Detection of Genetically Modified Maize by the Polymerase Chain Reaction and Capillary Gel Electrophoresis with UV Detection and Laser-Induced Fluorescence

2002 ◽  
Vol 50 (5) ◽  
pp. 1016-1021 ◽  
Author(s):  
Virginia García-Cañas ◽  
Ramón González ◽  
Alejandro Cifuentes
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Genetically Modified ◽  
Laser Induced Fluorescence ◽  
Uv Detection ◽  
Genetically Modified Maize ◽  
Chain Reaction ◽  
Capillary Gel Electrophoresis ◽  
Polymerase Chain

scholarly journals Multiplex Polymerase Chain Reaction-Capillary Gel Electrophoresis: A Promising Tool for GMO ScreeningAssay for Simultaneous Detection of Five Genetically Modified Cotton Events and Species

2009 ◽  
Vol 92 (3) ◽  
pp. 765-772 ◽  
Author(s):  
Anna Nadal ◽  
Teresa Esteve ◽  
Maria Pla
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Gel Electrophoresis ◽  
Multiplex Polymerase Chain Reaction ◽  
Genetically Modified ◽  
Simultaneous Detection ◽  
Chain Reaction ◽  
Capillary Gel Electrophoresis ◽  
Cotton Species ◽  
Polymerase Chain

Abstract A multiplex polymerase chain reaction assay coupled to capillary gel electrophoresis for amplicon identification by size and color (multiplex PCR-CGE-SC) was developed for simultaneous detection of cotton species and 5 events of genetically modified (GM) cotton. Validated real-time-PCR reactions targeting Bollgard, Bollgard II, Roundup Ready, 3006-210-23, and 281-24-236 junction sequences, and the cotton reference gene acp1 were adapted to detect more than half of the European Union-approved individual or stacked GM cotton events in one reaction. The assay was fully specific (<1.7 of false classification rate), with limit of detection values of 0.1 for each event, which were also achieved with simulated mixtures at different relative percentages of targets. The assay was further combined with a second multiplex PCR-CGE-SC assay to allow simultaneous detection of 6 cotton and 5 maize targets (two endogenous genes and 9 GM events) in two multiplex PCRs and a single CGE, making the approach more economic. Besides allowing simultaneous detection of many targets with adequate specificity and sensitivity, the multiplex PCR-CGE-SC approach has high throughput and automation capabilities, while keeping a very simple protocol, e.g., amplification and labeling in one step. Thus, it is an easy and inexpensive tool for initial screening, to be complemented with quantitative assays if necessary.

scholarly journals Applicability of Three Alternative Instruments for Food Authenticity Analysis:GMO Identification

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
A. Burrell ◽  
C. Foy ◽  
M. Burns
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Fitness For Purpose ◽  
Chain Reaction ◽  
Food Authenticity ◽  
Pcr Products ◽  
Polymerase Chain

Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients.

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