Differential Staining Patterns of Human Chromosomes treated with Potassium Permanganate

Nature ◽  
1972 ◽  
Vol 239 (5368) ◽  
pp. 168-170 ◽  
Author(s):  
T. UTAKOJI
Keyword(s):  
Potassium Permanganate ◽  
Differential Staining ◽  
Human Chromosomes

scholarly journals Differential Staining of Human Chromosomes by Treatment with Urea

1971 ◽  
Vol 47 (9) ◽  
pp. 729-731 ◽  
Author(s):  
Yukimasa SHIRAISHI ◽  
Tosihide H. YOSIDA
Keyword(s):  
Differential Staining ◽  
Human Chromosomes

INFLUENCE OF CYSTEAMINE ON DIFFERENTIAL STAINING OF BUdR-SUBSTITUTED HUMAN CHROMOSOMES

1979 ◽  
Vol 21 (1) ◽  
pp. 145-149 ◽  
Author(s):  
W. Scheid
Keyword(s):  
Visible Light ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Strand Breaks ◽  
Light System

In 5-bromodeoxyuridine (BUdR)-substituted human chromosomes stained with 4′-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.

GIEMSA STAINING OF HETEROCHROMATIC AREAS OF HUMAN CHROMOSOMES FOR IDENTIFICATION

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner
Keyword(s):  
Acetic Acid ◽  
Banding Pattern ◽  
Saline Solution ◽  
Simple Technique ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Dna Denaturation ◽  
Quinacrine Fluorescence ◽  
Inexpensive Procedure ◽  
Distinct Banding Pattern

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.

Accidental Modification of Attwood's Stain on Decalcified Preparations, Yielding Improved Differentiation between Woven and Lamellar Bone

2001 ◽  
Vol 4 (1) ◽  
pp. 32-36 ◽  
Author(s):  
S. Lee ◽  
R. Stocks ◽  
D.J. de Sa
Keyword(s):  
Osteogenesis Imperfecta ◽  
Potassium Permanganate ◽  
Periodic Acid ◽  
Previous Method ◽  
Matrix Proteins ◽  
Differential Staining ◽  
Lamellar Bone ◽  
Woven Bone ◽  
Cartilaginous Matrix ◽  
Original Application

In an earlier report, a modification of the Attwood's stain was used to study sections of decalcified bone and the procedure aided in the distinction between lamellar and woven bone. Lamellar bone showed preferential affinity for phloxine (red) yet resisted differentiation with tartrazine (yellow), while woven bone stained well with tartrazine. In addition, osteoid seams were identified by their affinity for tartrazine. Differential staining was abolished by pretreatment with periodic acid. Recently, accidental use of potassium permanganate in an acidified medium of pH 1.94, instead of pH 6.44, yielded unexpected results with a much sharper, consistent distinction between woven and lamellar bone. This probably reflects changes in the noncollagenous, interfibrillary matrix proteins of bone, but the exact reason(s) is not known. In our view, this serendipitous modification represents an advance over the previous method, and has allowed us to study the structure of bone in some of the systemic disorders, e.g., osteogenesis imperfecta, with greater ease than before. This method does, however, suffer the disadvantage of interfering with the intensity of the staining of any cartilaginous matrix, and the original application is still of value.

Ag-Nor staining in human chromosomes: Differential staining in normal and leukemic bone-marrow samples

1985 ◽  
Vol 36 (6) ◽  
pp. 647-649 ◽  
Author(s):  
K. C. Arden ◽  
S. Pathak ◽  
L. S. Frankel ◽  
A. Zander
Keyword(s):  
Bone Marrow ◽  
Differential Staining ◽  
Human Chromosomes

An Analysis of Factors for the Differential Staining of Sister Chromatids in Human Chromosomes Using Giemsa

1978 ◽  
Vol 27 ◽  
pp. 81-87
Author(s):  
Reita Nand ◽  
P. K. Ghosh
Keyword(s):  
Conformational Changes ◽  
Proteolytic Enzymes ◽  
Staining Technique ◽  
Differential Staining ◽  
Chromosomal Protein ◽  
Human Chromosomes ◽  
Trypsin Treatment ◽  
Slide Preparation ◽  
Sister Chromatids

Various reagents were tested for the purpose of developing an improved Giemsa staining technique for the differential staining of sister chromatids in human chromosomes. Reagents like acids, bases, buffers, protein denaturants and proteolytic enzymes were all potent inducers of differential staining. The best results were obtained by brief trypsinization followed by extraction of nucleic acids by incubation in hot HCl. There was poor contrast between unifilarly and bifilarly BrdU substituted chromatids in slides from which trypsin treatment was omitted. The method of slide preparation as they affect the spreads of BrdU substituted metaphases were also evaluated. The results support the role of these reagents in the conformational changes and structural lesions of chromosomal protein leading to differential staining.

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