Release of Small Phenolic Compounds from Brewer’s Spent Grain and Its Lignin Fractions by Human Intestinal Microbiota in Vitro

2013 ◽  
Vol 61 (40) ◽  
pp. 9744-9753 ◽  
Author(s):  
Anna-Marja Aura ◽  
Piritta Niemi ◽  
Ismo Mattila ◽  
Klaus Niemelä ◽  
Annika Smeds ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Intestinal Microbiota ◽  
Brewer’S Spent Grain ◽  
Human Intestinal Microbiota ◽  
Spent Grain

scholarly journals Bioactive compounds from brewer’s spent grain: phenolic compounds, fatty acids and in vitro antioxidant capacity

2017 ◽  
Vol 39 (3) ◽  
pp. 269 ◽  
Author(s):  
Aline Da Rosa Almeida ◽  
Márcia Regina Ferreira Geraldo ◽  
Leomara Floriano Ribeiro ◽  
Marcos Vieira Silva ◽  
Matheus Vinicius De Oliveira Brisola Maciel ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Polyunsaturated Fatty Acids ◽  
Bioactive Compounds ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Brewer’S Spent Grain ◽  
Spent Grain

 Brewer's spent grain (BSG) was characterized by physicochemical, total phenolic compound and flavonoids contents. Antioxidant activity was evaluated by four different assays. The chromatographic analyses were used to quantify the phenolic compounds and the fatty acids in BSG. Ethanolic extracts were tested to evaluate antibacterial activity. The higher concentration of total phenolic compounds for BSG was obtained in the extraction with ethanol 20%. BSG showed an antioxidant potential for all tests evaluated. In the case of chromatographic analysis, phenolic acids and flavonoids, such as syringic acid and catechin, respectively, were detected in high quantities. Regarding to the fatty acids profile, polyunsaturated fatty acids, such as linoleic and oleic acids, were found in significant amounts. No antibacterial activity was reported for bacterial cultures and concentrations tested. BSG may be considered a protein source, rich in fiber, polyunsaturated fatty acids and bioactive compounds with antioxidant potential. 

scholarly journals Impact of Chronic Tetracycline Exposure on Human Intestinal Microbiota in a Continuous Flow Bioreactor Model

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 886
Author(s):  
Youngbeom Ahn ◽  
Ji Young Jung ◽  
Ohgew Kweon ◽  
Brian T. Veach ◽  
Sangeeta Khare ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Intestinal Microbiota ◽  
Continuous Flow ◽  
Antimicrobial Drug ◽  
Bioreactor Culture ◽  
Analysis Techniques ◽  
Human Intestinal Microbiota ◽  
Traditional Cultures ◽  
The Impact

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.

Effect of solid-state fermentation over the release of phenolic compounds from brewer's spent grain revealed by UPLC-MSE

LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 110136 ◽  
Author(s):  
Ingrid da Costa Maia ◽  
Carolina Thomaz dos Santos D'Almeida ◽  
Denise Maria Guimarães Freire ◽  
Elisa d'Avila Costa Cavalcanti ◽  
Luiz Claudio Cameron ◽  
...  
Keyword(s):  
Solid State ◽  
Phenolic Compounds ◽  
Solid State Fermentation ◽  
Brewer’S Spent Grain ◽  
Spent Grain

Interactions of a Lignin-Rich Fraction from Brewer’s Spent Grain with Gut Microbiota in Vitro

2013 ◽  
Vol 61 (27) ◽  
pp. 6754-6762 ◽  
Author(s):  
Piritta Niemi ◽  
Anna-Marja Aura ◽  
Johanna Maukonen ◽  
Annika I. Smeds ◽  
Ismo Mattila ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Brewer’S Spent Grain ◽  
Spent Grain

Fermentation in vitro of EGCG, GCG and EGCG3"Me isolated from Oolong tea by human intestinal microbiota

2013 ◽  
Vol 54 (2) ◽  
pp. 1589-1595 ◽  
Author(s):  
Xin Zhang ◽  
Xiuling Zhu ◽  
Yongkang Sun ◽  
Bing Hu ◽  
Yi Sun ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Oolong Tea ◽  
Human Intestinal Microbiota

In vitro extraction and fermentation of polyphenols from grape seeds (Vitis vinifera) by human intestinal microbiota

2016 ◽  
Vol 7 (4) ◽  
pp. 1959-1967 ◽  
Author(s):  
Li Zhou ◽  
Wei Wang ◽  
Jun Huang ◽  
Yu Ding ◽  
Zhouqiang Pan ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Intestinal Microbiota ◽  
Extraction Yield ◽  
Liquid Extraction ◽  
Pressurized Liquid Extraction ◽  
Grape Seeds ◽  
Total Polyphenols ◽  
Human Intestinal Microbiota

The effects of several parameters on the extraction yield of total polyphenols from grape seeds by pressurized liquid extraction were investigated.

scholarly journals In Vitro Bioconversion of Polyphenols from Black Tea and Red Wine/Grape Juice by Human Intestinal Microbiota Displays Strong Interindividual Variability

2010 ◽  
Vol 58 (18) ◽  
pp. 10236-10246 ◽  
Author(s):  
Gabriele Gross ◽  
Doris M. Jacobs ◽  
Sonja Peters ◽  
Sam Possemiers ◽  
John van Duynhoven ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Red Wine ◽  
Interindividual Variability ◽  
Grape Juice ◽  
Black Tea ◽  
Wine Grape ◽  
Human Intestinal Microbiota

scholarly journals Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

2020 ◽  
Author(s):  
Lei Liu ◽  
Hongmei Qi ◽  
Pengcheng Suo ◽  
Huai Lin ◽  
Siyi Wang ◽  
...  
Keyword(s):  
Health Risk ◽  
Intestinal Microbiota ◽  
Human Disease ◽  
Alpha Diversity ◽  
Intestinal Microbiome ◽  
Limited Information ◽  
Combination Effects ◽  
Human Intestinal Microbiota ◽  
Future Work

Abstract Background: Antibiotics are emerging toxic contaminant that have potential public health risk worldwide, which also would cause human intestinal microbial disorder and develop multiple human diseases. However, to date, the combination effects of antibiotics on human intestinal microbiota dysbiosis and related health risk are not fully understood. Moreover, there is limited information on using probiotics or synbiotics for restoration of intestinal microbiome affected by antibiotics. Therefore, this study evaluated the in vitro ability of combined effects of amoxicillin (Amx) and gentamycin (Gen), and the restoration effects of probiotics or synbiotics on ARGs as well as human disease-related pathways in the simulated human gut.Results: This study indicated that the combination exposure of Amx and Gen was confirmed to promote the increase of most ARGs and the disease-related pathways, which may be better restored by probiotics treatment. The results of the alpha diversity of the combined antibiotics exposure or the recovery microbial community showed no difference from the control. However, the beta diversity results indicated their differences, and the ascending colon sample recovered better under natural condition while the descending colon sample recovered better after probiotics treatment. Combination effects on the genetic level might attribute to microbiota shift, which were explained well by the phenomenon that Escherichia/Shigella was positively associated with the ARGs, and Klebsiella and Escherichia/Shigella were positively related to the human disease-related pathways.Conclusion: These results might be valuable to direct the future work and opened up new perspectives to address the direct effects of combine antibiotics on the intestinal microbiota and find a promising strategy to restore the antibiotics associated dysbiosis of gut microbiota.

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