Electrochemical Impedance Analysis of Adsorption and Enzyme Kinetics of Calf Intestine Alkaline Phosphatase on SAM-Modified Gold Electrode

2012 ◽  
Vol 116 (30) ◽  
pp. 16030-16037 ◽  
Author(s):  
S. Shrikrishnan ◽  
K. Sankaran ◽  
V. Lakshminarayanan
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Kinetics ◽  
Gold Electrode ◽  
Electrochemical Impedance ◽  
Impedance Analysis ◽  
Calf Intestine Alkaline Phosphatase ◽  
Intestine Alkaline Phosphatase ◽  
Kinetics Of

scholarly journals Device for quantitative cytochemistry: a computerized scanning microdensitometer 'grain counter'.

1978 ◽  
Vol 26 (10) ◽  
pp. 792-802 ◽  
Author(s):  
N S Markovic ◽  
L E Lipkin ◽  
O S Markovic ◽  
M J Wade
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Enzyme Kinetics ◽  
Single Cell ◽  
Quantitative Data ◽  
Single Cells ◽  
Final Reaction Product ◽  
Chamber Studies ◽  
Kinetics Of ◽  
Quantitative Cytochemistry

A computerized scanning microdensitometer and autoradiographic grain counter was able to provide quantitative data on the cytochemical final reaction product formed within a single cell and also quantitate the kinetics of its formation. Optical density and area measurements were performed on hundreds of leukocytes from slides previously stained to demonstrate any one of a variety of reactions. These included cellular glycogen, lipids, peroxidase, esterases, alkaline phosphatase, and acid phosphatase. In addition to these slide studies, chamber studies with an adapted Dvorak-Stotler Chamber allowed the measurement of enzyme kinetics within single cells.

Calix[4]arene methylenebisphosphonic acids as calf intestine alkaline phosphatase inhibitors

2004 ◽  
Vol 2 (21) ◽  
pp. 3162 ◽  
Author(s):  
Andriy I. Vovk ◽  
Vitaly I. Kalchenko ◽  
Sergey A. Cherenok ◽  
Valery P. Kukhar ◽  
Oxana V. Muzychka ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Inhibitors ◽  
Calf Intestine Alkaline Phosphatase ◽  
Intestine Alkaline Phosphatase

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1968 ◽  
Vol 19 (03/04) ◽  
pp. 364-367 ◽  
Author(s):  
H. C Hemker ◽  
P. W Hemker
Keyword(s):  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Competitive Inhibition ◽  
Competitive Inhibitor ◽  
Kinetics Of

SummaryThe enzyme kinetics of competitive inhibition under conditions prevailing in clotting tests are developed and a method is given to measure relative amounts of a competitive inhibitor by means of the t — D plot.

Characterizations of Synthetic 8mol% YSZ with Comparison to 3mol %YSZ for HT-SOFC

2020 ◽  
Vol 38 (4A) ◽  
pp. 491-500
Author(s):  
Abeer F. Al-Attar ◽  
Saad B. H. Farid ◽  
Fadhil A. Hashim
Keyword(s):  
Ionic Conductivity ◽  
Electrochemical Impedance ◽  
Structural Information ◽  
Impedance Analysis ◽  
High Energy ◽  
Yttria Stabilized Zirconia ◽  
X Ray Diffraction ◽  
Stabilized Zirconia ◽  
Powder Morphology ◽  
X Ray

In this work, Yttria (Y2O3) was successfully doped into tetragonal 3mol% yttria stabilized Zirconia (3YSZ) by high energy-mechanical milling to synthesize 8mol% yttria stabilized Zirconia (8YSZ) used as an electrolyte for high temperature solid oxide fuel cells (HT-SOFC). This work aims to evaluate the densification and ionic conductivity of the sintered electrolytes at 1650°C. The bulk density was measured according to ASTM C373-17. The powder morphology and the microstructure of the sintered electrolytes were analyzed via Field Emission Scanning Electron Microscopy (FESEM). The chemical analysis was obtained with Energy-dispersive X-ray spectroscopy (EDS). Also, X-ray diffraction (XRD) was used to obtain structural information of the starting materials and the sintered electrolytes. The ionic conductivity was obtained through electrochemical impedance spectroscopy (EIS) in the air as a function of temperatures at a frequency range of 100(mHz)-100(kHz). It is found that the 3YSZ has a higher density than the 8YSZ. The impedance analysis showed that the ionic conductivity of the prepared 8YSZ at 800°C is0.906 (S.cm) and it was 0.214(S.cm) of the 3YSZ. Besides, 8YSZ has a lower activation energy 0.774(eV) than that of the 3YSZ 0.901(eV). Thus, the prepared 8YSZ can be nominated as an electrolyte for the HT-SOFC.

Electrochemical Impedance Analysis of the Wear of Planographic Plates

CORROSION ◽  
1991 ◽  
Vol 47 (1) ◽  
pp. 67-73 ◽  
Author(s):  
M. W. Kendig ◽  
S. Breitweiser ◽  
E. Hudyma
Keyword(s):  
Electrochemical Impedance ◽  
Impedance Analysis

scholarly journals Fundamental Mechanisms of Copper CMP – Passivation Kinetics of Copper in CMP Slurry Constituents

2009 ◽  
Vol 1157 ◽  
Author(s):  
Shantanu Tripathi ◽  
Fiona M. Doyle ◽  
David A. Dornfeld
Keyword(s):  
Electrochemical Impedance ◽  
Protective Film ◽  
Current Decay ◽  
Glycine Solution ◽  
Current Densities ◽  
Circuit Elements ◽  
Copper Cmp ◽  
Cu Cmp ◽  
Kinetics Of ◽  
Capacitive Charging

AbstractDuring copper CMP, abrasives and asperities interact with the copper at the nano-scale, partially removing protective films. The local Cu oxidation rate increases, then decays with time as the protective film reforms. In order to estimate the copper removal rate and other Cu-CMP output parameters with a mechanistic model, the passivation kinetics of Cu, i.e. the decay of the oxidation current with time after an abrasive/copper interaction, are needed. For the first time in studying Cu-CMP, microelectrodes were used to reduce interference from capacitive charging, IR drops and low diffusion limited currents, problems typical with traditional macroelectrodes. Electrochemical impedance spectroscopy (EIS) was used to obtain the equivalent circuit elements associated with different electrochemical phenomena (capacitive, kinetics, diffusion etc.) at different polarization potentials. These circuit elements were used to interpret potential-step chronoamperometry results in inhibiting and passivating solutions, notably to distinguish between capacitive charging and Faradaic currents.Chronoamperometry of Cu in acidic aqueous glycine solution containing the corrosion inhibitor benzotriazole (BTA) displayed a very consistent current decay behavior at all potentials, indicating that the rate of current decay was controlled by diffusion of BTA to the surface. In basic aqueous glycine solution, Cu (which undergoes passivation by a mechanism similar to that operating in weakly acidic hydrogen peroxide slurries) displayed similar chronoamperometric behavior for the first second or so at all anodic potentials. Thereafter, the current densities at active potentials settled to values around those expected from polarization curves, whereas the current densities at passive potentials continued to decline. Oxidized Cu species typically formed at ‘active’ potentials were found to cause significant current decay at active potentials and at passive potentials before more protective passive films form. This was established from galvanostatic experiments.

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