Evidence of the Synergetic Role of Charged Species and Atomic Oxygen in the Molecular Etching of PTFE Surfaces for Hydrophobic Surface Synthesis†

Langmuir ◽  
2010 ◽  
Vol 26 (21) ◽  
pp. 16503-16509 ◽  
Author(s):  
Nicolas Vandencasteele ◽  
Bérangère Broze ◽  
Stéphanie Collette ◽  
Caroline De Vos ◽  
Pascal Viville ◽  
...  
Keyword(s):  
Atomic Oxygen ◽  
Hydrophobic Surface ◽  
Charged Species

Citrate chelation as a potential mechanism against aluminum toxicity in cells: the role of calmodulin

1985 ◽  
Vol 63 (11) ◽  
pp. 1167-1175 ◽  
Author(s):  
Charles G. Suhayda ◽  
Alfred Haug
Keyword(s):  
Circular Dichroism ◽  
Conceptual Understanding ◽  
Aluminum Toxicity ◽  
Hydrophobic Surface ◽  
Native Structure ◽  
Calcium Calmodulin Dependent ◽  
Α Helix ◽  
Kinetics Of ◽  
Circular Dichroïsm

At a molar excess of [citrate]/[aluminum], this organic acid can protect calmodulin from aluminum binding if the metal is presented to the protein in stoichiometric micromolar quantities, as judged by fluorescence and circular dichroism spectroscopy. Similar citrate concentrations are also capable of fully restoring calmodulin's hydrophobic surface exposure to that of the native protein when calmodulin was initially damaged by aluminum binding. Fluoride anions are equally effective in restoring calmodulin's native structure as determined by fluorescence spectroscopy. Measurements of the kinetics of citrate-mediated aluminum removal also indicated that the metal ions are completely removed from calmodulin, consistent with results derived from atomic absorption experiments. On the other hand, results from circular dichroism studies indicated that citrate-mediated aluminum removal from calmodulin can only partially restore the α-helix content to that originally present in apocalmodulin or in calcium–calmodulin, dependent upon the absence or presence of calcium ions. The results that chelators like citrate can protect calmodulin from aluminum injury may provide a conceptual understanding of physiological observations regarding aluminum-tolerant plant species which are generally rich in certain organic acids.

scholarly journals The role of BamA in the biogenesis of beta-barrel membrane proteins

2014 ◽  
Vol 70 (a1) ◽  
pp. C578-C578
Author(s):  
Nicholas Noinaj ◽  
Adam Kuszak ◽  
Curtis Balusek ◽  
JC Gumbart ◽  
Petra Lukacik ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Crystal Structures ◽  
Outer Membrane Proteins ◽  
Hydrophobic Surface ◽  
Md Simulations ◽  
Structural Features ◽  
Bam Complex ◽  
Beta Barrel

Beta-barrel membrane proteins are essential for nutrient import, signaling, motility, and survival. In Gram-negative bacteria, the beta-barrel assembly machinery (BAM) complex is responsible for the biogenesis of beta-barrel outer membrane proteins (OMPs), with homologous complexes found in mitochondria and chloroplasts. Despite their essential roles, exactly how these OMPs are formed remains unknown. The BAM complex consists of a central and essential component called BamA (an OMP itself) and four lipoproteins called BamB-E. While the structure of the lipoproteins have been reported, the structure of full length BamA has been elusive. Recently though, we described the structure of BamA from two species of bacteria: Neisseria gonorrhoeae and Haemophilus ducreyi. BamA consists of a large periplasmic domain attached to a 16-strand transmembrane beta-barrel domain. Together, our crystal structures and molecule dynamics (MD) simulations revealed several structural features which gave clues to the mechanism by which BamA catalyzes beta-barrel assembly. The first is that the interior cavity is accessible in one BamA structure and conformationally closed in the other. Second, an exterior rim of the beta-barrel has a distinctly narrowed hydrophobic surface, locally destabilizing the outer membrane. Third, the beta-barrel can undergo lateral opening, suggesting a route from the interior cavity in BamA into the outer membrane. And fourth, a surface exposed exit pore positioned above the lateral opening site which may play a role in the biogenesis of extracellular loops. In this presentation, the crystal structures and MD simulations of BamA will be presented along with our work looking at the role of these four structural features in the role of BamA within the BAM complex.

scholarly journals MANIFESTATION OF SOLAR ACTIVITY AND DYNAMICS OF THE ATMOSPHERE IN VARIATIONS OF 577.7 AND 630.0 nm ATMOSPHERIC EMISSIONS IN SOLAR CYCLE 24

2020 ◽  
Vol 6 (3) ◽  
pp. 81-85
Author(s):  
Aleksandr Mikhalev
Keyword(s):  
Solar Activity ◽  
Solar Cycle ◽  
Atomic Oxygen ◽  
Correlation Coefficients ◽  
Atmospheric Emissions ◽  
Annual Average ◽  
Solar Cycle 24 ◽  
Cycle 24 ◽  
Activity Indices

In the paper, variations of the night emission intensities in the 557.7 and 630 nm atomic oxygen lines [OI] in 2011–2019 have been analyzed. The analysis is based on data from the ISTP SB RAS Geophysical Observatory. The emission intensities are compared with atmospheric, solar, and geophysical parameters. High correlation coefficients between monthly average and annual average 630.0 nm emission intensities and solar activity indices F10.7 have been obtained. This suggests a key role of solar activity in variations of this emission in the period of interest. Variations of the 557.7 nm emission demonstrate to a greater extent the correlations of the stratospheric zonal wind (QBO.U30 index) with quasi-biennial oscillations. The causes of the weak dependence of the 557.7 nm emission intensity on solar activity in solar cycle 24 are discussed.

scholarly journals Moisture Induced Degradation of Porous Low-k Materials

2006 ◽  
Vol 914 ◽  
Author(s):  
Mikhail Baklanov ◽  
David O'Dwyer ◽  
Adam M Urbanowicz ◽  
Quoc Toan Le ◽  
Steven Demuynck ◽  
...  
Keyword(s):  
Water Film ◽  
Internal Surface ◽  
Hydrophobic Surface ◽  
Adsorbed Water ◽  
Bulk Water ◽  
Oh Groups ◽  
Film Bulk ◽  
Low K

AbstractInteraction of moisture with porous low-k films is evaluated by using in situ ellipsometry setup. The adsorbed water amount is calculated from change of refractive index measured during the adsorption. Pristine low-k films reversibly adsorb 2 - 5% of water that reflects presence of constitutive hydrophilic centrums. Plasma and thermal treatments increase the number of hydrophilic centrums. Once the amount of these centrums has reached a certain critical value sufficient to form a continuous water film, bulk water condensation is observed. Change of properties during the water adsorption in the damaged films is not fully reversible. Each additional adsorption cycle increases the dielectric function of the film because of decreasing porosity, increasing skeleton density and shrinkage. The pressure corresponding to the bulk condensation allows us to calculate internal contact angle (internal surface energy) of low-k materials. The water molecules adsorbed on separate OH groups play the role of a catalyst that hydrolyses the siloxane bridges initially present on hydrophobic surface.

The sorption of zinc species by clay minerals

1976 ◽  
Vol 29 (8) ◽  
pp. 1649 ◽  
Author(s):  
H Farrah ◽  
WF Pickering
Keyword(s):  
Ion Exchange ◽  
Alkaline Media ◽  
Precipitation Process ◽  
Zinc Ions ◽  
Clay Suspension ◽  
Acidic Media ◽  
Apparent Affinity ◽  
Charged Species ◽  
Nucleation Centre

The effect of changes in pH and the presence of ligands on the uptake of zinc ions by three types of clay mineral (kaolinite, illite and montmorillonite) has been investigated. In alkaline media the clay suspension acts as a nucleation centre for polymeric hydroxy species, and the major role of many ligands is to mask the precipitation process. Uncharged and negatively charged species are not sorbed to any measurable extent. In acidic media the adsorption capacity of the clays for zinc increases with pH and possible mechanisms are considered. For kaolinite and illite the controlling process appears to be the attachment of hydroxy species to particular sites on the particle edges; with montmorillonite ion exchange at negative lattice sites appears predominant. Of particular interest is the apparent affinity between montmorillonite and species containing nitrogen functional groups.

Role of atomic oxygen produced by ECR plasma in the oxidation of YBa2Cu3O7-Xthin films studied by in-situ resistivity measurement

1991 ◽  
Vol 4 (1S) ◽  
pp. S382-S384
Author(s):  
K Yamamoto ◽  
B M Liarson ◽  
C B Eom ◽  
R H Hammond ◽  
J C Bravman ◽  
...  
Keyword(s):  
Atomic Oxygen ◽  
Resistivity Measurement ◽  
Ecr Plasma

On the role of adsorbed atomic oxygen and CO2 in copper based methanol synthesis catalysts

1994 ◽  
Vol 25 (1-2) ◽  
pp. 1-10 ◽  
Author(s):  
Martin Muhler ◽  
Eric T�rnqvist ◽  
Lars P. Nielsen ◽  
Bjerne S. Clausen ◽  
Henrik Tops�e
Keyword(s):  
Methanol Synthesis ◽  
Atomic Oxygen ◽  
Synthesis Catalysts ◽  
Methanol Synthesis Catalysts

Role of the Air-Water-Solid Interface in Bacteriophage Sorption Experiments

1998 ◽  
Vol 64 (1) ◽  
pp. 304-309 ◽  
Author(s):  
Shawn S. Thompson ◽  
Markus Flury ◽  
Marylynn V. Yates ◽  
William A. Jury
Keyword(s):  
Liquid Phase ◽  
Freundlich Isotherm ◽  
Hydrophobic Surface ◽  
Water Interface ◽  
Batch Experiments ◽  
Batch Sorption ◽  
Solid Interface ◽  
Phage Adsorption ◽  
Air Water Interface

ABSTRACT Batch sorption experiments were carried out with the bacteriophages MS2 and φX174. Two types of reactor vessels, polypropylene and glass, were used. Consistently lower concentrations of MS2 were found in the liquid phase in the absence of soil (control blanks) than in the presence of soil after mixing. High levels of MS2 inactivation (∼99.9%) were observed in control tubes made of polypropylene (PP), with comparatively little loss of virus seen in PP tubes when soil was present. Minimal inactivation of MS2 was observed when the air-water interface was completely eliminated from PP control blanks during mixing. All batch experiments performed with reactor tubes made of glass demonstrated no substantial inactivation of MS2. In similar experiments, bacteriophage φX174 did not undergo inactivation in either PP or glass control blanks, implying that this virus is not affected by the same factors which led to inactivation of MS2 in the PP control tubes. When possible, phage adsorption to soil was calculated by the Freundlich isotherm. Our data suggest that forces associated with the air-water-solid interface (where the solid is a hydrophobic surface) are responsible for inactivation of MS2 in the PP control tubes. The influence of air-water interfacial forces should be carefully considered when batch sorption experiments are conducted with certain viruses.

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