scholarly journals Biphasic Regulation of Intracellular Calcium by Gemfibrozil Contributes to Inhibiting L6 Myoblast Differentiation: Implications for Clinical Myotoxicity

2011 ◽  
Vol 24 (2) ◽  
pp. 229-237 ◽  
Author(s):  
Aiming Liu ◽  
Julin Yang ◽  
Frank J. Gonzalez ◽  
Gary Q. Cheng ◽  
Renke Dai
Keyword(s):  
Intracellular Calcium ◽  
Myoblast Differentiation ◽  
L6 Myoblast

scholarly journals Opposite roles of myocardin and atrogin-1 in L6 myoblast differentiation

2013 ◽  
Vol 228 (10) ◽  
pp. 1989-1995 ◽  
Author(s):  
Yulan Jiang ◽  
Pavneet Singh ◽  
Hao Yin ◽  
Yi-Xia Zhou ◽  
Yu Gui ◽  
...  
Keyword(s):  
Myoblast Differentiation ◽  
L6 Myoblast

Integrin-linked kinase is a positive mediator of L6 myoblast differentiation

2003 ◽  
Vol 310 (3) ◽  
pp. 796-803 ◽  
Author(s):  
Mathew G Miller ◽  
Izabela Naruszewicz ◽  
Ashu S Kumar ◽  
Toolsie Ramlal ◽  
Gregory E Hannigan
Keyword(s):  
Myoblast Differentiation ◽  
Integrin Linked Kinase ◽  
L6 Myoblast

scholarly journals Reduction in Intracellular Calcium Levels Inhibits Myoblast Differentiation

2002 ◽  
Vol 277 (32) ◽  
pp. 28942-28947 ◽  
Author(s):  
George A. Porter ◽  
Ryan F. Makuck ◽  
Scott A. Rivkees
Keyword(s):  
Intracellular Calcium ◽  
Myoblast Differentiation

Biphasic concentration dependency of stimulation of myoblast differentiation by somatomedins

1986 ◽  
Vol 250 (5) ◽  
pp. C771-C778 ◽  
Author(s):  
J. R. Florini ◽  
D. Z. Ewton ◽  
S. L. Falen ◽  
J. J. Van Wyk
Keyword(s):  
Growth Factor ◽  
Myoblast Differentiation ◽  
Medium Components ◽  
Low Concentrations ◽  
Concentration Dependency ◽  
Epidermal Growth ◽  
L6 Myoblast ◽  
Stimulation Of ◽  
Insulin Like Growth Factors

It is widely believed that mitogens inhibit in vitro differentiation of myoblasts to form postmitotic myotubes, but we and others have shown that the mitogenic hormones insulin and the insulin-like growth factors (IGFs) stimulate myoblast differentiation. We now report the results of concentration-dependency studies that resolve this disagreement. We found that the IGFs give a biphasic dose-response curve; at low concentrations, there is progressive stimulation of L6 myoblast differentiation; at higher concentrations, there is a progressive decrease. Similar results were obtained with IGF-II and insulin. When differentiation was maximally stimulated (by 1,280 ng/ml insulin), adding rat IGF-II gave decreases in differentiation similar to those reported for other mitogens. Two trivial explanations have been eliminated: stimulation of differentiation (at low concentrations) is not due to enhanced survival or growth of the cells, and inhibition (at higher concentrations) is not a toxic effect. In L6 cells, epidermal growth factor and fibroblast growth factor had no effect on proliferation or differentiation. We conclude that the effects of medium components on myoblast differentiation cannot be generalized to indicate inhibition by all mitogens; depending on the cell lines and concentrations used, certain mitogens may either stimulate or inhibit differentiation.

Ultra-Rapid Freezing of Isolated Skeletal Muscle Fibers

1977 ◽  
Vol 35 ◽  
pp. 576-577
Author(s):  
Joachim R. Sommer ◽  
Nancy R. Wallace
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Granular Material ◽  
Nuclear Envelope ◽  
Intracellular Calcium ◽  
Ruthenium Red ◽  
Threshold Concentration ◽  
Rapid Freezing ◽  
Frog Skeletal Muscle ◽  
Electrical Isolation

After Howell (1) had shown that ruthenium red treatment of fixed frog skeletal muscle caused collapse of the intermediate cisternae of the sarcoplasmic reticulum (SR), forming a pentalaminate structure by obi iterating the SR lumen, we demonstrated that the phenomenon involves the entire SR including the nuclear envelope and that it also occurs after treatment with other cations, including calcium (2,3,4).From these observations we have formulated a hypothesis which states that intracellular calcium taken up by the SR at the end of contraction causes the M rete to collapse at a certain threshold concentration as the first step in a subsequent centrifugal zippering of the free SR toward the junctional SR (JSR). This would cause a) bulk transport of SR contents, such as calcium and granular material (4) into the JSR and, b) electrical isolation of the free SR from the JSR.

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