Deoxyribonucleic Acid Content (Feulgen Photometry) and Dry Weight (Interference Microscopy) of Normal and Hypertrophic Heart Muscle Fibres

Nature ◽  
1964 ◽  
Vol 202 (4927) ◽  
pp. 100-101 ◽  
Author(s):  
W. SANDRITTER ◽  
G. SCOMAZZONI
Keyword(s):  
Acid Content ◽  
Heart Muscle ◽  
Deoxyribonucleic Acid ◽  
Dry Weight ◽  
Interference Microscopy ◽  
Muscle Fibres

Composition of Methanospirillum hungatii GP1 during growth on different media

1980 ◽  
Vol 26 (5) ◽  
pp. 577-582 ◽  
Author(s):  
C. Breuil ◽  
G. B. Patel
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Synthetic Medium ◽  
Density Measurement ◽  
Dry Weight ◽  
Weight Basis ◽  
Total Carbohydrate ◽  
Mineral Salts

Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.

scholarly journals Studies on Deoxyribonucleic Acid Content and Cell Count of Human Heart Muscle

1970 ◽  
Vol 11 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Toshiaki MORISHITA ◽  
Rikuro SASAKI ◽  
Shoichi YAMAGATA
Keyword(s):  
Cell Count ◽  
Acid Content ◽  
Heart Muscle ◽  
Human Heart ◽  
Deoxyribonucleic Acid

scholarly journals Determination of Deoxyribonucleic Acid Content of Heart Muscle and Myocardial Growth in Normal Rats

1968 ◽  
Vol 95 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Rikuro Sasaki ◽  
Yasuo Watanabe ◽  
Toshiaki Morishita ◽  
Shoichi Yamagata
Keyword(s):  
Acid Content ◽  
Heart Muscle ◽  
Deoxyribonucleic Acid

scholarly journals The measurement of the deoxyribonucleic acid content of human granulocytes

1972 ◽  
Vol 127 (2) ◽  
pp. 40P-40P ◽  
Author(s):  
M J Blackburn ◽  
T M Andrews ◽  
R W E Watts
Keyword(s):  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Human Granulocytes

scholarly journals Cytological and Biochemical Studies of Anucleolate Xenopus Larvae

1962 ◽  
Vol s3-103 (61) ◽  
pp. 25-35
Author(s):  
H. WALLACE
Keyword(s):  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Nucleic Acid Content ◽  
Tail Bud ◽  
Lethal Mutant ◽  
Cytoplasmic Rna ◽  
Biochemical Studies ◽  
And Function

A larval lethal mutant of Xenopus laevis lacks true nucleoli but possesses analogous intranuclear organelles, here termed blobs, which are smaller and more numerous than nucleoli. Cytochemical tests reveal that blobs (like nucleoli) contain ribonucleic acid (RNA), arginine, and alkaline phosphatase, but probably no Feulgen-positive material. Anucleolate larvae are deficient in cytoplasmic RNA. By biochemical methods the nucleic acid content of anucleolate embryos is found to be normal at the tail-bud stage, but does not increase after this. By the time they hatch, anucleolate larvae are deficient in both RNA and deoxyribonucleic acid (DNA). The implications of this and related mutations on the formation and function of the nucleolus are considered. The term ‘blob’ is justified in that it would be misleading to regard such organelles as nucleoli produced by normally latent organizers.

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