Event-Specific Quantitative Detection of Nine Genetically Modified Maizes Using One Novel Standard Reference Molecule

2007 ◽  
Vol 55 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Litao Yang ◽  
Jinchao Guo ◽  
Aihu Pan ◽  
Haibo Zhang ◽  
Kewei Zhang ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Quantitative Detection ◽  
Reference Molecule

Quantitative Detection System for Maize Sample Containing Combined-Trait Genetically Modified Maize

2005 ◽  
Vol 77 (22) ◽  
pp. 7421-7428 ◽  
Author(s):  
Hiroshi Akiyama ◽  
Takahiro Watanabe ◽  
Kaoru Wakabayashi ◽  
Shinsuke Nakade ◽  
Shuji Yasui ◽  
...  
Keyword(s):  
Detection System ◽  
Genetically Modified ◽  
Quantitative Detection ◽  
Genetically Modified Maize ◽  
Maize Sample

Qualitative and Quantitative Detection of Protein and Genetic Traits in Genetically Modified Food

2004 ◽  
Vol 20 (3) ◽  
pp. 275-296 ◽  
Author(s):  
P. Markoulatos ◽  
N. Siafakas ◽  
A. Papathoma ◽  
E. Nerantzis ◽  
B. Betzios ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Quantitative Detection ◽  
Genetically Modified Food ◽  
Genetic Traits ◽  
Qualitative And Quantitative

scholarly journals Establishment and In-House Validation of Simplex and Duplex PCR Methods for Event-Specific Detection of Maize SYN-E3272-5 Using a New Reference Molecule

2010 ◽  
Vol 93 (2) ◽  
pp. 663-675
Author(s):  
Kailin Shen ◽  
Xiang Li ◽  
Shu Wang ◽  
Yingjie Pan ◽  
Zhiyi Shi ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Genetically Modified Organisms ◽  
Insertion Site ◽  
Quantitative Detection ◽  
Duplex System ◽  
Detection Techniques ◽  
Qualitative And Quantitative ◽  
Reference Molecule ◽  
Gm Maize ◽  
Pcr Assays

Abstract Despite rapid developments in the detection techniques for genetically modified organisms (GMOs), the event-specific PCR method with high specificity is still the most used technique. In this study, event-specific simplex and duplex qualitative and quantitative detection systems were developed targeting the 3 insertion site of GM maize SYN-E3272-5 (3272) construct. A reference molecule p3272 was constructed to act as positive control and as calibrator for quantitative analysis. The LOD for simplex and duplex qualitative PCR assays was 10 copies of p3272 control DNA. LOD and the LOQ for simplex and duplex quantitative PCR assays were 10 and 25 copies of p3272 DNA, respectively. Furthermore, four practical GM maize samples were quantified using the established simplex and duplex quantitative PCR systems by in-house validation. Results from five operators showed that the bias ranged from 3.44 to 17.24 in the simplex system and from 0.42 to 16.06 in the duplex system, respectively. These results demonstrated that the established event-specific simplex and duplex qualitative and quantitative PCR systems combined with the reference molecule p3272 are suitable for the detection of GM maize 3272 and its derived products.

Qualitative and Quantitative PCR-Based Detection Methods for Authorized Genetically Modified Cotton Events in India

2014 ◽  
Vol 97 (5) ◽  
pp. 1299-1309 ◽  
Author(s):  
Rashmi Chhabra ◽  
Gurinder Jit Randhawa ◽  
Rajesh K Bhoge ◽  
Monika Singh
Keyword(s):  
Genetically Modified ◽  
Cost Effective ◽  
Detection Methods ◽  
Quantitative Detection ◽  
Bt Cotton ◽  
Pcr Assay ◽  
Genetically Modified Cotton ◽  
Detection Assay ◽  
Endogenous Reference ◽  
The Cost

Abstract Qualitative diagnostics for all five commercialized genetically modified (GM) cotton events for insect resistance in India is being reported for the first time in this paper. The cost-effective and robust multiplex PCR (MPCR)-based detection assay, distinguishing the insect resistant transgenic Bt cotton events, viz., MON531, MON15985, Event 1, GFM-cry1A, and MLS-9124, has been developed. This decaplex PCR assay targets nine transgenic elements, viz., sequences of four transgenes, three transgene constructs, and two event-specific sequences along with one endogenous reference gene. The LOD of the qualitative MPCR assay was up to 0.1%. A quantitative detection method for four widely commercially cultivated GM cotton events, namely, MON531, MON15985, Event 1, and GFM-cry1A, covering 99.5% of the total area under GM cultivation in the country, is also reported. A construct-specific real-time PCR assay has been developed for quantification of these GM cotton events with LOQ <0.05% and LOD <0.025%. The developed assays will be of great use to screen for the presence/absence of authorized GM cotton events in unknown samples and to check the authenticity of GM cotton seed samples.

Event-specific quantitative detection of genetically modified wheat B72-8-11 based on the 3′ flanking sequence

2014 ◽  
Vol 240 (4) ◽  
pp. 775-782 ◽  
Author(s):  
Minghui Zhang ◽  
Yanbo Yu ◽  
Xuejun Gao ◽  
Kejian Zhang ◽  
Fengxia Luan ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Quantitative Detection ◽  
Flanking Sequence

scholarly journals Semi-quantitative detection of genetically modified grains based on CaMV 35S promoter amplification

2000 ◽  
Vol 3 (2) ◽  
Author(s):  
Alejandro C. Tozzini ◽  
M. Carolina Martínez ◽  
M. Florencia Lucca ◽  
Cecilia Vázquez Rovere ◽  
Ana Julia Distéfano ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Camv 35S Promoter ◽  
Quantitative Detection ◽  
35S Promoter ◽  
Camv 35S
Sign in / Sign up

Export Citation Format

Share Document