Partial Purification of Cell Growth-Promoting Principles in Adult Tissue Extract

Nature ◽  
1942 ◽  
Vol 150 (3814) ◽  
pp. 660-661 ◽  
Author(s):  
H. WERNER ◽  
L. DOLJANSKI
Keyword(s):  
Cell Growth ◽  
Tissue Extract ◽  
Partial Purification ◽  
Adult Tissue ◽  
Growth Promoting

Effect of water-soluble extract from antler of wapiti (Cervus elaphus) on the growth of fibroblasts

1997 ◽  
Vol 77 (2) ◽  
pp. 343-345 ◽  
Author(s):  
H. H. Sunwoo ◽  
T. Nakano ◽  
J. S. Sim
Keyword(s):  
Growth Factor ◽  
Cell Growth ◽  
Cervus Elaphus ◽  
Water Soluble ◽  
Soluble Extract ◽  
Key Words Growth ◽  
Growth Promoting ◽  
Water Soluble Extract ◽  
Dose Dependent ◽  
Stimulation Of

The water-soluble extracts were prepared from the tip sections of antlers of 4-yr-old wapiti stags, and the effect of extract on the growth of bovine skin fibroblasts in culture was examined. The results showed the presence of growth promoting factor(s) in the antler extract. The stimulation of cell growth was found to be dose dependent (P < 0.05). Key words: Growth factor, antler, wapiti, fibroblast

Putative Mesothelial Cell Growth-Promoting Activity of a Cytoplasmic Protein Expressed by the Mesothelial Cell

Pathobiology ◽  
1989 ◽  
Vol 57 (4) ◽  
pp. 193-197 ◽  
Author(s):  
Adalberto Donna ◽  
Pier-Giacomo Betta ◽  
Maria Fabia Cosimi ◽  
Flavio Robutti ◽  
Domenico Bellingeri ◽  
...  
Keyword(s):  
Cell Growth ◽  
Mesothelial Cell ◽  
Cytoplasmic Protein ◽  
Growth Promoting

Role of small GTP binding proteins in the growth-promoting and antiapoptotic actions of gastrin

2004 ◽  
Vol 287 (3) ◽  
pp. G715-G725 ◽  
Author(s):  
Vinzenz Stepan ◽  
Saravanan Ramamoorthy ◽  
Nonthalee Pausawasdi ◽  
Craig D. Logsdon ◽  
Frederick K. Askari ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Binding Proteins ◽  
Dominant Negative ◽  
Growth And Survival ◽  
Gtp Binding Proteins ◽  
Gtp Binding ◽  
Growth Promoting ◽  
Stimulation Of ◽  
Cell Growth And Survival

G17 has growth promoting and antiapoptotic effects on the AR4–2J pancreatic acinar cell line. We previously reported that whereas MAPK regulates G17-stimulation of AR4–2J cell proliferation, Akt mediates the antiapoptotic action of G17. We examined the signal-transduction pathways mediating G17 stimulation of AR4–2J cell growth and survival. G17 activated the small GTP binding proteins Ras, Rac, Rho, and Cdc42. Transduction of the cells with adenoviral vectors expressing dominant negative Akt, Ras, Rho, and Cdc42 but not dominant negative Rac inhibited AR4–2J cell proliferation and survival. Both exoenzyme C3 from Clostridium botulinum (C3), a toxin known to inactivate Rho, and PD98059, a MAPK inhibitor, reversed G17 inhibition of AR4–2J cell apoptosis. G17 induction of Akt activation was reduced by >60% by both dominant negative Ras and Rho and by 30% by dominant negative Cdc42. In contrast, G17-stimulated MAPK activation was blocked by >80% by dominant negative Ras but not by dominant negative Rho and Cdc42. Similar results were observed in the presence of C3. Dominant negative Rac failed to affect G17 induction of both Akt and MAPK, whereas it inhibited sorbitol by almost 50% but not G17-stimulated activation of p38 kinase. Thus G17 promotes AR4–2J cell growth and survival through the activation of multiple GTP binding proteins, which, in turn, regulate different protein kinase cascades. Whereas Ras activates Akt and MAPK, Rho and Cdc42 appear to regulate Akt and possibly other as yet unidentified kinases mediating the growth-stimulatory actions of G17.

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