In vitro extraction and fermentation of polyphenols from grape seeds (Vitis vinifera) by human intestinal microbiota

2016 ◽  
Vol 7 (4) ◽  
pp. 1959-1967 ◽  
Author(s):  
Li Zhou ◽  
Wei Wang ◽  
Jun Huang ◽  
Yu Ding ◽  
Zhouqiang Pan ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Intestinal Microbiota ◽  
Extraction Yield ◽  
Liquid Extraction ◽  
Pressurized Liquid Extraction ◽  
Grape Seeds ◽  
Total Polyphenols ◽  
Human Intestinal Microbiota

The effects of several parameters on the extraction yield of total polyphenols from grape seeds by pressurized liquid extraction were investigated.

scholarly journals Impact of Chronic Tetracycline Exposure on Human Intestinal Microbiota in a Continuous Flow Bioreactor Model

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 886
Author(s):  
Youngbeom Ahn ◽  
Ji Young Jung ◽  
Ohgew Kweon ◽  
Brian T. Veach ◽  
Sangeeta Khare ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Intestinal Microbiota ◽  
Continuous Flow ◽  
Antimicrobial Drug ◽  
Bioreactor Culture ◽  
Analysis Techniques ◽  
Human Intestinal Microbiota ◽  
Traditional Cultures ◽  
The Impact

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.

Fermentation in vitro of EGCG, GCG and EGCG3"Me isolated from Oolong tea by human intestinal microbiota

2013 ◽  
Vol 54 (2) ◽  
pp. 1589-1595 ◽  
Author(s):  
Xin Zhang ◽  
Xiuling Zhu ◽  
Yongkang Sun ◽  
Bing Hu ◽  
Yi Sun ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Oolong Tea ◽  
Human Intestinal Microbiota

Study of solid–liquid extraction kinetics of total polyphenols from grape seeds

2007 ◽  
Vol 81 (1) ◽  
pp. 236-242 ◽  
Author(s):  
Ana Bucić-Kojić ◽  
Mirela Planinić ◽  
Srećko Tomas ◽  
Mate Bilić ◽  
Darko Velić
Keyword(s):  
Liquid Extraction ◽  
Extraction Kinetics ◽  
Grape Seeds ◽  
Total Polyphenols ◽  
Solid Liquid Extraction ◽  
Solid Liquid ◽  
Kinetics Of

scholarly journals In Vitro Bioconversion of Polyphenols from Black Tea and Red Wine/Grape Juice by Human Intestinal Microbiota Displays Strong Interindividual Variability

2010 ◽  
Vol 58 (18) ◽  
pp. 10236-10246 ◽  
Author(s):  
Gabriele Gross ◽  
Doris M. Jacobs ◽  
Sonja Peters ◽  
Sam Possemiers ◽  
John van Duynhoven ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Red Wine ◽  
Interindividual Variability ◽  
Grape Juice ◽  
Black Tea ◽  
Wine Grape ◽  
Human Intestinal Microbiota

scholarly journals Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

2020 ◽  
Author(s):  
Lei Liu ◽  
Hongmei Qi ◽  
Pengcheng Suo ◽  
Huai Lin ◽  
Siyi Wang ◽  
...  
Keyword(s):  
Health Risk ◽  
Intestinal Microbiota ◽  
Human Disease ◽  
Alpha Diversity ◽  
Intestinal Microbiome ◽  
Limited Information ◽  
Combination Effects ◽  
Human Intestinal Microbiota ◽  
Future Work

Abstract Background: Antibiotics are emerging toxic contaminant that have potential public health risk worldwide, which also would cause human intestinal microbial disorder and develop multiple human diseases. However, to date, the combination effects of antibiotics on human intestinal microbiota dysbiosis and related health risk are not fully understood. Moreover, there is limited information on using probiotics or synbiotics for restoration of intestinal microbiome affected by antibiotics. Therefore, this study evaluated the in vitro ability of combined effects of amoxicillin (Amx) and gentamycin (Gen), and the restoration effects of probiotics or synbiotics on ARGs as well as human disease-related pathways in the simulated human gut.Results: This study indicated that the combination exposure of Amx and Gen was confirmed to promote the increase of most ARGs and the disease-related pathways, which may be better restored by probiotics treatment. The results of the alpha diversity of the combined antibiotics exposure or the recovery microbial community showed no difference from the control. However, the beta diversity results indicated their differences, and the ascending colon sample recovered better under natural condition while the descending colon sample recovered better after probiotics treatment. Combination effects on the genetic level might attribute to microbiota shift, which were explained well by the phenomenon that Escherichia/Shigella was positively associated with the ARGs, and Klebsiella and Escherichia/Shigella were positively related to the human disease-related pathways.Conclusion: These results might be valuable to direct the future work and opened up new perspectives to address the direct effects of combine antibiotics on the intestinal microbiota and find a promising strategy to restore the antibiotics associated dysbiosis of gut microbiota.

scholarly journals Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12513
Author(s):  
Jin-Yi Wan ◽  
Jing-Xuan Wan ◽  
Shilei Wang ◽  
Xiaolu Wang ◽  
Wenqian Guo ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Ultra Performance Liquid Chromatography ◽  
Bark Extract ◽  
Chemical Components ◽  
Root Bark ◽  
Chemical Profiling ◽  
Human Intestinal Microbiota ◽  
Oplopanax Elatus ◽  
Main Components

Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.

Interactions between hawthorn extract WS® 1442 and human intestinal microbiota in vitro

2017 ◽  
Author(s):  
EM Pferschy-Wenzig ◽  
A Roßmann ◽  
K Koskinen ◽  
K Ardjomand-Woelkart ◽  
G Meng ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Human Intestinal Microbiota ◽  
Hawthorn Extract

Accelerated Extraction and Analysis of Ethyl Glucuronide in Hair by Means of Pressurized Liquid Extraction Followed by Liquid Chromatography–Tandem Mass Spectrometry Determination

2020 ◽  
Author(s):  
Flaminia Vincenti ◽  
Camilla Montesano ◽  
Eleonora Oliva ◽  
Federico Fanti ◽  
Marco Vincenti ◽  
...  
Keyword(s):  
Inner Core ◽  
Incubation Temperature ◽  
Solid Phase ◽  
Extraction Yield ◽  
Liquid Extraction ◽  
Ethyl Glucuronide ◽  
Pressurized Liquid Extraction ◽  
New Method ◽  
Extraction Solvent ◽  
Hair Samples

Abstract The measurement of ethyl glucuronide (EtG) in hair is an established practice to evaluate alcohol consumption habits of the donors; nevertheless, analytical variability has shown to be an important factor to be considered: measured EtG values can vary significantly as a consequence of analyte washout during decontamination, pulverization of samples, extraction solvent and incubation temperature. In the present study, we described a new method for automated hair decontamination and EtG extraction from the inner core of the hair by using pressurized liquid extraction (PLE), followed by solid-phase extraction (SPE) cleanup; validation was performed according to SWGTOX guidelines. The extraction efficiency of the new method was evaluated by comparing the results with those obtained by a validated and ISO/IEC 17025:2005 accredited method; an average positive difference of + 32% was observed when the extraction was performed by PLE. The effect of hair pulverization was also studied and a good correlation between cut and milled hair was observed, implying that PLE allowed a highly efficient extraction of EtG from the inner keratin core of the hair, no matter if it has been cut or pulverized. Finally, to verify the results, paired aliquots of 27 real hair samples were analyzed with both PLE and a protocol optimized by design-of-experiment strategies planned to maximize the extraction yield; in this case, a comparable efficiency was observed, suggesting that exhaustive EtG extraction was obtained with both approaches. This finding opens new perspectives in the eligible protocols devoted to hair EtG analysis, in terms of speed, automation and reproducibility.

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