scholarly journals Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-CausingPlasmodiumParasites Based on Enzyme Activity Measurement

ACS Nano ◽  
2012 ◽  
Vol 6 (12) ◽  
pp. 10676-10683 ◽  
Author(s):  
Sissel Juul ◽  
Christine J. F. Nielsen ◽  
Rodrigo Labouriau ◽  
Amit Roy ◽  
Cinzia Tesauro ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Droplet Microfluidics ◽  
Quantitative Detection ◽  
Activity Measurement ◽  
Highly Sensitive ◽  
Microfluidics Platform

Rapid, highly sensitive and quantitative detection of interleukin 6 based on SERS magnetic immunoassay

2021 ◽  
Vol 13 (15) ◽  
pp. 1823-1831
Author(s):  
Xiaomei Wang ◽  
Li Ma ◽  
Shijiao Sun ◽  
Tingwei Liu ◽  
Hao Zhou ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Detection Time ◽  
Highly Sensitive ◽  
Sandwich Method

We have developed a SERS magnetic immunoassay method based on the principle of sandwich method for rapid and quantitative detection of IL-6. The developed SERS method has the advantages of high sensitivity and detection time is only 15 min.

Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

An ultra high-efficiency droplet microfluidics platform using automatically synchronized droplet pairing and merging

Lab on a Chip ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 3948-3959 ◽  
Author(s):  
Han Zhang ◽  
Adrian R. Guzman ◽  
Jose A. Wippold ◽  
Yuwen Li ◽  
Jing Dai ◽  
...  
Keyword(s):  
High Efficiency ◽  
Droplet Microfluidics ◽  
Microfluidics Platform

The integrated droplet platform combines curved microstructures that allow high-efficiency (99.9%) reflow of droplets and a droplet cleaving that automatically synchronizes paired droplets enabling high-efficiency (99.9%) downstream merging.

scholarly journals Highly sensitive and quantitative detection of BCR-ABL kinase domain mutations by ligation PCR

Leukemia ◽  
2008 ◽  
Vol 22 (12) ◽  
pp. 2288-2291 ◽  
Author(s):  
O Pelz-Ackermann ◽  
M Cross ◽  
H Pfeifer ◽  
M Deininger ◽  
S-Y Wang ◽  
...  
Keyword(s):  
Kinase Domain ◽  
Quantitative Detection ◽  
Abl Kinase ◽  
Highly Sensitive ◽  
Kinase Domain Mutations

Highly sensitive and quantitative detection of EGFR T790M mutationin tumor samples by nanofluidic digital PCR.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. 11091-11091
Author(s):  
Eiji Iwama ◽  
Koichi Takayama ◽  
Taishi Harada ◽  
Isamu Okamoto ◽  
Eishi Baba ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Quantitative Detection ◽  
Highly Sensitive ◽  
Egfr T790m

A droplet microfluidics platform for rapid microalgal growth and oil production analysis

2016 ◽  
Vol 113 (8) ◽  
pp. 1691-1701 ◽  
Author(s):  
Hyun Soo Kim ◽  
Adrian R. Guzman ◽  
Hem R. Thapa ◽  
Timothy P. Devarenne ◽  
Arum Han
Keyword(s):  
Oil Production ◽  
Droplet Microfluidics ◽  
Production Analysis ◽  
Microfluidics Platform

scholarly journals Quantitative Detection of Listeria monocytogenes and Listeria innocua by Real-Time PCR: Assessment of hly, iap, and lin02483 Targets and AmpliFluor Technology

2004 ◽  
Vol 70 (3) ◽  
pp. 1366-1377 ◽  
Author(s):  
David Rodr�guez-L�zaro ◽  
Marta Hern�ndez ◽  
Mariela Scortti ◽  
Teresa Esteve ◽  
Jos� A. V�zquez-Boland ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Real Time Pcr ◽  
Target Genes ◽  
Dynamic Range ◽  
Significant Proportion ◽  
Quantitative Detection ◽  
Target Sequence ◽  
Food Borne ◽  
Highly Sensitive

ABSTRACT We developed and assessed real-time PCR (RTi-PCR) assays for the detection and quantification of the food-borne pathogen Listeria monocytogenes and the closely related nonpathogenic species L. innocua. The target genes were hly and iap for L. monocytogenes and lin02483 for L. innocua. The assays were 100% specific, as determined with 100 Listeria strains and 45 non-Listeria strains, and highly sensitive, with detection limits of one target molecule in 11 to 56% of the reactions with purified DNA and 3 CFU in 56 to 89% of the reactions with bacterial suspensions. Quantification was possible over a 5-log dynamic range, with a limit of 15 target molecules and R 2 values of >0.996. There was an excellent correspondence between the predicted and the actual numbers of CFU in the samples (deviations of <23%). The hly-based assay accurately quantified L. monocytogenes in all of the samples tested. The iap-based assay, in contrast, was unsuitable for quantification purposes, underestimating the bacterial counts by 3 to 4 log units in a significant proportion of the samples due to serovar-related target sequence variability. The combination of the two assays enabled us to classify L. monocytogenes isolates into one of the two major phylogenetic divisions of the species, I and II. We also assessed the new AmpliFluor technology for the quantitative detection of L. monocytogenes by RTi-PCR. The performance of this system was similar to that of the TaqMan system, although the former system was slightly less sensitive (detection limit of 15 molecules in 45% of the reactions) and had a higher quantification limit (60 molecules).

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