Ultrasensitive Detection of Genetically Modified Maize DNA by Capillary Gel Electrophoresis with Laser-Induced Fluorescence Using Different Fluorescent Intercalating Dyes

2002 ◽  
Vol 50 (16) ◽  
pp. 4497-4502 ◽  
Author(s):  
Virginia García-Cañas ◽  
Ramón González ◽  
Alejandro Cifuentes
Keyword(s):  
Gel Electrophoresis ◽  
Genetically Modified ◽  
Laser Induced Fluorescence ◽  
Ultrasensitive Detection ◽  
Genetically Modified Maize ◽  
Capillary Gel Electrophoresis

Quantification of green fluorescent protein-(GFP-) tagged membrane proteins by capillary gel electrophoresis

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3648-3655
Author(s):  
Azeem Danish ◽  
Sang-Yong Lee ◽  
Christa E. Müller
Keyword(s):  
Green Fluorescent Protein ◽  
Membrane Proteins ◽  
Fluorescence Detection ◽  
Gel Electrophoresis ◽  
Fluorescent Protein ◽  
Laser Induced Fluorescence ◽  
Capillary Gel Electrophoresis ◽  
Laser Induced Fluorescence Detection ◽  
Green Fluorescent

A fast and robust procedure for the quantification of GFP-tagged membrane proteins in cell homogenates was developed employing capillary gel electrophoresis coupled to laser-induced fluorescence detection (CGE-LIF).

Detection and Identification of Transgenic Elements by Fluorescent-PCR-Based Capillary Gel Electrophoresis in Genetically Modified Cotton and Soybean

2014 ◽  
Vol 97 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Sanjay Basak ◽  
Nasreen Z Ehtesham ◽  
Boindala Sesikeran ◽  
Sudip Ghosh
Keyword(s):  
Multiplex Pcr ◽  
Dna Sequences ◽  
Gel Electrophoresis ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Bt Cotton ◽  
Regulatory Requirement ◽  
Sequencing Platform ◽  
Capillary Gel Electrophoresis ◽  
Multiplex Pcr Assay

Abstract A detection method for genetically modified foods is an essential regulatory requirement for many countries. The present study is aimed at developing a qualitative method for detection of genetically modified organisms by combining PCR methodology with capillary gel electrophoresis (PCR-CGE) in a sequencing platform to detect Bacillus thuringiensis (Bt)-cotton (MON 531)and Roundup Ready (RR) soybean (GTS 40-3-2). A sensitive duplex PCR-CGE method was developed in which target DNA sequences (35S and Nos) were separated both by size and color to detect 0.01% Cry1Ac DNA (w/w) in Bt-cotton. A multiplex PCR-CGE method was developed to simultaneously detect fourtargets such as Sad1, Cry1Ac, 35S, and Nos in Bt-cotton. Four novel PCR primers were designed to customize amplicon size for multiplexing for better visualizationof multiple peaks. The LOD for Cry1Ac DNA specific PCR was 0.01% for Bt-cotton. The LOD for multiplex PCR assay was 0.05% for Bt-cotton. A singleplex PCR-CGE method was developed to detect Lec, 35S and Nos in a trace sample of RR soybean grainpowder (0.1%, w/w). This study demonstrates aPCR-CGE-based method for the qualitative detection of35S, Nos and Cry1Ac targets associated with genetically modifiedproducts.

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