scholarly journals Characterization of Membrane Patch-Ion Channel Probes for Scanning Ion Conductance Microscopy

Small ◽  
2017 ◽  
Vol 14 (18) ◽  
pp. 1702945 ◽  
Author(s):  
Wenqing Shi ◽  
Yuhan Zeng ◽  
Cheng Zhu ◽  
Yucheng Xiao ◽  
Theodore R. Cummins ◽  
...  
Keyword(s):  
Ion Channel ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Patch

Membrane patches as ion channel probes for scanning ion conductance microscopy

2016 ◽  
Vol 193 ◽  
pp. 81-97 ◽  
Author(s):  
Wenqing Shi ◽  
Yuhan Zeng ◽  
Lushan Zhou ◽  
Yucheng Xiao ◽  
Theodore R. Cummins ◽  
...  
Keyword(s):  
Ion Channels ◽  
Ion Channel ◽  
Single Channel ◽  
Donor Cell ◽  
Bk Channels ◽  
Ion Conductance ◽  
Current Time ◽  
Scanning Ion Conductance Microscopy ◽  
Close Proximity ◽  
Membrane Patch

We describe dual-barrel ion channel probes (ICPs), which consist of an open barrel and a barrel with a membrane patch directly excised from a donor cell. When incorporated with scanning ion conductance microscopy (SICM), the open barrel (SICM barrel) serves to measure the distance-dependent ion current for non-invasive imaging and positioning of the probe in the same fashion of traditional SICM. The second barrel with the membrane patch supports ion channels of interest and was used to investigate ion channel activities. To demonstrate robust probe control with the dual-barrel ICP-SICM probe and verify that the two barrels are independently addressable, current–distance characteristics (approach curves) were obtained with the SICM barrel and simultaneous, current–time (I–T) traces were recorded with the ICP barrel. To study the influence that the distance between ligand-gated ion channels (i.e., large conductance Ca2+-activated K+ channels/BK channels) and the ligand source (i.e., Ca2+ source) has on channel activations, ion channel activities were recorded at two fixed probe–substrate distances (Dps) with the ICP barrel. The two fixed positions were determined from approach curves acquired with the SICM barrel. One position was defined as the “In-control” position, where the probe was in close proximity to the ligand source; the second position was defined as the “Far” position, where the probe was retracted far away from the ligand source. Our results confirm that channel activities increased dramatically with respect to both open channel probability and single channel current when the probe was near the ligand source, as opposed to when the probe was far away from the ligand source.

scholarly journals Ion Channel Probes for Scanning Ion Conductance Microscopy

Langmuir ◽  
2014 ◽  
Vol 30 (50) ◽  
pp. 15351-15355 ◽  
Author(s):  
Yi Zhou ◽  
Leonard K. Bright ◽  
Wenqing Shi ◽  
Craig A. Aspinwall ◽  
Lane A. Baker
Keyword(s):  
Ion Channel ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

Characterization of tip size and geometry of the pipettes used in scanning ion conductance microscopy

Micron ◽  
2016 ◽  
Vol 83 ◽  
pp. 11-18 ◽  
Author(s):  
Elisabetta Tognoni ◽  
Paolo Baschieri ◽  
Cesare Ascoli ◽  
Monica Pellegrini ◽  
Mario Pellegrino
Keyword(s):  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

scholarly journals The nanomorphology of cell surfaces of adhered osteoblasts

2021 ◽  
Vol 12 ◽  
pp. 242-256
Author(s):  
Christian Voelkner ◽  
Mirco Wendt ◽  
Regina Lange ◽  
Max Ulbrich ◽  
Martina Gruening ◽  
...  
Keyword(s):  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Fluctuations ◽  
Power Spectral ◽  
Power Spectral Densities ◽  
Membrane Protrusions ◽  
Fluctuation Dynamics ◽  
And Migration ◽  
Two Parameters

The functionality of living cells is inherently linked to subunits with dimensions ranging from several micrometers down to the nanometer scale. The cell surface plays a particularly important role. Electric signaling, including information processing, takes place at the membrane, as well as adhesion and contact. For osteoblasts, adhesion and spreading are crucial processes with regard to bone implants. Here we present a comprehensive characterization of the 3D nanomorphology of living, as well as fixed, osteoblastic cells using scanning ion conductance microscopy (SICM), which is a nanoprobing method that largely avoids mechanical perturbations. Dynamic ruffles are observed, manifesting themselves in characteristic membrane protrusions. They contribute to the overall surface corrugation, which we systematically study by introducing the relative 3D excess area as a function of the projected adhesion area. A clear anticorrelation between the two parameters is found upon analysis of ca. 40 different cells on glass and on amine-covered surfaces. At the rim of lamellipodia, characteristic edge heights between 100 and 300 nm are observed. Power spectral densities of membrane fluctuations show frequency-dependent decay exponents with absolute values greater than 2 on living osteoblasts. We discuss the capability of apical membrane features and fluctuation dynamics in aiding the assessment of adhesion and migration properties on a single-cell basis.

BIOPHYSICAL APPLICATIONS OF SCANNING ION CONDUCTANCE MICROSCOPY (SICM)

2012 ◽  
Vol 26 (05) ◽  
pp. 1130003 ◽  
Author(s):  
FRANKLIN ANARIBA ◽  
JOON HYUNG ANH ◽  
GOO-EUN JUNG ◽  
NAM-JOON CHO ◽  
SANG-JOON CHO
Keyword(s):  
Chemical Properties ◽  
Live Cells ◽  
Cell Dynamics ◽  
Fundamental Physics ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Patch Clamp Electrophysiology ◽  
And Function ◽  
Physical And Chemical

Scanning probe microscopy (SPM) techniques represent one of the most promising approaches to probe the physical and chemical properties of nanoscale materials. The growing convergence of physics and biology has demanded nanotechnology tools to understand the fundamental physics of biological systems. Despite the advantages of SPM techniques, there have been challenges with its application to characterization of biological specimens. In recent times, the development of one class of SPM technique, scanning ion conductance microscopy (SICM), has overcome these limitations and enabled noninvasive, nanoscale investigation of live cells. In this review article, we present the theory behind the SICM operating principles and data modeling. Based on this framework, we discuss recent research advances where the SICM technique has proven technically superior. SICM applications discussed herein include imaging of cell topography, monitoring of live cell dynamics, mechanical stimulation of live cells, and surface patterning. Additional findings on the combination of SICM with other SPM techniques as well as patch clamp electrophysiology are presented in the context of building integrated knowledge on the structure and function of live cells. In summary, SICM bridges physics and biology to enable a range of important biomedical applications.

Characterization of a Novel Light Source for Simultaneous Optical and Scanning Ion Conductance Microscopy

2002 ◽  
Vol 74 (11) ◽  
pp. 2612-2616 ◽  
Author(s):  
Andreas Bruckbauer ◽  
Liming Ying ◽  
Alison M. Rothery ◽  
Yuri E. Korchev ◽  
David Klenerman
Keyword(s):  
Light Source ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

Characterization of AC mode scanning ion-conductance microscopy

2001 ◽  
Vol 90 (1) ◽  
pp. 13-19 ◽  
Author(s):  
David Pastré ◽  
Hideki Iwamoto ◽  
Jie Liu ◽  
Gabor Szabo ◽  
Zhifeng Shao
Keyword(s):  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

scholarly journals Fabrication and Characterization of Dual Function Nanoscale pH-Scanning Ion Conductance Microscopy (SICM) Probes for High Resolution pH Mapping

2013 ◽  
Vol 85 (17) ◽  
pp. 8070-8074 ◽  
Author(s):  
Binoy Paulose Nadappuram ◽  
Kim McKelvey ◽  
Rehab Al Botros ◽  
Alex W. Colburn ◽  
Patrick R. Unwin
Keyword(s):  
High Resolution ◽  
Dual Function ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Fabrication And Characterization
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