Enzymic synthesis of L-acetylcarnitine and citric acid using acetyl coenzyme A recycling

1986 ◽  
Vol 51 (14) ◽  
pp. 2842-2844 ◽  
Author(s):  
Smita S. Patel ◽  
Hugh D. Conlon ◽  
David R. Walt
Keyword(s):  
Citric Acid ◽  
Coenzyme A ◽  
Acetyl Coenzyme A ◽  
Enzymic Synthesis ◽  
Acetyl Coenzyme

THE ENZYMATIC SYNTHESIS OF CITRIC ACID BY CELL-FREE EXTRACTS OF ASPERGILLUS NIGER

1954 ◽  
Vol 32 (4) ◽  
pp. 434-439 ◽  
Author(s):  
C. V. Ramakrishnan ◽  
S. M. Martin
Keyword(s):  
Citric Acid ◽  
Calcium Phosphate ◽  
Aspergillus Niger ◽  
Ammonium Sulphate ◽  
Enzymatic Synthesis ◽  
Coenzyme A ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme ◽  
Ammonium Sulphate Fractionation ◽  
Condensing Enzyme

Cell-free extracts of Aspergillus niger, N.R.C. 233, have been shown to contain the enzymes necessary to catalyze the synthesis of citrate from ATP, acetate, and oxalacetate. The "condensing enzyme", which catalyzes the condensation of acetyl-coenzyme A and oxalacetate to yield citrate, has been isolated and purified approximately 50-fold by a combination of steps involving ammonium sulphate fractionation and calcium phosphate gel adsorption.

THE ENZYMATIC SYNTHESIS OF CITRIC ACID BY CELL-FREE EXTRACTS OF ASPERGILLUS NIGER

1954 ◽  
Vol 32 (1) ◽  
pp. 434-439 ◽  
Author(s):  
C. V. Ramakrishnan ◽  
S. M. Martin
Keyword(s):  
Citric Acid ◽  
Calcium Phosphate ◽  
Aspergillus Niger ◽  
Ammonium Sulphate ◽  
Enzymatic Synthesis ◽  
Coenzyme A ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme ◽  
Ammonium Sulphate Fractionation ◽  
Condensing Enzyme

Cell-free extracts of Aspergillus niger, N.R.C. 233, have been shown to contain the enzymes necessary to catalyze the synthesis of citrate from ATP, acetate, and oxalacetate. The "condensing enzyme", which catalyzes the condensation of acetyl-coenzyme A and oxalacetate to yield citrate, has been isolated and purified approximately 50-fold by a combination of steps involving ammonium sulphate fractionation and calcium phosphate gel adsorption.

Enzyme-catalyzed synthesis of citric acid using acetyl-coenzyme a recycling in a two-phase system

1990 ◽  
Vol 18 (2) ◽  
pp. 131-135 ◽  
Author(s):  
Tianmei Ouyang ◽  
David R. Walt ◽  
Smita S. Patel
Keyword(s):  
Citric Acid ◽  
Coenzyme A ◽  
Phase System ◽  
Two Phase ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme
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