scholarly journals Phytochemical fingerprinting of vegetable Brassica oleracea and Brassica napus by simultaneous identification of glucosinolates and phenolics

2011 ◽  
Vol 22 (2) ◽  
pp. 144-152 ◽  
Author(s):  
Pablo Velasco ◽  
Marta Francisco ◽  
Diego A. Moreno ◽  
Federico Ferreres ◽  
Cristina García-Viguera ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Simultaneous Identification ◽  
Vegetable Brassica

Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa Cytoplasm

2010 ◽  
Vol 36 (8) ◽  
pp. 1280-1285 ◽  
Author(s):  
Jun LI ◽  
Li-Xia LUO ◽  
Zhuan WANG ◽  
Jun LI ◽  
Kun-Rong CHEN ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Brassica Rapa

scholarly journals A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L.

2011 ◽  
Vol 42 (No. 3) ◽  
pp. 103-110 ◽  
Author(s):  
N.D. Kaur ◽  
M. Vyvadilová ◽  
M. Klíma ◽  
M. Bechyně
Keyword(s):  
Brassica Napus ◽  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Growth Regulators ◽  
Protoplast Culture ◽  
Treatment Time ◽  
Simple Procedure ◽  
Enzyme Treatment ◽  
Induction Medium ◽  
Brassica Napus L

An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8&ndash;11.2 &times; 10<sup>4 </sup>protoplasts/ml in darkness at 25&deg;C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures were diluted with low osmotic medium on 7<sup>th</sup> and 11<sup>th</sup> day. The frequency of dividing cells was about 80% in B. oleracea and 50% in B. napus. After one month, the microcalli of approximately 0.5&ndash;1 mm in size were transferred into an induction medium with various combinations of growth regulators. Minimum duration of enzyme treatment time and extended dark period in the initial phase of culture increased the survival rate of protoplasts. Organogenesis started when the calli enlarged in size on an induction medium (1 mg/l NAA, 0.02 mg/l GA<sub>3</sub>, 1 mg/l 2iP) with 2% sucrose and 0.8% agar. Regeneration frequency of calli was found to be 69&ndash;75% in B. oleracea and 2&ndash;3% in B. napus. Well-developed shoots were transferred for rooting to a half-strength MS medium without growth regulators. More than 100 B. oleracea regenerants were transferred into soil, and they produced normal heads and set seeds. This very simple procedure is efficient and suitable mainly for B. oleracea var. botrytis and represents a background for fusion experiments. &nbsp;

An UPLC-MS/MS Method for the Simultaneous Identification and Quantitation of Cell Wall Phenolics in Brassica napus Seeds

2013 ◽  
Vol 61 (6) ◽  
pp. 1219-1227 ◽  
Author(s):  
Andrej Frolov ◽  
Anja Henning ◽  
Christoph Böttcher ◽  
Alain Tissier ◽  
Dieter Strack
Keyword(s):  
Cell Wall ◽  
Brassica Napus ◽  
Simultaneous Identification

scholarly journals Spontaneous gene flow from rapeseed ( Brassica napus ) to wild Brassica oleracea

2006 ◽  
Vol 273 (1605) ◽  
pp. 3111-3115 ◽  
Author(s):  
Caroline S Ford ◽  
Joël Allainguillaume ◽  
Phil Grilli-Chantler ◽  
Giulia Cuccato ◽  
Charlotte J Allender ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Gene Flow ◽  
Brassica Napus ◽  
Microsatellite Markers ◽  
Brassica Oleracea ◽  
Brassica Rapa ◽  
Environmental Risks ◽  
Wild Relatives ◽  
Similar Species ◽  
In The Wild

Research on the environmental risks of gene flow from genetically modified (GM) crops to wild relatives has traditionally emphasized recipients yielding most hybrids. For GM rapeseed ( Brassica napus ), interest has centred on the ‘frequently hybridizing’ Brassica rapa over relatives such as Brassica oleracea , where spontaneous hybrids are unreported in the wild. In two sites, where rapeseed and wild B. oleracea grow together, we used flow cytometry and crop-specific microsatellite markers to identify one triploid F 1 hybrid, together with nine diploid and two near triploid introgressants. Given the newly discovered capacity for spontaneous introgression into B. oleracea , we then surveyed associated flora and fauna to evaluate the capacity of both recipients to harm cohabitant species with acknowledged conservational importance. Only B. oleracea occupies rich communities containing species afforded legislative protection; these include one rare micromoth species that feeds on B. oleracea and warrants further assessment. We conclude that increased attention should now focus on B. oleracea and similar species that yield few crop-hybrids, but possess scope to affect rare or endangered associates.

Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step

2015 ◽  
Vol 128 (4) ◽  
pp. 639-644 ◽  
Author(s):  
Jiaqin Mei ◽  
Yao Liu ◽  
Dayong Wei ◽  
Benjamin Wittkop ◽  
Yijuan Ding ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Wild Relative

The proteins of green forage plants: III. The proteins of forage plants of the natural order Cruciferae (genus Brassica). Comparison with colzalin, a globulin from rapeseed

1927 ◽  
Vol 17 (1) ◽  
pp. 33-40 ◽  
Author(s):  
W. L. Davies
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Natural Order ◽  
Forage Plants ◽  
Stems And Leaves ◽  
Leaves And Roots

In a previous paper (Davies, 1926) the author studied the differences in the compositions of protoplasmic proteins of plants within a Natural Order (Leguminosae). The Natural Order Cruciferae afforded a means of developing the study in the direction not only of ascertaining differences, if any, of protoplasmic protein of plants within a genus, but also of differences possible within a species. Thus proteins were studied from the following varieties of the cabbage species (Brassica oleracea L.): Cabbage (B. oleracea var. capitata), Marrow stem kale (B. oleracea var.) from the stems and leaves separately, Kohl rabi (B. oleracea var. caulorapa). Also, the proteins from the leaves and roots respectively of white turnips (B. napo-brassica) were isolated and studied. In order to compare the protoplasmic proteins with those of seed of a plant of the same species, a globulin from rapeseed (seed of Brassica Napus L.) was prepared and analysed.

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