Zinc-dependent cytoadherence of Legionella pneumophila to human alveolar epithelial cells in vitro

2007 ◽  
Vol 43 (5-6) ◽  
pp. 234-242 ◽  
Author(s):  
Diaraf Farba Yaradou ◽  
Dominique Raze ◽  
Christophe Ginevra ◽  
Florence Ader ◽  
Anne Doléans-Jordheim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells

scholarly journals In VitroIntracellular Activity andIn VivoEfficacy of Modithromycin, a Novel Bicyclolide, againstLegionella pneumophila

2011 ◽  
Vol 55 (4) ◽  
pp. 1594-1597 ◽  
Author(s):  
Takafumi Sato ◽  
Kazuhiro Tateda ◽  
Soichiro Kimura ◽  
Yoshikazu Ishii ◽  
Keizo Yamaguchi
Keyword(s):  
Legionella Pneumophila ◽  
Alveolar Epithelial Cells ◽  
Intracellular Bacteria ◽  
Pulmonary Infections ◽  
Once Daily ◽  
Alveolar Epithelial ◽  
Bactericidal Activities ◽  
Human Alveolar Epithelial Cells

ABSTRACTThein vitroandin vivoactivities of modithromycin, a novel bicyclolide, againstLegionella pneumophilawere compared with those of telithromycin, clarithromycin, azithromycin, and levofloxacin. All the test agents decreased the intracellular growth of viableL. pneumophilabacteria over 96 h of incubation in both types of cells used, A/J mouse-derived macrophages and A549 human alveolar epithelial cells, at extracellular concentrations of 4× and 16× MIC, respectively. However, when the agents were removed from the medium after exposure for 2 h, regrowth of intracellular bacteria occurred in both cell systems when they were exposed to telithromycin, clarithromycin, and levofloxacin but not when they were exposed to modithromycin and azithromycin. Once-daily administration of modithromycin at a dose of 10 mg/kg of body weight for 5 days led to a significant decrease of intrapulmonary viableL. pneumophilabacteria in immunosuppressed A/J mice. The efficacy of modithromycin was superior to the efficacies of telithromycin and clarithromycin and comparable to the efficacies of azithromycin and levofloxacin. In addition, modithromycin and azithromycin inhibited the intrapulmonary regrowth of bacteria even at 72 h after the last treatment, but telithromycin and levofloxacin did not. These results suggested that modithromycin has longer-lasting cellular pharmacokinetic features like azithromycin. In conclusion, modithromycin, as well as azithromycin, has excellentin vitroandin vivobactericidal activities and persistent efficacy against intracellularL. pneumophila. Modithromycin should be a useful agent for treatment of pulmonary infections caused by this pathogen.

Microrna 155-3P modulates the FASL-mediated inflammation in human alveolar epithelial cells in vitro

2019 ◽  
Vol 493 ◽  
pp. S573-S574
Author(s):  
G. Sánchez ◽  
R. Herrero ◽  
A. Ferruelo ◽  
R. Murillo ◽  
Y. Douhal ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells
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