scholarly journals In Vitro Bioconversion of Polyphenols from Black Tea and Red Wine/Grape Juice by Human Intestinal Microbiota Displays Strong Interindividual Variability

2010 ◽  
Vol 58 (18) ◽  
pp. 10236-10246 ◽  
Author(s):  
Gabriele Gross ◽  
Doris M. Jacobs ◽  
Sonja Peters ◽  
Sam Possemiers ◽  
John van Duynhoven ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Red Wine ◽  
Interindividual Variability ◽  
Grape Juice ◽  
Black Tea ◽  
Wine Grape ◽  
Human Intestinal Microbiota

Impact of polyphenols from black tea and red wine/grape juice on a gut model microbiome

2013 ◽  
Vol 53 (2) ◽  
pp. 659-669 ◽  
Author(s):  
Robèr A. Kemperman ◽  
Gabriele Gross ◽  
Stanilas Mondot ◽  
Sam Possemiers ◽  
Massimo Marzorati ◽  
...  
Keyword(s):  
Red Wine ◽  
Grape Juice ◽  
Black Tea ◽  
Wine Grape

scholarly journals The modulatory effect of polyphenols from green tea, oolong tea and black tea on human intestinal microbiota in vitro

2017 ◽  
Vol 55 (1) ◽  
pp. 399-407 ◽  
Author(s):  
Hanyang Sun ◽  
Yuhui Chen ◽  
Mei Cheng ◽  
Xin Zhang ◽  
Xiaojie Zheng ◽  
...  
Keyword(s):  
Green Tea ◽  
Intestinal Microbiota ◽  
Black Tea ◽  
Oolong Tea ◽  
Human Intestinal Microbiota

Gut Microbial Metabolism of Polyphenols from Black Tea and Red Wine/Grape Juice Is Source-Specific and Colon-Region Dependent

2012 ◽  
Vol 60 (45) ◽  
pp. 11331-11342 ◽  
Author(s):  
F. A. van Dorsten ◽  
S. Peters ◽  
G. Gross ◽  
V. Gomez-Roldan ◽  
M. Klinkenberg ◽  
...  
Keyword(s):  
Red Wine ◽  
Grape Juice ◽  
Black Tea ◽  
Microbial Metabolism ◽  
Wine Grape

scholarly journals Impact of Chronic Tetracycline Exposure on Human Intestinal Microbiota in a Continuous Flow Bioreactor Model

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 886
Author(s):  
Youngbeom Ahn ◽  
Ji Young Jung ◽  
Ohgew Kweon ◽  
Brian T. Veach ◽  
Sangeeta Khare ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Intestinal Microbiota ◽  
Continuous Flow ◽  
Antimicrobial Drug ◽  
Bioreactor Culture ◽  
Analysis Techniques ◽  
Human Intestinal Microbiota ◽  
Traditional Cultures ◽  
The Impact

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.

Fermentation in vitro of EGCG, GCG and EGCG3"Me isolated from Oolong tea by human intestinal microbiota

2013 ◽  
Vol 54 (2) ◽  
pp. 1589-1595 ◽  
Author(s):  
Xin Zhang ◽  
Xiuling Zhu ◽  
Yongkang Sun ◽  
Bing Hu ◽  
Yi Sun ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Oolong Tea ◽  
Human Intestinal Microbiota

Salivary Proteins Interact with Dietary Constituents to Modulate Tooth Staining

2005 ◽  
Vol 84 (1) ◽  
pp. 73-78 ◽  
Author(s):  
G.B. Proctor ◽  
R. Pramanik ◽  
G.H. Carpenter ◽  
G.D. Rees
Keyword(s):  
Red Wine ◽  
Black Tea ◽  
Salivary Protein ◽  
Salivary Proteins ◽  
Parotid Saliva ◽  
Grape Skin ◽  
Dietary Components ◽  
Black Tea Polyphenols ◽  
Proline Rich Protein

Dietary components rich in polyphenols—for example, tea and red wine—are thought to cause tooth staining. In the present study, hydroxyapatite was used as a model of enamel for study of the influence of salivary proteins on the binding of different polyphenols to hydroxyapatite in vitro. Neither salivary protein pellicles nor salivary proteins in solution significantly altered the binding of the small polyphenol epigallocatechin to hydroxyapatite. However, hydroxyapatite binding of anthocyanin, a small grape-skin-derived polyphenol, or the larger polyphenols of black tea was increased by the presence of salivary proteins, either as a pellicle or in solution. Proline-rich proteins were enriched from parotid saliva and found to increase binding of anthocyanin and black tea polyphenols to hydroxyapatite, while enriched histatins did not increase binding. It is concluded that some salivary proteins, including proline-rich protein, can mediate increased staining of enamel by red-wine- and black-tea-derived polyphenols.

In vitro extraction and fermentation of polyphenols from grape seeds (Vitis vinifera) by human intestinal microbiota

2016 ◽  
Vol 7 (4) ◽  
pp. 1959-1967 ◽  
Author(s):  
Li Zhou ◽  
Wei Wang ◽  
Jun Huang ◽  
Yu Ding ◽  
Zhouqiang Pan ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Intestinal Microbiota ◽  
Extraction Yield ◽  
Liquid Extraction ◽  
Pressurized Liquid Extraction ◽  
Grape Seeds ◽  
Total Polyphenols ◽  
Human Intestinal Microbiota

The effects of several parameters on the extraction yield of total polyphenols from grape seeds by pressurized liquid extraction were investigated.

scholarly journals Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

2020 ◽  
Author(s):  
Lei Liu ◽  
Hongmei Qi ◽  
Pengcheng Suo ◽  
Huai Lin ◽  
Siyi Wang ◽  
...  
Keyword(s):  
Health Risk ◽  
Intestinal Microbiota ◽  
Human Disease ◽  
Alpha Diversity ◽  
Intestinal Microbiome ◽  
Limited Information ◽  
Combination Effects ◽  
Human Intestinal Microbiota ◽  
Future Work

Abstract Background: Antibiotics are emerging toxic contaminant that have potential public health risk worldwide, which also would cause human intestinal microbial disorder and develop multiple human diseases. However, to date, the combination effects of antibiotics on human intestinal microbiota dysbiosis and related health risk are not fully understood. Moreover, there is limited information on using probiotics or synbiotics for restoration of intestinal microbiome affected by antibiotics. Therefore, this study evaluated the in vitro ability of combined effects of amoxicillin (Amx) and gentamycin (Gen), and the restoration effects of probiotics or synbiotics on ARGs as well as human disease-related pathways in the simulated human gut.Results: This study indicated that the combination exposure of Amx and Gen was confirmed to promote the increase of most ARGs and the disease-related pathways, which may be better restored by probiotics treatment. The results of the alpha diversity of the combined antibiotics exposure or the recovery microbial community showed no difference from the control. However, the beta diversity results indicated their differences, and the ascending colon sample recovered better under natural condition while the descending colon sample recovered better after probiotics treatment. Combination effects on the genetic level might attribute to microbiota shift, which were explained well by the phenomenon that Escherichia/Shigella was positively associated with the ARGs, and Klebsiella and Escherichia/Shigella were positively related to the human disease-related pathways.Conclusion: These results might be valuable to direct the future work and opened up new perspectives to address the direct effects of combine antibiotics on the intestinal microbiota and find a promising strategy to restore the antibiotics associated dysbiosis of gut microbiota.

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