scholarly journals Opposite roles of myocardin and atrogin-1 in L6 myoblast differentiation

2013 ◽  
Vol 228 (10) ◽  
pp. 1989-1995 ◽  
Author(s):  
Yulan Jiang ◽  
Pavneet Singh ◽  
Hao Yin ◽  
Yi-Xia Zhou ◽  
Yu Gui ◽  
...  
Keyword(s):  
Myoblast Differentiation ◽  
L6 Myoblast

Integrin-linked kinase is a positive mediator of L6 myoblast differentiation

2003 ◽  
Vol 310 (3) ◽  
pp. 796-803 ◽  
Author(s):  
Mathew G Miller ◽  
Izabela Naruszewicz ◽  
Ashu S Kumar ◽  
Toolsie Ramlal ◽  
Gregory E Hannigan
Keyword(s):  
Myoblast Differentiation ◽  
Integrin Linked Kinase ◽  
L6 Myoblast

Biphasic concentration dependency of stimulation of myoblast differentiation by somatomedins

1986 ◽  
Vol 250 (5) ◽  
pp. C771-C778 ◽  
Author(s):  
J. R. Florini ◽  
D. Z. Ewton ◽  
S. L. Falen ◽  
J. J. Van Wyk
Keyword(s):  
Growth Factor ◽  
Myoblast Differentiation ◽  
Medium Components ◽  
Low Concentrations ◽  
Concentration Dependency ◽  
Epidermal Growth ◽  
L6 Myoblast ◽  
Stimulation Of ◽  
Insulin Like Growth Factors

It is widely believed that mitogens inhibit in vitro differentiation of myoblasts to form postmitotic myotubes, but we and others have shown that the mitogenic hormones insulin and the insulin-like growth factors (IGFs) stimulate myoblast differentiation. We now report the results of concentration-dependency studies that resolve this disagreement. We found that the IGFs give a biphasic dose-response curve; at low concentrations, there is progressive stimulation of L6 myoblast differentiation; at higher concentrations, there is a progressive decrease. Similar results were obtained with IGF-II and insulin. When differentiation was maximally stimulated (by 1,280 ng/ml insulin), adding rat IGF-II gave decreases in differentiation similar to those reported for other mitogens. Two trivial explanations have been eliminated: stimulation of differentiation (at low concentrations) is not due to enhanced survival or growth of the cells, and inhibition (at higher concentrations) is not a toxic effect. In L6 cells, epidermal growth factor and fibroblast growth factor had no effect on proliferation or differentiation. We conclude that the effects of medium components on myoblast differentiation cannot be generalized to indicate inhibition by all mitogens; depending on the cell lines and concentrations used, certain mitogens may either stimulate or inhibit differentiation.

scholarly journals Epicatechin elicits MyoD-dependent myoblast differentiation and myogenic conversion of fibroblasts

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0175271 ◽  
Author(s):  
Sang-Jin Lee ◽  
Young-Eun Leem ◽  
Ga-Yeon Go ◽  
Younhee Choi ◽  
Yoo Jin Song ◽  
...  
Keyword(s):  
Myoblast Differentiation

scholarly journals MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 423
Author(s):  
Genxi Zhang ◽  
Mingliang He ◽  
Pengfei Wu ◽  
Xinchao Zhang ◽  
Kaizhi Zhou ◽  
...  
Keyword(s):  
Muscle Growth ◽  
Luciferase Reporter ◽  
Myoblast Differentiation ◽  
Regulation Mechanism ◽  
Rna Seq ◽  
Myostatin Gene ◽  
Primary Myoblasts ◽  
Chicken Muscle ◽  
Proliferation And Differentiation ◽  
Myoblast Proliferation

microRNAs play an important role in the growth and development of chicken embryos, including the regulation of skeletal muscle genesis, myoblast proliferation, differentiation, and apoptosis. Our previous RNA-seq studies showed that microRNA-27b-3p (miR-27b-3p) might play an important role in regulating the proliferation and differentiation of chicken primary myoblasts (CPMs). However, the mechanism of miR-27b-3p regulating the proliferation and differentiation of CPMs is still unclear. In this study, the results showed that miR-27b-3p significantly promoted the proliferation of CPMs and inhibited the differentiation of CPMs. Then, myostatin (MSTN) was confirmed to be the target gene of miR-27b-3p by double luciferase reporter assay, RT-qPCR, and Western blot. By overexpressing and interfering with MSTN expression in CPMs, the results showed that overexpression of MSTN significantly inhibited the proliferation and differentiation of CPMs. In contrast, interference of MSTN expression had the opposite effect. This study showed that miR-27b-3p could promote the proliferation of CPMs by targeting MSTN. Interestingly, both miR-27b-3p and MSTN can inhibit the differentiation of CPMs. These results provide a theoretical basis for further understanding the function of miR-27b-3p in chicken and revealing its regulation mechanism on chicken muscle growth.

scholarly journals The Leucine Catabolite and Dietary Supplement β-Hydroxy-β-Methyl Butyrate (HMB) as an Epigenetic Regulator in Muscle Progenitor Cells

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 512
Author(s):  
Virve Cavallucci ◽  
Giovambattista Pani
Keyword(s):  
Histone Acetylation ◽  
Dietary Supplement ◽  
Hdac Inhibitor ◽  
Muscle Wasting ◽  
C2c12 Cells ◽  
Myoblast Differentiation ◽  
Epigenetic Mark ◽  
Epigenetic Regulator ◽  
Methyl Butyrate

β-Hydroxy-β-Methyl Butyrate (HMB) is a natural catabolite of leucine deemed to play a role in amino acid signaling and the maintenance of lean muscle mass. Accordingly, HMB is used as a dietary supplement by sportsmen and has shown some clinical effectiveness in preventing muscle wasting in cancer and chronic lung disease, as well as in age-dependent sarcopenia. However, the molecular cascades underlying these beneficial effects are largely unknown. HMB bears a significant structural similarity with Butyrate and β-Hydroxybutyrate (βHB), two compounds recognized for important epigenetic and histone-marking activities in multiple cell types including muscle cells. We asked whether similar chromatin-modifying actions could be assigned to HMB as well. Exposure of murine C2C12 myoblasts to millimolar concentrations of HMB led to an increase in global histone acetylation, as monitored by anti-acetylated lysine immunoblotting, while preventing myotube differentiation. In these effects, HMB resembled, although with less potency, the histone deacetylase (HDAC) inhibitor Sodium Butyrate. However, initial studies did not confirm a direct inhibitory effect of HMB on HDACs in vitro. β-Hydroxybutyrate, a ketone body produced by the liver during starvation or intense exercise, has a modest effect on histone acetylation of C2C12 cells or in vitro HDAC inhibitor activities, and, unlike Butyrate and HMB, did not interfere with myotube formation in a myoblast differentiation assay. Instead, βHB dramatically increased lysine β-hydroxybutyrylation (Kbhb) of histone tails, an epigenetic mark associated with fasting responses and muscle catabolic states. However, when C2C12 cells were exposed to βHB in the presence of equimolar HMB this chromatin modification was drastically reduced, pointing to a role for HMB in attenuating ketosis-associated muscle wasting. In conclusion, while their mechanistic underpinnings remain to be clarified, these preliminary observations highlight novel and potentially important activities of HMB as an epigenetic regulator and βHB antagonist in muscle precursor cells, to be further explored in their biomedical implications.

scholarly journals Fast photocurable thiol-ene elastomers with tunable biodegradability, mechanical and surface properties enhance myoblast differentiation and contractile function

2021 ◽  
Vol 6 (7) ◽  
pp. 2120-2133
Author(s):  
Mohamed Alaa Mohamed ◽  
Aref Shahini ◽  
Nika Rajabian ◽  
Julia Caserto ◽  
Ahmed M.A. El-Sokkary ◽  
...  
Keyword(s):  
Surface Properties ◽  
Contractile Function ◽  
Myoblast Differentiation
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