Temporary inhibition of cell wall synthesis improves the transient expression of the GUS gene in Brassica napus mesophyll protoplasts

1990 ◽  
Vol 9 (2) ◽  
Author(s):  
Marc Chapel ◽  
Kristina Glimelius
Keyword(s):  
Cell Wall ◽  
Brassica Napus ◽  
Transient Expression ◽  
Gus Gene ◽  
Cell Wall Synthesis ◽  
Mesophyll Protoplasts ◽  
Temporary Inhibition

scholarly journals The Effect of Hydroxylysine on Cell Wall Synthesis and Cell Stability in Streptococcus faecalis

1962 ◽  
Vol 237 (4) ◽  
pp. 1198-1202
Author(s):  
W. Grady Smith ◽  
Mary Newman ◽  
Franklin R. Leach ◽  
L.M. Henderson
Keyword(s):  
Cell Wall ◽  
Cell Wall Synthesis ◽  
Streptococcus Faecalis ◽  
Cell Stability

scholarly journals Direct Interaction of Polar Scaffolding Protein Wag31 with Nucleoid-Associated Protein Rv3852 Regulates Its Polar Localization

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1558
Author(s):  
Rajni Garg ◽  
Chinmay Anand ◽  
Sohini Ganguly ◽  
Sandhya Rao ◽  
Rinkee Verma ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Envelope ◽  
Transmembrane Helix ◽  
Ectopic Expression ◽  
Direct Interaction ◽  
Fine Tuning ◽  
Scaffolding Protein ◽  
Physical Interaction ◽  
Cell Wall Synthesis ◽  
Interaction Approach

Rv3852 is a unique nucleoid-associated protein (NAP) found exclusively in Mycobacterium tuberculosis (Mtb) and closely related species. Although annotated as H-NS, we showed previously that it is very different from H-NS in its properties and is distinct from other NAPs, anchoring to cell membrane by virtue of possessing a C-terminal transmembrane helix. Here, we investigated the role of Rv3852 in Mtb in organizing architecture or synthesis machinery of cell wall by protein–protein interaction approach. We demonstrated a direct physical interaction of Rv3852 with Wag31, an important cell shape and cell wall integrity determinant essential in Mtb. Wag31 localizes to the cell poles and possibly acts as a scaffold for cell wall synthesis proteins, resulting in polar cell growth in Mtb. Ectopic expression of Rv3852 in M. smegmatis resulted in its interaction with Wag31 orthologue DivIVAMsm. Binding of the NAP to Wag31 appears to be necessary for fine-tuning Wag31 localization to the cell poles, enabling complex cell wall synthesis in Mtb. In Rv3852 knockout background, Wag31 is mislocalized resulting in disturbed nascent peptidoglycan synthesis, suggesting that the NAP acts as a driver for localization of Wag31 to the cell poles. While this novel association between these two proteins presents one of the mechanisms to structure the elaborate multi-layered cell envelope of Mtb, it also exemplifies a new function for a NAP in mycobacteria.

scholarly journals Quantitative confocal imaging method for analyzing cellulose dynamics during cell wall regeneration in Arabidopsis mesophyll protoplasts

Plant Direct ◽  
2017 ◽  
Vol 1 (6) ◽  
pp. e00021 ◽  
Author(s):  
Hiroaki Kuki ◽  
Takumi Higaki ◽  
Ryusuke Yokoyama ◽  
Takeshi Kuroha ◽  
Naoki Shinohara ◽  
...  
Keyword(s):  
Cell Wall ◽  
Confocal Imaging ◽  
Imaging Method ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration
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