Isothermal Titration Calorimetric and Electromotive Force Studies on Binding Interactions of Hydrophobic Ethoxylated Urethane and Sodium Dodecyl Sulfate of Different Molecular Masses

2004 ◽  
Vol 108 (16) ◽  
pp. 4979-4988 ◽  
Author(s):  
S. Dai ◽  
K. C. Tam ◽  
E. Wyn-Jones ◽  
R. D. Jenkins
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Electromotive Force ◽  
Sodium Dodecyl ◽  
Binding Interactions ◽  
Dodecyl Sulfate ◽  
Molecular Masses

Ubiquitin immunoreactivity shows several proteins varying with development and sporulation in the basidiomycete Coprinus cinereus

1990 ◽  
Vol 68 (7-8) ◽  
pp. 1019-1025 ◽  
Author(s):  
Tosaku Kanda ◽  
Nobuharu Tanaka ◽  
Tsuneo Takemaru
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Light Period ◽  
Coprinus Cinereus ◽  
Polyacrylamide Gels ◽  
Wild Type ◽  
Stages Of Development ◽  
Crude Extracts ◽  
Dodecyl Sulfate ◽  
Molecular Masses

Crude extracts of mycelia and basidiocarp primordia in the basidiomycete Coprinus cinereus were resolved on sodium dodecyl sulfate – polyacrylamide gels, and ubiquitin and several proteins were detected by immunoblotting with anti-ubiquitin antibody. The molecular masses of the proteins detected were 30 900, 28 600, 27 800, 26 300, 22 500, and 15 400 daltons, respectively. Relative levels of ubiquitin and the ubiquitin-immunoreactive proteins were measured in different stages of development. The levels of ubiquitin and most of the ubiquitin-immunoreactive proteins in basidiocarp primordium formation increased and in basidiocarp maturation decreased in cap and upper stipe, while in lower stipe became high except for the 27 800 dalton protein and ubiquitin. During sporulation, ubiquitin and all the ubiquitin-immunoreactive proteins tended to decrease in the cap of the young wild-type basidiocarp. The levels of 30 900 and 15 400 dalton proteins increased transiently at 6–10 h after the beginning of the last light period, while ubiquitin decreased markedly. No correlation was observed between changes in levels of the ubiquitin-immunoreactive proteins and the blocked stages in sporulation-deficient mutants.Key words: ubiquitin, development, sporulation, Coprinus.

Electrophoretic characterization of endo-(1,4)-β-glucanases secreted during assimilative growth and antheridiol-induced branching inAchlya ambisexualis

1996 ◽  
Vol 42 (6) ◽  
pp. 557-561 ◽  
Author(s):  
Terry W. Hill
Keyword(s):  
Enzyme Activity ◽  
Sodium Dodecyl Sulfate ◽  
Cell Walls ◽  
Sodium Dodecyl ◽  
Achlya Ambisexualis ◽  
Dodecyl Sulfate ◽  
Exclusion Chromatography ◽  
Molecular Masses

Secreted endo-(1,4)-β-glucanases ("cellulases") of Achlya ambisexualis were analyzed by a technique that permits visualization of enzyme activity in situ after electrophoresis in gels containing sodium dodecyl sulfate. Catalytic polypeptides with molecular masses of about 97, 74, 36, 29, and 25 kDa were observed in media from young cultures, though progressively fewer bands were observed as cultures aged. Based on size estimations of native enzymes with gel exclusion chromatography, the 97- and 36-kDa polypeptides were concluded to be subunits of a 245-kDa holoenzyme and the 25-kDa polypeptides were concluded to be subunits of a second holoenzyme of about 92 kDa. The data were insufficient to allow similar assignments for the more ephemeral 74- and 29-kDa polypeptides. The endoglucanases secreted during branch induction by antheridiol or 0.2% peptone comigrated in electrophoretic gels with enzymes secreted during normal assimilative growth. No endoglucanases specific to induced branching were observed.Key words: oomycetes, cell walls, endoglucanases, cellulases, antheridiol.

scholarly journals Partial Purification of Integral Membrane Antigenic Proteins fromTrypanosoma evansiThat Display Immunological Cross-Reactivity withTrypanosoma vivax

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Norma P. Velásquez ◽  
Rocío E. Camargo ◽  
Graciela L. Uzcanga ◽  
José Bubis
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Cross Reactivity ◽  
Trypanosoma Evansi ◽  
Partial Purification ◽  
Limiting Factor ◽  
Dodecyl Sulfate ◽  
Causative Agents ◽  
Molecular Masses ◽  
Triton X

Trypanosoma evansiandTrypanosoma vivax, which are the major causative agents of animal trypanosomosis in Venezuela, have shown a very high immunological cross-reactivity. Since the production ofT. vivaxantigens is a limiting factor as this parasite is difficult to propagate in experimental animal models, our goal has been to identify and isolate antigens fromT. evansithat cross-react withT. vivax. Here, we used the VenezuelanT. evansiTEVA1 isolate to prepare the total parasite lysate and its corresponding cytosolic and membranous fractions. In order to extract theT. evansiintegral membrane proteins, the particulate portion was further extracted first with Triton X-100, and then with sodium dodecyl sulfate. After discarding the cytosolic and Triton X-100 solubilized proteins, we employed sedimentation by centrifugation on linear sucrose gradients to partially purify the sodium dodecyl sulfate-solubilized proteins from the Triton X-100 resistant particulate fraction ofT. evansi. We obtained enriched pools containing polypeptide bands with apparent molecular masses of 27 kDa, 31 kDa, and 53 kDa, which were recognized by anti-T. vivaxantibodies from experimentally and naturally infected bovines.

Electromotive Force Studies Associated with the Binding of Sodium Dodecyl Sulfate to a Range of Nonionic Polymers

Langmuir ◽  
1999 ◽  
Vol 15 (13) ◽  
pp. 4380-4387 ◽  
Author(s):  
S. M. Ghoreishi ◽  
Y. Li ◽  
D. M. Bloor ◽  
J. Warr ◽  
E. Wyn-Jones
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Electromotive Force ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate ◽  
Nonionic Polymers

Binding of Sodium Dodecyl Sulfate to Some Polyethyleneimines and Their Ethoxylated Derivatives at Different pH Values. Electromotive Force and Microcalorimetry Studies

Langmuir ◽  
2000 ◽  
Vol 16 (7) ◽  
pp. 3093-3100 ◽  
Author(s):  
Y. Li ◽  
S. M. Ghoreishi ◽  
J. Warr ◽  
D. M. Bloor ◽  
J. F. Holzwarth ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Electromotive Force ◽  
Sodium Dodecyl ◽  
Ph Values ◽  
Dodecyl Sulfate
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