scholarly journals Comparison of Fumonisin Contamination Using HPLC and ELISA Methods inBtand Near-Isogenic Maize Hybrids Infested with European Corn Borer or Western Bean Cutworm

2014 ◽  
Vol 62 (27) ◽  
pp. 6463-6472 ◽  
Author(s):  
Erin Bowers ◽  
Richard Hellmich ◽  
Gary Munkvold
Keyword(s):  
European Corn Borer ◽  
Corn Borer ◽  
Maize Hybrids ◽  
Western Bean Cutworm

LODGING-ENHANCING TECHNIQUES FOR USE ON CORN PERFORMANCE TRIALS IN SHORT-SEASON AREAS

1982 ◽  
Vol 62 (2) ◽  
pp. 299-304
Author(s):  
S. TWUMASI-AFRIYIE ◽  
R. B. HUNTER
Keyword(s):  
European Corn Borer ◽  
Zea Mays L ◽  
Cultural Practices ◽  
Gibberella Zeae ◽  
Lodging Resistance ◽  
Corn Borer ◽  
Maize Hybrids ◽  
Stalk Rot ◽  
Wide Range ◽  
Stalk Lodging

A study was conducted during the 2 yr, 1978 and 1979, to determine an effective method of enhancing stalk lodging in maize (Zea mays L.) with the objective of being able to differentiate among hybrids for lodging susceptibility under conditions of low natural lodging. Six maize hybrids representing a wide range of stalk lodging resistance were used in the study. The techniques utilized were inoculating plants with a stalk rot pathogen (Gibberella zeae (Schw.) Petch) and/or infesting plants with European corn borer (Ostrinia nubilalis Hbn.). Both techniques, individually or in combination, were effective in enhancing lodging. Under environmental conditions and cultural practices where natural stalk lodging was low and differences in stalk lodging among hybrids would have otherwise been masked, the two lodging-enhancing techniques allowed for improved detection of hybrid differences. Both treatment effects and differences among hybrids for stalk lodging increased as stalk lodging rating was delayed, indicating delayed rating is preferable. Disadvantages to delayed rating are discussed.

scholarly journals The impact of Bacillus thuringiensis technology on the occurrence of fumonisins and other mycotoxins in maize

2016 ◽  
Vol 9 (3) ◽  
pp. 475-486 ◽  
Author(s):  
J. Díaz-Gómez ◽  
S. Marín ◽  
T. Capell ◽  
V. Sanchis ◽  
A.J. Ramos
Keyword(s):  
Bacillus Thuringiensis ◽  
Fungal Infection ◽  
European Corn Borer ◽  
Insect Pests ◽  
Animal Feed ◽  
Bt Maize ◽  
Cry1ab Protein ◽  
Corn Borer ◽  
Maize Hybrids ◽  
The Impact

In many developing countries, maize is both a staple food crop and a widely-used animal feed. However, adventitious colonisation or damage caused by insect pests allows fungi to penetrate the vegetative parts of the plant and the kernels, the latter resulting in mycotoxin contamination. Maize seeds contaminated with fumonisins and other mycotoxins pose a serious threat to both humans and livestock. However, numerous studies have reported a significant reduction in pest damage, disease symptoms and fumonisin levels in maize hybrids expressing the Bacillus thuringiensis (Bt) gene cry1Ab, particularly in areas where the European corn borer is prevalent. When other pests are also present, the cry1Ab gene alone offers insufficient protection, and combinations of insecticidal genes are required to reduce damage to plants caused by insects. The combination of Cry1Ab protein with other Cry proteins (such as Cry1F) or Vip proteins has reduced the incidence of pests and, indirectly, mycotoxin levels. Maize hybrids expressing multiple Bt genes, such as SmartStax®, are less susceptible to damage by insects, but mycotoxin levels are not routinely and consistently compared in these crops. Bt maize has a greater economic impact on Fusarium toxins than aflatoxins. The main factors that determine the effectiveness of Bt hybrids are the type of pest and the environmental conditions, but the different fungal infection pathways must also be considered. An alternative strategy to reduce mycotoxin levels in crops is the development of transgenic plants expressing genes that protect against fungal infection or reduce mycotoxin levels by in situ detoxification. In this review article, we summarise what is known about the relationship between the cultivation of Bt maize hybrids and contamination levels with different types of mycotoxins.

Vip3Aa and Cry1Ab proteins in maize reduce Fusarium ear rot and fumonisins by deterring kernel injury from multiple Lepidopteran pests

2013 ◽  
Vol 6 (2) ◽  
pp. 127-135 ◽  
Author(s):  
E. Bowers ◽  
R. Hellmich ◽  
G. Munkvold
Keyword(s):  
Insect Resistance ◽  
European Corn Borer ◽  
Grain Quality ◽  
Block Design ◽  
Corn Earworm ◽  
Fusarium Ear Rot ◽  
Ear Rot ◽  
Corn Borer ◽  
Maize Hybrids ◽  
Quality Measurements

Field trials were conducted in 2008, 2009 and 2011 to assess fumonisin contamination in transgenic (Bt) and non- Bt maize hybrids infested with European corn borer, corn earworm, and Western bean cutworm. Comparisons were made among maize hybrids expressing two transgenic insect resistance proteins (Cry1Ab × Vip3Aa), a single resistance protein (Cry1Ab), or no insect resistance. The field design was a randomised complete block design with four replicates of each hybrid × insect combination. Kernel injury, Fusarium ear rot, and fumonisins (FB1+FB2+FB3) in maize grain were measured. These measurements differed significantly among years of the study. In all years, significant positive correlations were present between insect injury and Fusarium ear rot, insect injury and grain fumonisin levels, and Fusarium ear rot and grain fumonisin levels. Under all insect infestation treatments, Cry1Ab × Vip3Aa hybrids were the most resistant of the hybrids with regard to any of the grain quality measurements. Averaged over all insect infestations and years, insect injury, Fusarium ear rot, and grain fumonisin levels were all low in Cry1Ab × Vip3Aa (0.1% and 2.2% of total kernels, and 0.56 mg/kg, respectively). The highest average levels of insect injury, Fusarium ear rot, and grain fumonisin contamination (3.3% and 7.2% of total kernels, and 5.47 mg/kg, respectively) were found in the non-Bt hybrids. The presence of transgenic insect protection (Cry1Ab × Vip3Aa or Cry1Ab) resulted in significant reductions in all grain quality measurements as compared with the non- Bt hybrids. Only grain obtained from Cry1Ab × Vip3Aa hybrids consistently had acceptable fumonisin content according to both US guidance levels and EU regulatory limits. These results indicate that Cry1Ab × Vip3Aa maize hybrids are more likely to yield high quality, low-fumonisin grain compared to hybrids expressing only Cry1Ab or lacking insect resistance.

scholarly journals The effect of insecticides on the total percentage of Ostrinia nubilalis Hbn attack on maize hybrids

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 351-365
Author(s):  
Snezana Gosic-Dondo ◽  
Dragan Grcak ◽  
Milosav Grcak ◽  
Danijela Kondic ◽  
Djuradj Hajder ◽  
...  
Keyword(s):  
Ostrinia Nubilalis ◽  
European Corn Borer ◽  
First Generation ◽  
Plant Protection ◽  
Control Group ◽  
Corn Borer ◽  
Protection Effect ◽  
Maize Hybrids ◽  
Total Percentage ◽  
Active Substances

The objective of the paper was to determine the effect of insecticides on the development of the European corn borer (Ostinia nubilalis Hbn) and the effect of damage in six maize hybrids of different maturity groups. The research involved the following hybrids: ZP 427, ZP 434, ZP 555, ZP 600, ZP 606 and ZP 666. In the field trial, insecticides were applied in two terms, during the flight of O. nubilalis first generation and during the flight of second O. nubilalis generation. We used Fobos EC insecticide and a combination of Match 050 EC + Nurelle D insecticides, whose active substances are different. The active substance in Fobos EC insecticide is bifenthrin 100 g l-1, in Match 050 EC lufenuron 50 g l-1 and, in Nurelle D insecticide, the active ingredient is a combination of chlorpyrifos 500 g l-1 and cypermethrin 50 g l-1. The damage inflicted on plants was assessed in July and September. Fobos insecticide had the best plant protection effect, after its use, the arithmetic mean of the total attack percentage of all hybrids was approximately 81.62% while in the control group, it was approximately 91.72%.

scholarly journals Comparison of Bt (Bacillus thuringiensis Berliner) Maize and Conventional Measures for Control of the European Corn Borer (Lepidoptera: Crambidae)

2000 ◽  
Vol 35 (2) ◽  
pp. 118-128 ◽  
Author(s):  
Thomas L. Clark ◽  
J. E. Foster ◽  
Shripat T. Kamble ◽  
E. A. Heinrichs
Keyword(s):  
Bacillus Thuringiensis ◽  
Ostrinia Nubilalis ◽  
European Corn Borer ◽  
Field Experiments ◽  
Control Measures ◽  
Bt Maize ◽  
Corn Borer ◽  
Maize Hybrids ◽  
Grain Yields ◽  
Plant Length

Field experiments were conducted in 1997 to compare the efficacy Bt (Bacillus thuringiensis Berliner) maize hybrids and two conventional measures for control of the European corn borer, Ostrinia nubilalis (Hübner). Treatments consisted of transgenic Bt maize hybrids and their non-Bt isolines, and isolines treated with a formulated Bt or permethrin insecticide. All control measures significantly reduced O. nubilalis damage in terms of tunnels per plant, length of tunneling, and larvae per plant. The following hierarchy in terms of O. nubilalis efficacy was observed: transgenic Bt > permethrin > formulated Bt > control. In most cases, transgenic Bt maize was most effective in preventing European corn borer damage to ear shanks and generally produced the highest grain yields.

scholarly journals Fungal Species Composition in Maize Stalks in Relation to European Corn Borer Injury and Transgenic Insect Protection

Plant Disease ◽  
2002 ◽  
Vol 86 (10) ◽  
pp. 1156-1162 ◽  
Author(s):  
E. W. Gatch ◽  
G. P. Munkvold
Keyword(s):  
Species Composition ◽  
European Corn Borer ◽  
Field Experiments ◽  
Fungal Species ◽  
Gibberella Zeae ◽  
Colletotrichum Graminicola ◽  
Corn Borer ◽  
Maize Hybrids ◽  
Stalk Rot ◽  
The Mean

The maize stalk rot complex is comprised of several fungal pathogens, including Gibberella zeae, Colletotrichum graminicola, Stenocarpella maydis, and several members of the genus Fusarium. The European corn borer (ECB) (Ostrinia nubilalis) can contribute to stalk rot development by creating entry wounds and by serving as a vector of some stalk rot pathogens, particularly Fusarium verticillioides. Transgenic insect protection of maize hybrids with insecticidal proteins derived from Bacillus thuringiensis greatly reduces ECB injury and may therefore alter the species composition and diversity of the stalk rot complex. Field experiments were conducted in 1998, 1999, and 2000 to compare the species composition and diversity of fungi infecting stalks of Bt and non-Bt maize hybrids. Hybrids representing five Bt types (or “events”) and their near-isogenic non-Bt counterparts were subjected to manual and natural infestations with ECB larvae. Stalk tissue samples were cultured to determine fungal species composition. At least one species was isolated from nearly every stalk and from both diseased and symptomless tissues. G. zeae was the most common species in 1998 and 1999, but C. graminicola was most common in 2000. The mean proportions of stalks infected with F. verticillioides, F. proliferatum, and F. subglutinans were significantly lower in Bt hybrids than in non-Bt hybrids in 2 of the 3 years. Conversely, the mean proportion of stalks infected with G. zeae was higher in some Bt hybrids than their non-Bt counterparts in two of the three years. F. verticillioides was more likely to be isolated from ECB-injured tissue, whereas G. zeae and C. graminicola were more likely to be isolated from tissue not associated with ECB injury. The overall species diversity of the stalk rot complex was lower in some Bt hybrids compared with their non-Bt counterparts in 1998 and 1999. ECB activity appeared to alter fungal species composition in stalks, reflecting the association between ECB injury and specific stalk rot pathogens, particularly F. verticillioides. The species composition of fungi infecting stalks of Bt hybrids differed from that of non-Bt hybrids, but the implications of this result are not yet clear.

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