pH-Amplified Multilayer Films Based on Hyaluronan: Influence of HA Molecular Weight and Concentration on Film Growth and Stability

2011 ◽  
Vol 12 (4) ◽  
pp. 1322-1331 ◽  
Author(s):  
Liyan Shen ◽  
Patrick Chaudouet ◽  
Jian Ji ◽  
Catherine Picart
2010 ◽  
Vol 43 (21) ◽  
pp. 9087-9093 ◽  
Author(s):  
Zhizhang Guo ◽  
Xingyu Chen ◽  
Jianyu Xin ◽  
Duo Wu ◽  
Jianshu Li ◽  
...  

2013 ◽  
Vol 46 (11) ◽  
pp. 4580-4588 ◽  
Author(s):  
Yeongseon Jang ◽  
Jooyeon Seo ◽  
Bulent Akgun ◽  
Sushil Satija ◽  
Kookheon Char

Langmuir ◽  
2007 ◽  
Vol 23 (4) ◽  
pp. 1898-1904 ◽  
Author(s):  
C. Porcel ◽  
Ph. Lavalle ◽  
G. Decher ◽  
B. Senger ◽  
J.-C. Voegel ◽  
...  

2013 ◽  
Vol 2 (10) ◽  
pp. 865-868 ◽  
Author(s):  
Li Xu ◽  
Victor Selin ◽  
Aliaksandr Zhuk ◽  
John F. Ankner ◽  
Svetlana A. Sukhishvili

Author(s):  
Douglas C. Barker

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.


Author(s):  
W. Bernard

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.


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