNA could inhibit the activation of NOX1, NOX2 and NOX4 mRNA and protein expression in TNF-α-stimulated A549 cells. The next study demonstrated that NF-κB activated the transcription of NOX1 by binding to the -261 to -252 bp (NOX1/κB2, TAAAAATCCC) region of NOX1 promoter in TNF-α-stimulated A549 cells. Conclusion Our data demonstrated that NF-κB can aggravate TNF-α-induced ALI by regulating the oxidative stress response and the expression of NOX1, NOX2 and NOX4. Moreover, NF-κB could promote the NOX1 transcriptional activity via binding its promoter in TNF-α-stimulated A549 cells.

Infectivity of Legionella pneumophila mip mutant for alveolar epithelial cells

1995 ◽  
Vol 30 (4) ◽  
pp. 247-250 ◽  
Author(s):  
Nicholas P. Cianciotto ◽  
Julie Kim Stamos ◽  
David W. Kamp
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial

Resolvin E1 Improves Mitochondrial Function in Human Alveolar Epithelial Cells during Severe Inflammation

Lipids ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 53-65 ◽  
Author(s):  
Konstantin Mayer ◽  
Natascha Sommer ◽  
Karl Hache ◽  
Andreas Hecker ◽  
Sylvia Reiche ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mitochondrial Function ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Severe Inflammation ◽  
Human Alveolar Epithelial Cells

Cellular Toxicity of Various Inhalable Metal Nanoparticles on Human Alveolar Epithelial Cells

2007 ◽  
Vol 19 (sup1) ◽  
pp. 59-65 ◽  
Author(s):  
Seoyoung Park ◽  
Yong Kwon Lee ◽  
Moonju Jung ◽  
Ki Heon Kim ◽  
Namhyun Chung ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Metal Nanoparticles ◽  
Alveolar Epithelial Cells ◽  
Cellular Toxicity ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells

Ox-LDL-induced TGF-β1 production in human alveolar epithelial cells: Involvement of the Ras/ERK/PLTP pathway

2012 ◽  
Vol 227 (9) ◽  
pp. 3185-3191 ◽  
Author(s):  
Ling-Li Guo ◽  
Ya-Juan Chen ◽  
Tao Wang ◽  
Jing An ◽  
Cheng-Na Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Tgf Β1 ◽  
Human Alveolar Epithelial Cells

scholarly journals CCR2 and CXCR3 agonistic chemokines are differently expressed and regulated in human alveolar epithelial cells type II

2005 ◽  
Vol 6 (1) ◽  
Author(s):  
Dmitri V Pechkovsky ◽  
Torsten Goldmann ◽  
Corinna Ludwig ◽  
Antje Prasse ◽  
Ekkehard Vollmer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Type Ii ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells
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