Influence of the Polyelectrolyte Molecular Weight on Exponentially Growing Multilayer Films in the Linear Regime

Langmuir ◽  
2007 ◽  
Vol 23 (4) ◽  
pp. 1898-1904 ◽  
Author(s):  
C. Porcel ◽  
Ph. Lavalle ◽  
G. Decher ◽  
B. Senger ◽  
J.-C. Voegel ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Multilayer Films ◽  
Linear Regime

Effect of Molecular Weight and Arm Number on the Growth and pH-Dependent Morphology of Star Poly[2-(dimethylamino)ethyl methacrylate]/Poly(styrenesulfonate) Multilayer Films

2010 ◽  
Vol 43 (21) ◽  
pp. 9087-9093 ◽  
Author(s):  
Zhizhang Guo ◽  
Xingyu Chen ◽  
Jianyu Xin ◽  
Duo Wu ◽  
Jianshu Li ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Multilayer Films ◽  
Ethyl Methacrylate ◽  
Ph Dependent

Molecular Weight Dependence on the Disintegration of Spin-Assisted Weak Polyelectrolyte Multilayer Films

2013 ◽  
Vol 46 (11) ◽  
pp. 4580-4588 ◽  
Author(s):  
Yeongseon Jang ◽  
Jooyeon Seo ◽  
Bulent Akgun ◽  
Sushil Satija ◽  
Kookheon Char
Keyword(s):  
Molecular Weight ◽  
Multilayer Films ◽  
Polyelectrolyte Multilayer ◽  
Molecular Weight Dependence

pH-Amplified Multilayer Films Based on Hyaluronan: Influence of HA Molecular Weight and Concentration on Film Growth and Stability

2011 ◽  
Vol 12 (4) ◽  
pp. 1322-1331 ◽  
Author(s):  
Liyan Shen ◽  
Patrick Chaudouet ◽  
Jian Ji ◽  
Catherine Picart
Keyword(s):  
Molecular Weight ◽  
Film Growth ◽  
Multilayer Films

Molecular Weight Dependence of Polymer Chain Mobility within Multilayer Films

2013 ◽  
Vol 2 (10) ◽  
pp. 865-868 ◽  
Author(s):  
Li Xu ◽  
Victor Selin ◽  
Aliaksandr Zhuk ◽  
John F. Ankner ◽  
Svetlana A. Sukhishvili
Keyword(s):  
Molecular Weight ◽  
Polymer Chain ◽  
Multilayer Films ◽  
Chain Mobility ◽  
Molecular Weight Dependence

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

The External Shape of Human γ GI Immunoglobulin from Crystal Sections

1971 ◽  
Vol 29 ◽  
pp. 428-429
Author(s):  
L. W. Labaw
Keyword(s):  
Molecular Weight ◽  
Sodium Phosphate ◽  
Osmium Tetroxide ◽  
Unit Cell ◽  
Monoclinic Space Group ◽  
X Ray ◽  
Large Molecular Weight ◽  
Cell Dimensions ◽  
Unit Cell Dimensions ◽  
Crystallographic Axes

Crystals of a human γGl immunoglobulin have the external morphology of diamond shaped prisms. X-ray studies have shown them to be monoclinic, space group C2, with 2 molecules per unit cell. The unit cell dimensions are a = 194.1, b = 91.7, c = 51.6Å, 8 = 102°. The relatively large molecular weight of 151,000 and these unit cell dimensions made this a promising crystal to study in the EM.Crystals similar to those used in the x-ray studies were fixed at 5°C for three weeks in a solution of mother liquor containing 5 x 10-5M sodium phosphate, pH 7.0, and 0.03% glutaraldehyde. They were postfixed with 1% osmium tetroxide for 15 min. and embedded in Maraglas the usual way. Sections were cut perpendicular to the three crystallographic axes. Such a section cut with its plane perpendicular to the z direction is shown in Fig. 1.This projection of the crystal in the z direction shows periodicities in at least four different directions but these are only seen clearly by sighting obliquely along the micrograph.

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