Hydrolysis of protein in vacuoles isolated from higher plant tissue

Nature ◽  
1979 ◽  
Vol 277 (5695) ◽  
pp. 412-413 ◽  
Author(s):  
MIKIO NISHIMURA ◽  
HARRY BEEVERS
Keyword(s):  
Plant Tissue ◽  
Higher Plant ◽  
Hydrolysis Of

On the role of higher plant and microbial lipases in the ruminal hydrolysis of grass lipids

1977 ◽  
Vol 38 (2) ◽  
pp. 225-232 ◽  
Author(s):  
R. M. C. Dawson ◽  
N. Hemington ◽  
G. P. Hazlewood
Keyword(s):  
Rumen Fluid ◽  
Higher Plant ◽  
Microbial Enzymes ◽  
Heat Treated ◽  
Ruminal Degradation ◽  
Complex Lipids ◽  
Microbial Lipases ◽  
Micro Organisms ◽  
Hydrolysis Of

1. The galactolipids of heat-treated, 14C-labelled rye grass S24 administered intraruminally to a sheep fed on an autoclaved diet were rapidly catabolized.2. When grass was homogenized with rumen contents devoid of higher plant lipases the grass galactolipids were rapidly metabolized, but were not metabolized when the rumen contents were boiled to destroy microbial galactolipases.3. 14C-labelled monogalactosyldiglyceride, digalactosyldiglyceride and triolein were metabolized, with the release of 14C-labelled fatty acids when incubated with a homogenate (100 g/l) of grass or clover in rumen fluid from a starved sheep, but not when the rumen fluid was heat-treated to destroy microbial enzymes.4. It is concluded that in the sheep the lipases of rumen micro-organisms play a major part in the ruminal degradation of ingested complex lipids of pasture.

Enzyme synthesis in higher plant tissue

Planta ◽  
1968 ◽  
Vol 84 (1) ◽  
pp. 94-96 ◽  
Author(s):  
J. Edelman ◽  
M. J. Bradshaw
Keyword(s):  
Plant Tissue ◽  
Enzyme Synthesis ◽  
Higher Plant

Phosphorus-31 nuclear magnetic resonance studies on higher plant tissue

Plant Science ◽  
1985 ◽  
Vol 38 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Fumiyuki Mitsumori ◽  
Tadakatsu Yoneyama ◽  
Osamu Ito
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Plant Tissue ◽  
Higher Plant ◽  
Magnetic Resonance Studies ◽  
Nuclear Magnetic

Enzyme Formation in Higher Plant Tissue:1

1969 ◽  
Vol 20 (1) ◽  
pp. 84-86 ◽  
Author(s):  
M. J. BRADSHAW ◽  
J. M. CHAPMAN ◽  
J. EDELMAN ◽  
P. L. MOORE
Keyword(s):  
Plant Tissue ◽  
Higher Plant ◽  
Enzyme Formation

Improved Extraction of Abscisic Acid From Plant Tissue

1988 ◽  
Vol 15 (3) ◽  
pp. 421 ◽  
Author(s):  
BR Loveys ◽  
HM van Dijk
Keyword(s):  
Abscisic Acid ◽  
Plant Tissue ◽  
Room Temperature ◽  
Internal Standard ◽  
Gas Chromatography Mass Spectrometry ◽  
Plant Tissues ◽  
Enzymic Hydrolysis ◽  
Solvent Residues ◽  
Hydrolysis Of ◽  
Extracting Solvent

Methods have been developed for the extraction of abscisic acid (ABA) from plant tissues using water as the extracting solvent. Abnormally high levels of ABA were extracted with water at room temperature from some tissues. This was attributed to enzymic hydrolysis of conjugated forms of ABA. This problem was overcome by briefly boiling the plant tissue in water before homogenisation. Subsequent analysis of ABA was by combined gas chromatography/mass spectrometry using deuterium-labelled ABA as an internal standard but it is suggested that the method may be particularly suited to assay procedures based on immunological techniques where solvent residues may be undesirable.

The estimation of phenylalanine ammonia-lyase (PAL)-activity in intact cells of higher plant tissue

Planta ◽  
1976 ◽  
Vol 131 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Nikolaus Amrhein ◽  
Karl-Heinz G�deke ◽  
J�rgen Gerhardt
Keyword(s):  
Plant Tissue ◽  
Phenylalanine Ammonia Lyase ◽  
Higher Plant ◽  
Intact Cells ◽  
Pal Activity
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