scholarly journals Ion Channel Probes for Scanning Ion Conductance Microscopy

Langmuir ◽  
2014 ◽  
Vol 30 (50) ◽  
pp. 15351-15355 ◽  
Author(s):  
Yi Zhou ◽  
Leonard K. Bright ◽  
Wenqing Shi ◽  
Craig A. Aspinwall ◽  
Lane A. Baker
Keyword(s):  
Ion Channel ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

Membrane patches as ion channel probes for scanning ion conductance microscopy

2016 ◽  
Vol 193 ◽  
pp. 81-97 ◽  
Author(s):  
Wenqing Shi ◽  
Yuhan Zeng ◽  
Lushan Zhou ◽  
Yucheng Xiao ◽  
Theodore R. Cummins ◽  
...  
Keyword(s):  
Ion Channels ◽  
Ion Channel ◽  
Single Channel ◽  
Donor Cell ◽  
Bk Channels ◽  
Ion Conductance ◽  
Current Time ◽  
Scanning Ion Conductance Microscopy ◽  
Close Proximity ◽  
Membrane Patch

We describe dual-barrel ion channel probes (ICPs), which consist of an open barrel and a barrel with a membrane patch directly excised from a donor cell. When incorporated with scanning ion conductance microscopy (SICM), the open barrel (SICM barrel) serves to measure the distance-dependent ion current for non-invasive imaging and positioning of the probe in the same fashion of traditional SICM. The second barrel with the membrane patch supports ion channels of interest and was used to investigate ion channel activities. To demonstrate robust probe control with the dual-barrel ICP-SICM probe and verify that the two barrels are independently addressable, current–distance characteristics (approach curves) were obtained with the SICM barrel and simultaneous, current–time (I–T) traces were recorded with the ICP barrel. To study the influence that the distance between ligand-gated ion channels (i.e., large conductance Ca2+-activated K+ channels/BK channels) and the ligand source (i.e., Ca2+ source) has on channel activations, ion channel activities were recorded at two fixed probe–substrate distances (Dps) with the ICP barrel. The two fixed positions were determined from approach curves acquired with the SICM barrel. One position was defined as the “In-control” position, where the probe was in close proximity to the ligand source; the second position was defined as the “Far” position, where the probe was retracted far away from the ligand source. Our results confirm that channel activities increased dramatically with respect to both open channel probability and single channel current when the probe was near the ligand source, as opposed to when the probe was far away from the ligand source.

scholarly journals Characterization of Membrane Patch-Ion Channel Probes for Scanning Ion Conductance Microscopy

Small ◽  
2017 ◽  
Vol 14 (18) ◽  
pp. 1702945 ◽  
Author(s):  
Wenqing Shi ◽  
Yuhan Zeng ◽  
Cheng Zhu ◽  
Yucheng Xiao ◽  
Theodore R. Cummins ◽  
...  
Keyword(s):  
Ion Channel ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Patch

scholarly journals Cell stiffness and ROS level alterations in living neurons mediated by β-amyloid oligomers measured by scanning ion-conductance microscopy.

2021 ◽  
Vol 27 (S1) ◽  
pp. 500-502
Author(s):  
Oleg Suchalko ◽  
Roman Timoshenko ◽  
Alexander Vaneev ◽  
Vasilii Kolmogorov ◽  
Nikita Savin ◽  
...  
Keyword(s):  
Cell Stiffness ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Amyloid Oligomers ◽  
Β Amyloid ◽  
Living Neurons

scholarly journals Nanoscale Cell Membrane Fluctuations Measured by Scanning Ion Conductance Microscopy

2013 ◽  
Vol 104 (2) ◽  
pp. 317a
Author(s):  
Yusuke Mizutani ◽  
Zen Ishikura ◽  
Myung-Hoon Choi ◽  
Sang-Joon Cho ◽  
Takaharu Okajima
Keyword(s):  
Cell Membrane ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Fluctuations

scholarly journals Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization

2017 ◽  
Vol 114 (9) ◽  
pp. 2395-2400 ◽  
Author(s):  
Umesh Vivekananda ◽  
Pavel Novak ◽  
Oscar D. Bello ◽  
Yuri E. Korchev ◽  
Shyam S. Krishnakumar ◽  
...  
Keyword(s):  
Potassium Channels ◽  
Calcium Influx ◽  
Digital Transmission ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Spike Shape ◽  
Presynaptic Spike ◽  
Synaptic Boutons ◽  
Episodic Ataxia Type

Although action potentials propagate along axons in an all-or-none manner, subthreshold membrane potential fluctuations at the soma affect neurotransmitter release from synaptic boutons. An important mechanism underlying analog–digital modulation is depolarization-mediated inactivation of presynaptic Kv1-family potassium channels, leading to action potential broadening and increased calcium influx. Previous studies have relied heavily on recordings from blebs formed after axon transection, which may exaggerate the passive propagation of somatic depolarization. We recorded instead from small boutons supplied by intact axons identified with scanning ion conductance microscopy in primary hippocampal cultures and asked how distinct potassium channels interact in determining the basal spike width and its modulation by subthreshold somatic depolarization. Pharmacological or genetic deletion of Kv1.1 broadened presynaptic spikes without preventing further prolongation by brief depolarizing somatic prepulses. A heterozygous mouse model of episodic ataxia type 1 harboring a dominant Kv1.1 mutation had a similar broadening effect on basal spike shape as deletion of Kv1.1; however, spike modulation by somatic prepulses was abolished. These results argue that the Kv1.1 subunit is not necessary for subthreshold modulation of spike width. However, a disease-associated mutant subunit prevents the interplay of analog and digital transmission, possibly by disrupting the normal stoichiometry of presynaptic potassium channels.

Scanning Ion Conductance Microscopy for High-Resolution Topography of Soft Samples Including Live Cells

2011 ◽  
Vol 17 (S2) ◽  
pp. 236-237
Author(s):  
G De Filippi ◽  
C Moore
Keyword(s):  
High Resolution ◽  
Live Cells ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Soft Samples

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

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