An UPLC-MS/MS Method for the Simultaneous Identification and Quantitation of Cell Wall Phenolics in Brassica napus Seeds

2013 ◽  
Vol 61 (6) ◽  
pp. 1219-1227 ◽  
Author(s):  
Andrej Frolov ◽  
Anja Henning ◽  
Christoph Böttcher ◽  
Alain Tissier ◽  
Dieter Strack
Keyword(s):  
Cell Wall ◽  
Brassica Napus ◽  
Simultaneous Identification

scholarly journals Low-Boron Tolerance Strategies Involving Pectin-Mediated Cell Wall Mechanical Properties in Brassica napus

2017 ◽  
Vol 58 (11) ◽  
pp. 1991-2005 ◽  
Author(s):  
Ting Zhou ◽  
Yingpeng Hua ◽  
Baocai Zhang ◽  
Xiuqing Zhang ◽  
Yihua Zhou ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Wall ◽  
Brassica Napus ◽  
Boron Tolerance

Characterisation of cell wall polysaccharides from rapeseed (Brassica napus) meal

2013 ◽  
Vol 98 (2) ◽  
pp. 1650-1656 ◽  
Author(s):  
Annemieke M. Pustjens ◽  
Henk A. Schols ◽  
Mirjam A. Kabel ◽  
Harry Gruppen
Keyword(s):  
Cell Wall ◽  
Brassica Napus ◽  
Cell Wall Polysaccharides

scholarly journals Phytochemical fingerprinting of vegetable Brassica oleracea and Brassica napus by simultaneous identification of glucosinolates and phenolics

2011 ◽  
Vol 22 (2) ◽  
pp. 144-152 ◽  
Author(s):  
Pablo Velasco ◽  
Marta Francisco ◽  
Diego A. Moreno ◽  
Federico Ferreres ◽  
Cristina García-Viguera ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Simultaneous Identification ◽  
Vegetable Brassica

Transcripts of a gene encoding a putative cell wall-plasma membrane linker protein are specifically cold-induced in Brassica napus

1996 ◽  
Vol 31 (4) ◽  
pp. 771-781 ◽  
Author(s):  
William Goodwin ◽  
Jacqueline A. Pallas ◽  
Gareth I. Jenkins
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Brassica Napus ◽  
Gene Encoding

Lignin composition and timing of cell wall lignification are involved in Brassica napus resistance to Sclerotinia sclerotiorum stem rot

2021 ◽  
Author(s):  
Andreas von Tiedemann ◽  
Birger Koopmann ◽  
Kerstin Hoech
Keyword(s):  
Cell Wall ◽  
Brassica Napus ◽  
Sclerotinia Sclerotiorum ◽  
Plant Defence ◽  
Phenylpropanoid Pathway ◽  
Stem Rot ◽  
Phenylpropanoid Metabolism ◽  
Sclerotinia Stem Rot ◽  
Cell Wall Modification ◽  
Lignin Deposition

Sclerotinia stem rot (SSR) is an economically and globally significant disease in oilseed rape (Brassica napus L.) caused by the necrotrophic ascomycete Sclerotinia sclerotiorum. This study explored the role of cell wall reinforcement by lignin as a relevant factor for effective plant defence against attack of this pathogen. Expression of key genes in the phenylpropanoid pathway and the induced synthesis of lignin in infected stem tissues were investigated in a study comparing a susceptible (Loras) and a moderately resistant cultivar (Zhongyou 821, ZY821). Data revealed an earlier and more rapid defence activation in ZY821 through up-regulation of transcript levels of genes related to key steps in the phenylpropanoid pathway associated with enhanced lignin deposition in the resistant B. napus genotype. Expression level of BnCAD5, encoding a cinnamyl alcohol dehydrogenase, responsible for conversion of monolignol to lignin, was more rapidly up-regulated in ZY821 than Loras. The similar expression pattern of BnCAD5 and the gene BnF5H, encoding for the ferulate-5-hydroxylase which catalyses the synthesis of syringyl (S) lignin precursors, suggests that BnCAD5 is involved in the S lignin formation. Histological observations confirmed these results, showing an earlier increase of S lignin deposition in the infected resistant genotype. Deposition of guaiacyl (G) lignin was detected in both genotypes and is thus considered a component of basal, cultivar-independent defence response of B. napus to stem rot. The results indicate the importance of cell wall modification for quantitative stem rot resistance by responses in the phenylpropanoid metabolism generating distinct lignin types on different temporal scales.

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