Quantification of green fluorescent protein-(GFP-) tagged membrane proteins by capillary gel electrophoresis

Azeem Danish; Sang-Yong Lee; Christa E. Müller

doi:10.1039/c7an00981j

Quantification of green fluorescent protein-(GFP-) tagged membrane proteins by capillary gel electrophoresis

The Analyst ◽

10.1039/c7an00981j ◽

2017 ◽

Vol 142 (19) ◽

pp. 3648-3655

Author(s):

Azeem Danish ◽

Sang-Yong Lee ◽

Christa E. Müller

Keyword(s):

Green Fluorescent Protein ◽

Membrane Proteins ◽

Fluorescence Detection ◽

Gel Electrophoresis ◽

Fluorescent Protein ◽

Laser Induced Fluorescence ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection ◽

Green Fluorescent

A fast and robust procedure for the quantification of GFP-tagged membrane proteins in cell homogenates was developed employing capillary gel electrophoresis coupled to laser-induced fluorescence detection (CGE-LIF).

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Capillary Electrophoresis with Laser-Induced Fluorescence Detection for the Analysis of Free and Immune-Complexed Green Fluorescent Protein

Analytical Biochemistry ◽

10.1006/abio.1997.2242 ◽

1997 ◽

Vol 251 (2) ◽

pp. 210-218 ◽

Cited By ~ 25

Author(s):

Gina M. Korf ◽

James P. Landers ◽

Dennis J. O'kane

Keyword(s):

Capillary Electrophoresis ◽

Green Fluorescent Protein ◽

Fluorescence Detection ◽

Fluorescent Protein ◽

Laser Induced Fluorescence ◽

Laser Induced Fluorescence Detection ◽

Green Fluorescent

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Quantification of green fluorescent protein in cellular supernatant by capillary electrophoresis with laser-induced fluorescence detection for measurement of cell death

Talanta ◽

10.1016/j.talanta.2010.01.041 ◽

2010 ◽

Vol 81 (3) ◽

pp. 948-953 ◽

Cited By ~ 9

Author(s):

Kristian E. Swearingen ◽

Wendy P. Loomis ◽

Benjamin Kehimkar ◽

Brad T. Cookson ◽

Norman J. Dovichi

Keyword(s):

Cell Death ◽

Capillary Electrophoresis ◽

Green Fluorescent Protein ◽

Fluorescence Detection ◽

Fluorescent Protein ◽

Laser Induced Fluorescence ◽

Laser Induced Fluorescence Detection ◽

Green Fluorescent

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Detection ofAequorea victoria Green Fluorescent Protein by Capillary Electrophoresis Laser Induced Fluorescence Detection

Biomedical Chromatography ◽

10.1002/(sici)1099-0801(199707)11:4<205::aid-bmc675>3.0.co;2-# ◽

1997 ◽

Vol 11 (4) ◽

pp. 205-206 ◽

Cited By ~ 10

Author(s):

Douglas B. Craig ◽

Jerome C. Y. Wong ◽

Norman J. Dovichi

Keyword(s):

Capillary Electrophoresis ◽

Green Fluorescent Protein ◽

Fluorescence Detection ◽

Fluorescent Protein ◽

Laser Induced Fluorescence ◽

Laser Induced Fluorescence Detection ◽

Green Fluorescent

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glyXalign: High-throughput migration time alignment preprocessing of electrophoretic data retrieved via multiplexed capillary gel electrophoresis with laser-induced fluorescence detection-based glycoprofiling

Electrophoresis ◽

10.1002/elps.201200696 ◽

2013 ◽

Vol 34 (16) ◽

pp. 2311-2315 ◽

Cited By ~ 10

Author(s):

Alexander Behne ◽

Thilo Muth ◽

Matthias Borowiak ◽

Udo Reichl ◽

Erdmann Rapp

Keyword(s):

Fluorescence Detection ◽

High Throughput ◽

Gel Electrophoresis ◽

Laser Induced Fluorescence ◽

Migration Time ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection ◽

Time Alignment ◽

Electrophoretic Data

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Estimation of protein concentration at high sensitivity using SDS-capillary gel electrophoresis-laser induced fluorescence detection with 3-(2-furoyl)quinoline-2-carboxaldehyde protein labeling

Electrophoresis ◽

10.1002/elps.201600246 ◽

2016 ◽

Vol 37 (22) ◽

pp. 2913-2921 ◽

Cited By ~ 6

Author(s):

Miriam S. Arrell ◽

Franka Kálmán

Keyword(s):

Fluorescence Detection ◽

Gel Electrophoresis ◽

Protein Concentration ◽

High Sensitivity ◽

Laser Induced Fluorescence ◽

Protein Labeling ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection

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High Sensitivity of Laser-Induced Fluorescence Detection in Capillary Gel Electrophoresis for Accurate Apolipoprotein E Genotyping

BioTechniques ◽

10.2144/97224rr03 ◽

1997 ◽

Vol 22 (4) ◽

pp. 736-742 ◽

Cited By ~ 4

Author(s):

A. Schlenck ◽

K. Bohnet ◽

D. Aguillon ◽

C. Lafaurie ◽

G. Siest ◽

...

Keyword(s):

Apolipoprotein E ◽

Fluorescence Detection ◽

Gel Electrophoresis ◽

High Sensitivity ◽

Laser Induced Fluorescence ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection

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Quantitation of Fluorescent Nucleotide Incorporation by Capillary Gel Electrophoresis and Laser-Induced Fluorescence Detection

Analytical Biochemistry ◽

10.1006/abio.1998.3094 ◽

1999 ◽

Vol 269 (1) ◽

pp. 21-31 ◽

Cited By ~ 4

Author(s):

Kenneth A. Cruickshank

Keyword(s):

Fluorescence Detection ◽

Gel Electrophoresis ◽

Laser Induced Fluorescence ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection ◽

Nucleotide Incorporation

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Development of a high-throughput glycoanalysis method for the characterization of oligosaccharides in human milk utilizing multiplexed capillary gel electrophoresis with laser-induced fluorescence detection

Electrophoresis ◽

10.1002/elps.201300016 ◽

2013 ◽

Vol 34 (16) ◽

pp. 2323-2336 ◽

Cited By ~ 30

Author(s):

Robert Kottler ◽

Marko Mank ◽

René Hennig ◽

Beate Müller-Werner ◽

Bernd Stahl ◽

...

Keyword(s):

Human Milk ◽

Fluorescence Detection ◽

High Throughput ◽

Gel Electrophoresis ◽

Laser Induced Fluorescence ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection

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Single-color laser-induced fluorescence detection and capillary gel electrophoresis for DNA sequencing

10.1117/12.44240 ◽

1991 ◽

Cited By ~ 2

Author(s):

Da Yong Chen ◽

Harold Swerdlow ◽

Heather Harke ◽

Jian Z. Zhang ◽

Norman J. Dovichi

Keyword(s):

Dna Sequencing ◽

Fluorescence Detection ◽

Gel Electrophoresis ◽

Laser Induced Fluorescence ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection

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Factors influencing the electrokinetic injection of oligonucleotides in capillary gel electrophoresis when using laser-induced fluorescence detection

Biomedical Chromatography ◽

10.1002/bmc.3036 ◽

2013 ◽

Vol 28 (3) ◽

pp. 320-323 ◽

Cited By ~ 1

Author(s):

Buyun Chen ◽

Guanhua Chen ◽

Michael G. Bartlett

Keyword(s):

Fluorescence Detection ◽

Gel Electrophoresis ◽

Laser Induced Fluorescence ◽

Capillary Gel Electrophoresis ◽

Laser Induced Fluorescence Detection ◽

Factors Influencing ◽

Electrokinetic Injection

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