Electrophoretic characterization of endo-(1,4)-β-glucanases secreted during assimilative growth and antheridiol-induced branching inAchlya ambisexualis

1996 ◽  
Vol 42 (6) ◽  
pp. 557-561 ◽  
Author(s):  
Terry W. Hill
Keyword(s):  
Enzyme Activity ◽  
Sodium Dodecyl Sulfate ◽  
Cell Walls ◽  
Sodium Dodecyl ◽  
Achlya Ambisexualis ◽  
Dodecyl Sulfate ◽  
Exclusion Chromatography ◽  
Molecular Masses

Secreted endo-(1,4)-β-glucanases ("cellulases") of Achlya ambisexualis were analyzed by a technique that permits visualization of enzyme activity in situ after electrophoresis in gels containing sodium dodecyl sulfate. Catalytic polypeptides with molecular masses of about 97, 74, 36, 29, and 25 kDa were observed in media from young cultures, though progressively fewer bands were observed as cultures aged. Based on size estimations of native enzymes with gel exclusion chromatography, the 97- and 36-kDa polypeptides were concluded to be subunits of a 245-kDa holoenzyme and the 25-kDa polypeptides were concluded to be subunits of a second holoenzyme of about 92 kDa. The data were insufficient to allow similar assignments for the more ephemeral 74- and 29-kDa polypeptides. The endoglucanases secreted during branch induction by antheridiol or 0.2% peptone comigrated in electrophoretic gels with enzymes secreted during normal assimilative growth. No endoglucanases specific to induced branching were observed.Key words: oomycetes, cell walls, endoglucanases, cellulases, antheridiol.

Preparation and Characterization of Polypyrrole-Coated Silicon Nanoparticles

2007 ◽  
Vol 119 ◽  
pp. 295-298
Author(s):  
Hyun Shil La ◽  
Joon Pyo Jeun ◽  
Jae Hak Choi ◽  
Phil Hyun Kang ◽  
Youn Sik Lee ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Silicon Nanoparticles ◽  
In Situ Polymerization ◽  
Sodium Dodecyl ◽  
Average Thickness ◽  
Si Nanoparticles ◽  
Dodecyl Sulfate ◽  
Discharge Capacities

Silicon (Si) nanoparticles were stabilized by sodium dodecyl sulfate and poly(Nvinylpyrrolidone) in water, and coated with polypyrrole (PPy) via an in-situ polymerization of pyrrole with FeCl3. TEM images revealed that the Si nanoparticles were successfully coated with PPy (average thickness, ~2 nm). The Li/PPy-coated Si electrode exhibited improved discharge capacities, when compared to that of a reported Li/pure Si electrode. PPy-coatings on the Si nanoparticles acted as efficient conducting agents and prevented an extraction of the cracked Si fragments into the electrolyte to some extent.

Ubiquitin immunoreactivity shows several proteins varying with development and sporulation in the basidiomycete Coprinus cinereus

1990 ◽  
Vol 68 (7-8) ◽  
pp. 1019-1025 ◽  
Author(s):  
Tosaku Kanda ◽  
Nobuharu Tanaka ◽  
Tsuneo Takemaru
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Light Period ◽  
Coprinus Cinereus ◽  
Polyacrylamide Gels ◽  
Wild Type ◽  
Stages Of Development ◽  
Crude Extracts ◽  
Dodecyl Sulfate ◽  
Molecular Masses

Crude extracts of mycelia and basidiocarp primordia in the basidiomycete Coprinus cinereus were resolved on sodium dodecyl sulfate – polyacrylamide gels, and ubiquitin and several proteins were detected by immunoblotting with anti-ubiquitin antibody. The molecular masses of the proteins detected were 30 900, 28 600, 27 800, 26 300, 22 500, and 15 400 daltons, respectively. Relative levels of ubiquitin and the ubiquitin-immunoreactive proteins were measured in different stages of development. The levels of ubiquitin and most of the ubiquitin-immunoreactive proteins in basidiocarp primordium formation increased and in basidiocarp maturation decreased in cap and upper stipe, while in lower stipe became high except for the 27 800 dalton protein and ubiquitin. During sporulation, ubiquitin and all the ubiquitin-immunoreactive proteins tended to decrease in the cap of the young wild-type basidiocarp. The levels of 30 900 and 15 400 dalton proteins increased transiently at 6–10 h after the beginning of the last light period, while ubiquitin decreased markedly. No correlation was observed between changes in levels of the ubiquitin-immunoreactive proteins and the blocked stages in sporulation-deficient mutants.Key words: ubiquitin, development, sporulation, Coprinus.

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