Experimental Evidence of Plasmophores: Plasmon-Directed Polarized Emission from Gold Nanorod–Fluorophore Hybrid Nanostructures

Nano Letters ◽  
2011 ◽  
Vol 11 (6) ◽  
pp. 2296-2303 ◽  
Author(s):  
Tian Ming ◽  
Lei Zhao ◽  
Huanjun Chen ◽  
Kat Choi Woo ◽  
Jianfang Wang ◽  
...  
Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 444
Author(s):  
Xuemeng Li ◽  
Yingshuting Wang ◽  
Quanying Fu ◽  
Yangyang Wang ◽  
Dongxu Ma ◽  
...  

Recently, biosensing based on weak coupling in plasmon-emitter hybrid nanostructures exhibits the merits of simplicity and high sensitivity, and attracts increasing attention as an emerging nano-sensor. In this study, we propose an innovative plasmon-regulated fluorescence resonance energy transfer (plasmon-regulated FRET) sensing strategy based on a plasmon-emitter hybrid nanostructure of gold nanorod-quantum dots (Au NR-QDs) by partially modifying QDs onto the surfaces of Au NRs. The Au NR-QDs showed good sensitivity and reversibility against refractive index change. We successfully employed the Au NR-QDs to fabricate nano-sensors for detecting a cancer biomarker of alpha fetoprotein with a limit of detection of 0.30 ng/mL, which displays that the sensitivity of the Au NR-QDs nano-sensor was effectively improved compared with the Au NRs based plasmonic sensing. Additionally, to demonstrate the universality of the plasmon-regulated FRET sensing strategy, another plasmon-emitter hybrid nano-sensor of Au nano-prism-quantum dots (Au NP-QDs) were constructed and applied for detecting a myocardial infarction biomarker of cardiac troponin I. It was first reported that the change of absorption spectra of plasmonic structure in a plasmon-emitter hybrid nanostructure was employed for analytes detection. The plasmon-regulated FRET sensing strategy described herein has potential utility to develop general sensing platforms for chemical and biological analysis.


2013 ◽  
Vol 1 (1) ◽  
pp. 28 ◽  
Author(s):  
Jiafang Li ◽  
Honglian Guo ◽  
Zhi-Yuan Li

Nanoscale ◽  
2017 ◽  
Vol 9 (23) ◽  
pp. 7750-7754 ◽  
Author(s):  
Linlin Song ◽  
Qiao Jiang ◽  
Jianbing Liu ◽  
Na Li ◽  
Qing Liu ◽  
...  

NANO ◽  
2014 ◽  
Vol 09 (03) ◽  
pp. 1450035
Author(s):  
LU ZHANG ◽  
YAN-JUAN TANG ◽  
YAN-JIE GUO ◽  
JIAN-JUN LUO ◽  
GUI-MIN SUN ◽  
...  

A novel core–shell hybrid nanostructure was constructed by employing gold nanorod ( AuNR ) combined with rhodamine B (RB) as a core and silica as a shell. The poly(sodium 4-styrenesulfonate) (PSS), a negatively charged polyelectrolyte, played the role of linker to electrostatically trap RB on AuNRs . Due to the fluorescence spectral overlap between RB and AuNRs at 560 nm, the red fluorescence and enhanced green fluorescence of the hybrid nanostructures were observed obviously, which is capable for dual-color labeling. To reduce toxic side effects of AuNRs , silica was coated on AuNRs as a shell to fabricate the novel core–shell hybrid nanostructure function as a dual-color labeling for cancer-cell imaging. The fabricated composite structures were characterized by transmission electron microscopy (TEM), absorption spectrum, fluorescence spectrum, zeta potential measurements and laser scanning confocal microscope (LSCM). The experiment results confirmed that the obtained hybrid nanostructures provided excellent photostability, biocompatibility and active surface for further biological functionalization. The novel composite structures may have great potential application in cell multicolor labeling and imaging instead of traditional fluorescent dyes.


2008 ◽  
Vol 130 (21) ◽  
pp. 6692-6693 ◽  
Author(s):  
Weihai Ni ◽  
Zhi Yang ◽  
Huanjun Chen ◽  
Li Li ◽  
Jianfang Wang

2019 ◽  
Vol 42 ◽  
Author(s):  
Olya Hakobyan ◽  
Sen Cheng

Abstract We fully support dissociating the subjective experience from the memory contents in recognition memory, as Bastin et al. posit in the target article. However, having two generic memory modules with qualitatively different functions is not mandatory and is in fact inconsistent with experimental evidence. We propose that quantitative differences in the properties of the memory modules can account for the apparent dissociation of recollection and familiarity along anatomical lines.


1997 ◽  
Vol 161 ◽  
pp. 437-442
Author(s):  
Salvatore Di Bernardo ◽  
Romana Fato ◽  
Giorgio Lenaz

AbstractOne of the peculiar aspects of living systems is the production and conservation of energy. This aspect is provided by specialized organelles, such as the mitochondria and chloroplasts, in developed living organisms. In primordial systems lacking specialized enzymatic complexes the energy supply was probably bound to the generation and maintenance of an asymmetric distribution of charged molecules in compartmentalized systems. On the basis of experimental evidence, we suggest that lipophilic quinones were involved in the generation of this asymmetrical distribution of charges through vectorial redox reactions across lipid membranes.


Author(s):  
Michael T. Bucek ◽  
Howard J. Arnott

It is believed by the authors, with supporting experimental evidence, that as little as 0.5°, or less, knife clearance angle may be a critical factor in obtaining optimum quality ultrathin sections. The degree increments located on the knife holder provides the investigator with only a crude approximation of the angle at which the holder is set. With the increments displayed on the holder one cannot set the clearance angle precisely and reproducibly. The ability to routinely set this angle precisely and without difficulty would obviously be of great assistance to the operator. A device has been contrived to aid the investigator in precisely setting the clearance angle. This device is relatively simple and is easily constructed. It consists of a light source and an optically flat, front surfaced mirror with a minute black spot in the center. The mirror is affixed to the knife by placing it permanently on top of the knife holder.


Author(s):  
H. Mohri

In 1959, Afzelius observed the presence of two rows of arms projecting from each outer doublet microtubule of the so-called 9 + 2 pattern of cilia and flagella, and suggested a possibility that the outer doublet microtubules slide with respect to each other with the aid of these arms during ciliary and flagellar movement. The identification of the arms as an ATPase, dynein, by Gibbons (1963)strengthened this hypothesis, since the ATPase-bearing heads of myosin molecules projecting from the thick filaments pull the thin filaments by cross-bridge formation during muscle contraction. The first experimental evidence for the sliding mechanism in cilia and flagella was obtained by examining the tip patterns of molluscan gill cilia by Satir (1965) who observed constant length of the microtubules during ciliary bending. Further evidence for the sliding-tubule mechanism was given by Summers and Gibbons (1971), using trypsin-treated axonemal fragments of sea urchin spermatozoa. Upon the addition of ATP, the outer doublets telescoped out from these fragments and the total length reached up to seven or more times that of the original fragment. Thus, the arms on a certain doublet microtubule can walk along the adjacent doublet when the doublet microtubules are disconnected by digestion of the interdoublet links which connect them with each other, or the radial spokes which connect them with the central pair-central sheath complex as illustrated in Fig. 1. On the basis of these pioneer works, the sliding-tubule mechanism has been established as one of the basic mechanisms for ciliary and flagellar movement.


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